Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   INR48_RS17890 Genome accession   NZ_CP063153
Coordinates   3760243..3761223 (-) Length   326 a.a.
NCBI ID   WP_001389259.1    Uniprot ID   -
Organism   Escherichia coli strain M7424     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3755243..3766223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR48_RS17860 (INR48_17865) yggI 3755907..3756404 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  INR48_RS17865 (INR48_17870) endA 3756499..3757206 (+) 708 WP_000286517.1 deoxyribonuclease I -
  INR48_RS17870 (INR48_17875) rsmE 3757286..3758017 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  INR48_RS17875 (INR48_17880) gshB 3758030..3758980 (+) 951 WP_000593273.1 glutathione synthase -
  INR48_RS17880 (INR48_17885) yqgE 3759089..3759652 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  INR48_RS17885 (INR48_17890) ruvX 3759652..3760068 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  INR48_RS17890 (INR48_17895) pilT 3760243..3761223 (-) 981 WP_001389259.1 type IV pilus twitching motility protein PilT Machinery gene
  INR48_RS17895 (INR48_17900) yggS 3761241..3761945 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  INR48_RS17900 (INR48_17905) yggT 3761963..3762529 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  INR48_RS17905 (INR48_17910) yggU 3762526..3762816 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  INR48_RS17910 (INR48_17915) rdgB 3762824..3763417 (+) 594 WP_001174735.1 XTP/dITP diphosphatase -
  INR48_RS17915 (INR48_17920) hemW 3763410..3764546 (+) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  INR48_RS17920 (INR48_17925) yggM 3764701..3765708 (-) 1008 WP_000745217.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35984.20 Da        Isoelectric Point: 5.7980

>NTDB_id=437374 INR48_RS17890 WP_001389259.1 3760243..3761223(-) (pilT) [Escherichia coli strain M7424]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAVQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=437374 INR48_RS17890 WP_001389259.1 3760243..3761223(-) (pilT) [Escherichia coli strain M7424]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCATTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGCTGTTGCCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGAAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGCGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACACCTGGTACTGGCAACCTTACATACACGTGGTGCCGTGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCAGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365