Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KPHS_44540 Genome accession   NC_016845
Coordinates   4489111..4490091 (-) Length   326 a.a.
NCBI ID   YP_005228754.1    Uniprot ID   -
Organism   Klebsiella pneumoniae subsp. pneumoniae HS11286     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4484111..4495091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KPHS_44470 - 4484401..4485108 (+) 708 YP_005228747.1 DNA-specific endonuclease I -
  KPHS_44480 - 4485200..4485931 (+) 732 YP_005228748.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KPHS_44490 - 4485952..4486902 (+) 951 YP_005228749.1 glutathione synthetase -
  KPHS_44500 - 4487077..4487640 (+) 564 YP_005228750.1 YqgE/AlgH family protein -
  KPHS_44510 - 4487640..4488056 (+) 417 YP_005228751.1 Holliday junction resolvase-like protein -
  KPHS_44520 - 4488109..4488762 (-) 654 YP_005228752.1 DNA-binding transcriptional regulator CsgD -
  KPHS_44530 - 4488917..4489060 (+) 144 YP_005228753.1 hypothetical protein -
  KPHS_44540 pilT 4489111..4490091 (-) 981 YP_005228754.1 type IV pilus twitching motility protein PilT Machinery gene
  KPHS_44550 - 4490108..4490809 (+) 702 YP_005228755.1 PLP-binding domain-containing protein -
  KPHS_44560 - 4490878..4491396 (+) 519 YP_005228756.1 YggT family protein -
  KPHS_44570 - 4491393..4491683 (+) 291 YP_005228757.1 YggU family protein -
  KPHS_44580 - 4491696..4492289 (+) 594 YP_005228758.1 putative deoxyribonucleotide triphosphate pyrophosphatase -
  KPHS_44590 - 4492282..4493418 (+) 1137 YP_005228759.1 coproporphyrinogen III oxidase -
  KPHS_44600 - 4493459..4494178 (-) 720 YP_005228760.1 DUF2884 domain-containing protein -
  KPHS_44610 - 4494229..4494555 (-) 327 YP_005228761.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35260.74 Da        Isoelectric Point: 6.7288

>NTDB_id=43804 KPHS_44540 YP_005228754.1 4489111..4490091(-) (pilT) [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MKLEEIVALSVKHNVSDLHLCNSAAPRWWRQGRLQPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALTLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDTLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQC
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=43804 KPHS_44540 YP_005228754.1 4489111..4490091(-) (pilT) [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCACG
CTGGTGGCGGCAGGGCAGGCTGCAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGCCTCCTTCCTGAACAGTGCCCGCGTCTGGACACGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGATAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTAATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTACTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAATGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.064

100

0.515
  pilT Vibrio cholerae strain A1552

51.064

100

0.515
  pilT Acinetobacter baylyi ADP1

48.632

100

0.491
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

47.72

100

0.482
  pilT Acinetobacter baumannii strain A118

47.72

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

47.416

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.84

99.387

0.475
  pilT Legionella pneumophila strain Lp02

47.84

99.387

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.231

99.693

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.875

98.466

0.393

Multiple sequence alignment