Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HB664_RS22065 Genome accession   NZ_CP050520
Coordinates   4587975..4588955 (-) Length   326 a.a.
NCBI ID   WP_219940216.1    Uniprot ID   -
Organism   Enterobacter sp. DNB-S2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4582975..4593955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB664_RS22035 (HB664_22055) - 4583851..4584348 (+) 498 WP_033146604.1 SprT family zinc-dependent metalloprotease -
  HB664_RS22040 (HB664_22060) endA 4584443..4585150 (+) 708 WP_023333343.1 deoxyribonuclease I -
  HB664_RS22045 (HB664_22065) rsmE 4585202..4585933 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HB664_RS22050 (HB664_22070) gshB 4585953..4586900 (+) 948 WP_219940215.1 glutathione synthase -
  HB664_RS22055 (HB664_22075) - 4586988..4587548 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  HB664_RS22060 (HB664_22080) ruvX 4587548..4587964 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  HB664_RS22065 (HB664_22085) pilT 4587975..4588955 (-) 981 WP_219940216.1 type IV pilus twitching motility protein PilT Machinery gene
  HB664_RS22070 (HB664_22090) - 4588973..4589674 (+) 702 WP_058841911.1 YggS family pyridoxal phosphate-dependent enzyme -
  HB664_RS22075 (HB664_22095) - 4589696..4590262 (+) 567 WP_033146608.1 YggT family protein -
  HB664_RS22080 (HB664_22100) yggU 4590259..4590555 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  HB664_RS22085 (HB664_22105) - 4590559..4591152 (+) 594 WP_219940217.1 XTP/dITP diphosphatase -
  HB664_RS22090 (HB664_22110) hemW 4591145..4592281 (+) 1137 WP_219940218.1 radical SAM family heme chaperone HemW -
  HB664_RS22095 (HB664_22115) - 4592351..4592689 (+) 339 WP_219940219.1 endonuclease domain-containing protein -
  HB664_RS22100 (HB664_22120) - 4592772..4593488 (-) 717 WP_058841913.1 DUF2884 domain-containing protein -
  HB664_RS22105 (HB664_22125) - 4593545..4593871 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35504.59 Da        Isoelectric Point: 6.9108

>NTDB_id=433601 HB664_RS22065 WP_219940216.1 4587975..4588955(-) (pilT) [Enterobacter sp. DNB-S2]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFQPPDVGALLKAWLNDEQQGIWWAKGQVDFAATVTGGPRLRG
SAFKQMRGVSVTLRLLPRSCPQLASLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=433601 HB664_RS22065 WP_219940216.1 4587975..4588955(-) (pilT) [Enterobacter sp. DNB-S2]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGGATATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCCGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTTTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Pseudomonas aeruginosa PAK

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

98.773

0.436
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362