Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HEP74_RS05265 Genome accession   NZ_CP051190
Coordinates   1250093..1250500 (+) Length   135 a.a.
NCBI ID   WP_185815508.1    Uniprot ID   -
Organism   Xanthomonas sp. SS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1245093..1255500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP74_RS05245 (HEP74_01034) - 1245350..1246696 (+) 1347 WP_185815505.1 HAMP domain-containing sensor histidine kinase -
  HEP74_RS05250 (HEP74_01035) coaE 1246944..1247564 (-) 621 WP_185815506.1 dephospho-CoA kinase -
  HEP74_RS05255 (HEP74_01036) - 1247578..1248441 (-) 864 WP_046978035.1 A24 family peptidase -
  HEP74_RS05260 (HEP74_01037) pilC 1248448..1249707 (-) 1260 WP_185815507.1 type II secretion system F family protein Machinery gene
  HEP74_RS05265 (HEP74_01038) pilA 1250093..1250500 (+) 408 WP_185815508.1 pilin Machinery gene
  HEP74_RS22015 - 1250589..1250999 (+) 411 WP_185815509.1 pilin -
  HEP74_RS05275 (HEP74_01039) pilB 1251037..1252776 (+) 1740 WP_185815510.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HEP74_RS05280 (HEP74_01040) pilR 1252931..1254319 (-) 1389 WP_185815511.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14175.43 Da        Isoelectric Point: 8.1105

>NTDB_id=436749 HEP74_RS05265 WP_185815508.1 1250093..1250500(+) (pilA) [Xanthomonas sp. SS]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQVAAGLAEITPGKTQFEVMVNDGKVATITPDLIGLKTSDRCTV
TVNATETGSITCTLVNSSPKVNNATIVWTRAADGTWACSTTVSDKAKWAPATCQG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=436749 HEP74_RS05265 WP_185815508.1 1250093..1250500(+) (pilA) [Xanthomonas sp. SS]
ATGAAGAAGCAGCAGGGCTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGGCTTACCAGAACTACGTCGCCAAGTCGCAGGTCGCCGCTGGTCTCGCTGAAATCACCCCCGGCAAAACGCAGTTCG
AAGTGATGGTCAACGACGGTAAGGTCGCCACCATCACCCCCGATCTGATCGGCCTGAAGACCTCCGATCGCTGCACTGTT
ACCGTGAATGCTACGGAAACCGGCAGCATCACGTGCACCTTGGTGAATTCCAGCCCGAAGGTGAACAATGCAACCATAGT
CTGGACCCGTGCTGCTGACGGTACCTGGGCTTGCAGCACGACGGTCAGTGACAAGGCCAAGTGGGCGCCGGCTACTTGCC
AGGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

51.064

100

0.533
  pilA/pilAI Pseudomonas stutzeri DSM 10701

49.63

100

0.496
  pilA/pilAII Pseudomonas stutzeri DSM 10701

50

99.259

0.496
  pilA Pseudomonas aeruginosa PAK

41.447

100

0.467
  comP Acinetobacter baylyi ADP1

40.268

100

0.444
  pilA Ralstonia pseudosolanacearum GMI1000

34.731

100

0.43
  pilA Vibrio cholerae C6706

39.437

100

0.415
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.437

100

0.415
  pilA Vibrio cholerae strain A1552

39.437

100

0.415
  pilA Vibrio parahaemolyticus RIMD 2210633

41.221

97.037

0.4
  pilA2 Legionella pneumophila str. Paris

38.571

100

0.4
  pilA/pilA1 Eikenella corrodens VA1

32.317

100

0.393
  pilE Neisseria gonorrhoeae MS11

37.226

100

0.378