Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HF520_RS14140 Genome accession   NZ_CP051144
Coordinates   2904960..2906021 (+) Length   353 a.a.
NCBI ID   WP_168574524.1    Uniprot ID   A0A6H1X3F8
Organism   Romboutsia sp. CE17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2899960..2911021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF520_RS14120 (HF520_14120) purR 2900285..2901175 (+) 891 WP_168574520.1 pur operon repressor -
  HF520_RS14125 (HF520_14125) spoVG 2901300..2901578 (+) 279 WP_168574521.1 septation regulator SpoVG -
  HF520_RS14130 (HF520_14130) glmU 2901831..2903204 (+) 1374 WP_168574522.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  HF520_RS14135 (HF520_14135) - 2903297..2904247 (+) 951 WP_168574523.1 ribose-phosphate diphosphokinase -
  HF520_RS14140 (HF520_14140) pilT 2904960..2906021 (+) 1062 WP_168574524.1 type IV pilus twitching motility protein PilT Machinery gene
  HF520_RS14145 (HF520_14145) - 2906316..2906954 (+) 639 WP_168574525.1 A24 family peptidase -
  HF520_RS14150 (HF520_14150) pth 2906968..2907528 (+) 561 WP_168574526.1 aminoacyl-tRNA hydrolase -
  HF520_RS14155 (HF520_14155) mfd 2907547..2910939 (+) 3393 WP_168574527.1 transcription-repair coupling factor -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39634.41 Da        Isoelectric Point: 6.2595

>NTDB_id=436205 HF520_RS14140 WP_168574524.1 2904960..2906021(+) (pilT) [Romboutsia sp. CE17]
MNIFDLLEIAIEIGASDIHITVGSAPIARINGKFIKLNDRILTSEDTEDMTKDIAKENKFKRIEMYGECDFSVALETGER
FRVNAYKQKGNYALAIRTIMSTIPSFESLGLPEVLKTFTERNKGLVLVTGPTGSGKSTTLASLIDIINENQQKHIITIED
PIEYVHKHKMGLVNQREIGQDTKSFNSALRSILRQDPDVILVGEMRDAETISIALTAAETGHLVFSTLHTVGAAKTIDRI
VDMFPSEQQKQIRSQLSTVCEGIVSQQLIQTIDGRRRVAALEVMIANSAIRNLIRENKTYQIQNQIQTGSKFGMQSMDQE
LVNLYIQGIISRNSVLSRCTDYEYTIRLIEDKY

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=436205 HF520_RS14140 WP_168574524.1 2904960..2906021(+) (pilT) [Romboutsia sp. CE17]
ATGAATATATTTGATTTATTAGAAATTGCAATAGAGATAGGAGCTTCAGATATACATATAACAGTAGGCAGTGCGCCTAT
TGCTAGAATAAATGGTAAGTTTATAAAATTAAATGATAGGATATTAACTAGTGAAGATACTGAAGATATGACAAAGGATA
TAGCGAAGGAAAATAAATTTAAAAGGATAGAGATGTATGGTGAATGTGATTTCTCAGTAGCTTTAGAGACTGGAGAACGA
TTTAGAGTAAATGCATATAAGCAAAAAGGAAATTATGCTCTAGCAATAAGAACGATAATGTCTACTATACCATCATTTGA
ATCTTTAGGGCTTCCAGAAGTACTAAAGACATTTACAGAAAGAAATAAAGGGCTAGTCTTAGTTACTGGACCGACAGGTA
GTGGAAAATCAACAACACTTGCAAGTCTTATAGATATAATTAATGAAAATCAGCAAAAACATATAATAACTATTGAAGAT
CCAATAGAATACGTACATAAGCATAAAATGGGATTAGTAAATCAAAGAGAAATAGGACAAGATACAAAAAGTTTTAATTC
TGCACTAAGATCCATACTTCGTCAAGATCCAGATGTAATATTGGTAGGGGAGATGAGAGATGCAGAAACTATATCAATTG
CATTAACTGCAGCAGAAACAGGACATTTGGTATTTTCTACATTACATACAGTTGGTGCAGCAAAGACGATAGATAGAATA
GTAGATATGTTTCCATCTGAACAGCAGAAACAAATAAGAAGTCAATTATCAACTGTTTGTGAGGGAATAGTTTCACAACA
ACTAATACAAACTATAGATGGAAGGCGAAGAGTTGCAGCGCTTGAGGTTATGATTGCAAATTCAGCAATTAGAAATCTAA
TAAGAGAAAATAAAACATATCAAATACAAAATCAAATACAGACAGGTAGCAAATTTGGAATGCAGTCTATGGATCAAGAA
TTAGTCAATTTATATATACAAGGAATAATTTCTAGAAATAGTGTTTTAAGTAGATGTACGGACTATGAATATACAATTAG
ATTGATAGAAGATAAATATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H1X3F8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

51.02

97.167

0.496
  pilT Neisseria gonorrhoeae MS11

50.729

97.167

0.493
  pilT Legionella pneumophila strain Lp02

51.198

94.618

0.484
  pilT Legionella pneumophila strain ERS1305867

51.198

94.618

0.484
  pilT Pseudomonas aeruginosa PAK

50.898

94.618

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.271

97.167

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.148

99.433

0.479
  pilT Acinetobacter baumannii strain A118

50.151

93.768

0.47
  pilT Acinetobacter baumannii D1279779

50.151

93.768

0.47
  pilT Acinetobacter nosocomialis M2

50.151

93.768

0.47
  pilT Vibrio cholerae strain A1552

49.112

95.751

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.112

95.751

0.47
  pilT Acinetobacter baylyi ADP1

48.338

93.768

0.453
  pilU Acinetobacter baylyi ADP1

39.828

98.867

0.394
  pilU Pseudomonas stutzeri DSM 10701

41.071

95.184

0.391
  pilU Vibrio cholerae strain A1552

39.349

95.751

0.377