Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HFU03_RS03830 Genome accession   NZ_CP051315
Coordinates   785230..786210 (+) Length   326 a.a.
NCBI ID   WP_001653704.1    Uniprot ID   A0A3V4SJJ4
Organism   Salmonella enterica subsp. enterica serovar Uganda strain CVM 35009     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 780230..791210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HFU03_RS03800 (HFU03_03800) - 780807..781814 (+) 1008 WP_000252200.1 DUF1202 domain-containing protein -
  HFU03_RS03805 (HFU03_03805) hemW 781906..783042 (-) 1137 WP_079798516.1 radical SAM family heme chaperone HemW -
  HFU03_RS03810 (HFU03_03810) - 783035..783628 (-) 594 WP_001174764.1 XTP/dITP diphosphatase -
  HFU03_RS03815 (HFU03_03815) yggU 783636..783926 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  HFU03_RS03820 (HFU03_03820) - 783923..784489 (-) 567 WP_001094848.1 YggT family protein -
  HFU03_RS03825 (HFU03_03825) - 784508..785212 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  HFU03_RS03830 (HFU03_03830) pilT 785230..786210 (+) 981 WP_001653704.1 type IV pilus twitching motility protein PilT Machinery gene
  HFU03_RS03835 (HFU03_03835) - 786345..787031 (+) 687 WP_000098329.1 IclR family transcriptional regulator -
  HFU03_RS03840 (HFU03_03840) ruvX 787078..787494 (-) 417 WP_001014572.1 Holliday junction resolvase RuvX -
  HFU03_RS03845 (HFU03_03845) - 787494..788057 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  HFU03_RS03850 (HFU03_03850) gshB 788273..789220 (-) 948 WP_000593248.1 glutathione synthase -
  HFU03_RS03855 (HFU03_03855) rsmE 789240..789971 (-) 732 WP_023203985.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HFU03_RS03860 (HFU03_03860) endA 790048..790755 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36179.37 Da        Isoelectric Point: 7.5116

>NTDB_id=437442 HFU03_RS03830 WP_001653704.1 785230..786210(+) (pilT) [Salmonella enterica subsp. enterica serovar Uganda strain CVM 35009]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=437442 HFU03_RS03830 WP_001653704.1 785230..786210(+) (pilT) [Salmonella enterica subsp. enterica serovar Uganda strain CVM 35009]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGAGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3V4SJJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

46.395

97.853

0.454
  pilT Pseudomonas stutzeri DSM 10701

46.203

96.933

0.448
  pilT Pseudomonas aeruginosa PAK

46.203

96.933

0.448
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.858

100

0.374
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362