Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HFT84_RS18895 Genome accession   NZ_CP051320
Coordinates   3898082..3899062 (-) Length   326 a.a.
NCBI ID   WP_001055640.1    Uniprot ID   A0A5Z3PPJ3
Organism   Salmonella enterica subsp. enterica serovar Muenster strain CVM 34526     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3893082..3904062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HFT84_RS18865 (HFT84_18860) endA 3893540..3894247 (+) 708 WP_000286123.1 deoxyribonuclease I -
  HFT84_RS18870 (HFT84_18865) rsmE 3894324..3895055 (+) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HFT84_RS18875 (HFT84_18870) gshB 3895075..3896022 (+) 948 WP_023137233.1 glutathione synthase -
  HFT84_RS18880 (HFT84_18875) - 3896238..3896801 (+) 564 WP_001053167.1 YqgE/AlgH family protein -
  HFT84_RS18885 (HFT84_18880) ruvX 3896801..3897217 (+) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  HFT84_RS18890 (HFT84_18885) - 3897264..3897950 (-) 687 WP_000098339.1 IclR family transcriptional regulator domain-containing protein -
  HFT84_RS18895 (HFT84_18890) pilT 3898082..3899062 (-) 981 WP_001055640.1 type IV pilus twitching motility protein PilT Machinery gene
  HFT84_RS18900 (HFT84_18895) - 3899080..3899784 (+) 705 WP_023137234.1 YggS family pyridoxal phosphate-dependent enzyme -
  HFT84_RS18905 (HFT84_18900) - 3899803..3900369 (+) 567 WP_001094848.1 YggT family protein -
  HFT84_RS18910 (HFT84_18905) yggU 3900366..3900656 (+) 291 WP_001277203.1 DUF167 family protein YggU -
  HFT84_RS18915 (HFT84_18910) - 3900664..3901257 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  HFT84_RS18920 (HFT84_18915) hemW 3901250..3902386 (+) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  HFT84_RS18925 (HFT84_18920) - 3902477..3903484 (-) 1008 WP_023137235.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36205.45 Da        Isoelectric Point: 7.5116

>NTDB_id=437504 HFT84_RS18895 WP_001055640.1 3898082..3899062(-) (pilT) [Salmonella enterica subsp. enterica serovar Muenster strain CVM 34526]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFIHQQGTSLALRLLPERCPDLAEIQTPPIVPVLLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPIRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=437504 HFT84_RS18895 WP_001055640.1 3898082..3899062(-) (pilT) [Salmonella enterica subsp. enterica serovar Muenster strain CVM 34526]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCATACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGTACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAA
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACTGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGATACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Z3PPJ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365