Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HH195_RS07120 Genome accession   NZ_CP051754
Coordinates   1583166..1584218 (-) Length   350 a.a.
NCBI ID   WP_112365634.1    Uniprot ID   -
Organism   Sarcina sp. JB2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1578166..1589218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH195_RS07100 (HH195_07100) sigE 1578981..1579688 (-) 708 WP_055257224.1 RNA polymerase sporulation sigma factor SigE -
  HH195_RS07105 (HH195_07105) spoIIGA 1579715..1580521 (-) 807 WP_196370450.1 sigma-E processing peptidase SpoIIGA -
  HH195_RS07110 (HH195_07110) ftsZ 1580692..1581831 (-) 1140 WP_058992018.1 cell division protein FtsZ -
  HH195_RS07115 (HH195_07115) ftsA 1581845..1583107 (-) 1263 WP_196370452.1 cell division protein FtsA -
  HH195_RS07120 (HH195_07120) pilT 1583166..1584218 (-) 1053 WP_112365634.1 type IV pilus twitching motility protein PilT Machinery gene
  HH195_RS07125 (HH195_07125) sigK 1584387..1585094 (-) 708 WP_196370454.1 RNA polymerase sporulation sigma factor SigK -
  HH195_RS07130 (HH195_07130) - 1585164..1586777 (-) 1614 WP_196370456.1 penicillin-binding transpeptidase domain-containing protein -
  HH195_RS07135 (HH195_07135) udk 1586833..1587474 (-) 642 WP_196370458.1 uridine kinase -
  HH195_RS07140 (HH195_07140) - 1587477..1588703 (-) 1227 WP_196370460.1 U32 family peptidase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39254.47 Da        Isoelectric Point: 8.2362

>NTDB_id=440863 HH195_RS07120 WP_112365634.1 1583166..1584218(-) (pilT) [Sarcina sp. JB2]
MCSLRELLKIAIKNRASDLHLSVGISPTIRVDGILKKLNYKDLSSDDTEKYAKIILQDQYDIYENQGEFDTVYSINDVGR
FRVNVFKQRGNTSLAIRVIPFEVMSLEELNCPSIVKELCYKKSGLVIVTGPTGSGKSTTLSAMINEINKNSASHIITLED
PIEFLHKHNKSIVNQREIGKDSLSYSKALKSILREDPDVILIGEMRDLETISLAITAAETGHLVFSTLHTIGAVKTIDRI
VDVFPSNQQDQIRIQLANIIEGVISQQLILNKSGKRVAALEIMTSTLAIRNLIREKKTHQIQSLIQSGSKYGMITMDDYL
MKLYRQGTIGKDVLIDFSVDKDMISKSMLY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=440863 HH195_RS07120 WP_112365634.1 1583166..1584218(-) (pilT) [Sarcina sp. JB2]
TTGTGTAGTTTAAGAGAGTTATTAAAAATTGCAATAAAGAATAGAGCTTCCGATTTGCATTTAAGTGTTGGAATATCTCC
AACCATTAGAGTGGATGGAATTTTAAAAAAGCTAAATTACAAAGATTTAAGTTCTGATGATACTGAAAAATATGCTAAAA
TAATATTACAAGACCAATATGATATATATGAAAATCAAGGGGAATTTGATACAGTTTATTCGATAAATGATGTTGGAAGA
TTTAGAGTTAATGTATTTAAACAAAGAGGTAATACATCTTTAGCTATAAGAGTTATACCTTTTGAAGTAATGAGTTTAGA
AGAATTAAACTGCCCTAGTATTGTAAAAGAATTATGTTATAAAAAAAGTGGACTAGTTATTGTAACTGGACCAACAGGTA
GTGGTAAAAGTACAACTTTATCGGCTATGATAAATGAGATAAATAAAAATAGTGCAAGTCATATTATAACTTTAGAGGAT
CCAATAGAGTTTTTACATAAACATAATAAATCTATTGTAAACCAAAGAGAAATAGGTAAGGATTCTCTAAGTTACAGTAA
AGCCTTAAAATCTATCTTAAGAGAGGATCCAGATGTTATATTGATTGGAGAGATGAGAGATTTAGAAACCATATCACTAG
CAATAACAGCAGCAGAAACAGGACATCTAGTTTTTTCTACTCTTCATACTATAGGTGCAGTTAAAACTATAGATAGAATT
GTAGATGTATTTCCTAGTAATCAACAGGATCAAATAAGAATTCAGTTAGCTAATATAATAGAGGGAGTAATTTCACAACA
GTTAATTTTAAATAAAAGCGGAAAAAGAGTTGCTGCATTAGAAATAATGACTTCTACTTTAGCTATTAGAAATCTAATAA
GAGAGAAAAAGACTCATCAAATACAATCATTAATTCAGTCTGGAAGTAAATATGGGATGATAACTATGGATGATTATTTA
ATGAAATTATATAGGCAAGGGACAATAGGAAAAGATGTGTTAATAGATTTTTCAGTTGATAAAGATATGATAAGTAAAAG
CATGCTATATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

50.588

97.143

0.491
  pilT Legionella pneumophila strain ERS1305867

50.588

97.143

0.491
  pilT Neisseria meningitidis 8013

51.818

94.286

0.489
  pilT Neisseria gonorrhoeae MS11

51.515

94.286

0.486
  pilT Acinetobacter baylyi ADP1

51.692

92.857

0.48
  pilT Pseudomonas aeruginosa PAK

50.61

93.714

0.474
  pilT Acinetobacter nosocomialis M2

51.077

92.857

0.474
  pilT Acinetobacter baumannii D1279779

51.077

92.857

0.474
  pilT Acinetobacter baumannii strain A118

51.077

92.857

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.462

92.857

0.469
  pilT Vibrio cholerae strain A1552

50.462

92.857

0.469
  pilT Pseudomonas stutzeri DSM 10701

49.695

93.714

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.23

98

0.463
  pilU Pseudomonas stutzeri DSM 10701

38.372

98.286

0.377
  pilU Vibrio cholerae strain A1552

39.877

93.143

0.371
  pilU Acinetobacter baylyi ADP1

38.906

94

0.366