Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HFU35_RS03965 Genome accession   NZ_CP051307
Coordinates   811138..812118 (+) Length   326 a.a.
NCBI ID   WP_001055653.1    Uniprot ID   A0A7U1KVG4
Organism   Salmonella enterica subsp. enterica serovar Reading strain CVM 35189     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 806138..817118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HFU35_RS03935 (HFU35_03940) - 806716..807723 (+) 1008 WP_000252197.1 DUF1202 domain-containing protein -
  HFU35_RS03940 (HFU35_03945) hemW 807814..808950 (-) 1137 WP_001096521.1 radical SAM family heme chaperone HemW -
  HFU35_RS03945 (HFU35_03950) - 808943..809536 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  HFU35_RS03950 (HFU35_03955) yggU 809544..809834 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  HFU35_RS03955 (HFU35_03960) - 809831..810397 (-) 567 WP_001094848.1 YggT family protein -
  HFU35_RS03960 (HFU35_03965) - 810416..811120 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  HFU35_RS03965 (HFU35_03970) pilT 811138..812118 (+) 981 WP_001055653.1 type IV pilus twitching motility protein PilT Machinery gene
  HFU35_RS03970 (HFU35_03975) - 812250..812936 (+) 687 WP_023243685.1 IclR family transcriptional regulator -
  HFU35_RS03975 (HFU35_03980) ruvX 812982..813398 (-) 417 WP_001014571.1 Holliday junction resolvase RuvX -
  HFU35_RS03980 (HFU35_03985) - 813398..813961 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  HFU35_RS03985 (HFU35_03990) gshB 814177..815124 (-) 948 WP_058652998.1 glutathione synthase -
  HFU35_RS03990 (HFU35_03995) rsmE 815144..815875 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HFU35_RS03995 (HFU35_04000) endA 815952..816659 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36198.38 Da        Isoelectric Point: 7.2931

>NTDB_id=437375 HFU35_RS03965 WP_001055653.1 811138..812118(+) (pilT) [Salmonella enterica subsp. enterica serovar Reading strain CVM 35189]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSDTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=437375 HFU35_RS03965 WP_001055653.1 811138..812118(+) (pilT) [Salmonella enterica subsp. enterica serovar Reading strain CVM 35189]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACTGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGACACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAACGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U1KVG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

45.455

97.853

0.445
  pilT Pseudomonas aeruginosa PAK

45.57

96.933

0.442
  pilT Pseudomonas stutzeri DSM 10701

45.57

96.933

0.442
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362