Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HEP74_RS05275 Genome accession   NZ_CP051190
Coordinates   1251037..1252776 (+) Length   579 a.a.
NCBI ID   WP_185815510.1    Uniprot ID   -
Organism   Xanthomonas sp. SS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1246037..1257776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP74_RS05250 (HEP74_01035) coaE 1246944..1247564 (-) 621 WP_185815506.1 dephospho-CoA kinase -
  HEP74_RS05255 (HEP74_01036) - 1247578..1248441 (-) 864 WP_046978035.1 A24 family peptidase -
  HEP74_RS05260 (HEP74_01037) pilC 1248448..1249707 (-) 1260 WP_185815507.1 type II secretion system F family protein Machinery gene
  HEP74_RS05265 (HEP74_01038) pilA 1250093..1250500 (+) 408 WP_185815508.1 pilin Machinery gene
  HEP74_RS22015 - 1250589..1250999 (+) 411 WP_185815509.1 pilin -
  HEP74_RS05275 (HEP74_01039) pilB 1251037..1252776 (+) 1740 WP_185815510.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HEP74_RS05280 (HEP74_01040) pilR 1252931..1254319 (-) 1389 WP_185815511.1 sigma-54 dependent transcriptional regulator Regulator
  HEP74_RS05285 (HEP74_01041) - 1254393..1256006 (-) 1614 WP_185815512.1 HAMP domain-containing sensor histidine kinase -
  HEP74_RS05290 (HEP74_01042) sucC 1256306..1257469 (+) 1164 WP_179566646.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62998.30 Da        Isoelectric Point: 5.7976

>NTDB_id=436750 HEP74_RS05275 WP_185815510.1 1251037..1252776(+) (pilB) [Xanthomonas sp. SS]
MNVATSANLVGITGIARRLVQDGALEEAVARTAMAHAAEAKIPLPQWFSEKKLVTAAQLAAANAVEFGMPLLDVSVFDAN
QSAIKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQARNDTIGNSLG
GDDEGMDNLEVGAGDEDMGGGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEEEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRLAPAM
PENALLKEGFTEAEIAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIADAAQKIGVKDLRQSAL
IKARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=436750 HEP74_RS05275 WP_185815510.1 1251037..1252776(+) (pilB) [Xanthomonas sp. SS]
ATGAATGTAGCGACTTCTGCCAATCTCGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGACGGCGCGCTGGAGGA
AGCGGTCGCGCGCACCGCCATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACCGCGGCGCAGCTGGCTGCCGCCAACGCGGTGGAGTTCGGCATGCCGTTGCTGGATGTCTCGGTGTTCGACGCCAAC
CAGAGCGCGATCAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTCGGGGTCAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCGCGCAACGATACGATCGGCAATTCGCTGGGC
GGCGACGACGAGGGCATGGACAACCTCGAGGTCGGGGCCGGCGACGAGGACATGGGCGGCGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCGGTGGTCAAGTTCGTGAACAAGATGCTGGTCGACGCGATCCGTCGCGGCGCCTCGGACA
TCCACTTCGAGCCGTACGAGGACGACTATCGGGTGCGCTTGCGCATCGATGGCCTGCTCAAGAGCGTGGCCAAGGCGCCG
GTCAAGCTCAACCAGCGCATCGCGGCGCGGTTGAAGGTGATGTCGCAGTTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACGCTGTTCGGTGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTATGAGGAAGAGCAACAGAAG
CTGTTCCTGGACGCGATCCACAAGCCGTATGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATTTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGTTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACTGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGCTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGCTATGCAACAACTGCAAGCGGCTGGCCCCGGCCATG
CCGGAGAACGCGCTGTTGAAGGAAGGCTTCACCGAGGCCGAAATCGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTG
CGACGAATGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCG
TACTGCAGGGCGGCAACGCGATGGACATCGCCGATGCGGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCGCTG
ATCAAGGCGCGCGCCGGCATCACCAGCTTGGCCGAGATCAATCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-151)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.148

98.964

0.546
  pilB Acinetobacter baylyi ADP1

54.561

98.446

0.537
  pilB Legionella pneumophila strain ERS1305867

52.373

98.273

0.515
  pilF Neisseria gonorrhoeae MS11

48.592

98.1

0.477
  pilB Vibrio cholerae strain A1552

51.206

93.092

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

46.881

96.891

0.454
  pilB Vibrio campbellii strain DS40M4

46.14

98.446

0.454
  pilF Thermus thermophilus HB27

40.114

90.846

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.307

89.983

0.363