Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HCG50_RS11030 Genome accession   NZ_CP050447
Coordinates   2339757..2340779 (-) Length   340 a.a.
NCBI ID   WP_167324600.1    Uniprot ID   -
Organism   Serratia marcescens strain SER00094     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2334757..2345779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCG50_RS11000 (HCG50_11000) - 2335556..2336068 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  HCG50_RS11005 (HCG50_11005) endA 2336170..2336865 (+) 696 WP_089196263.1 deoxyribonuclease I -
  HCG50_RS11010 (HCG50_11010) rsmE 2336935..2337666 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HCG50_RS11015 (HCG50_11015) gshB 2337677..2338627 (+) 951 WP_025304245.1 glutathione synthase -
  HCG50_RS11020 (HCG50_11020) - 2338775..2339338 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  HCG50_RS11025 (HCG50_11025) ruvX 2339338..2339760 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  HCG50_RS11030 (HCG50_11030) pilT 2339757..2340779 (-) 1023 WP_167324600.1 type IV pilus twitching motility protein PilT Machinery gene
  HCG50_RS11035 (HCG50_11035) - 2340800..2341507 (+) 708 WP_025304248.1 YggS family pyridoxal phosphate-dependent enzyme -
  HCG50_RS11040 (HCG50_11040) proC 2341527..2342348 (+) 822 WP_025304249.1 pyrroline-5-carboxylate reductase -
  HCG50_RS11045 (HCG50_11045) - 2342380..2342934 (+) 555 WP_025304250.1 YggT family protein -
  HCG50_RS11050 (HCG50_11050) yggU 2342931..2343221 (+) 291 WP_167324601.1 DUF167 family protein YggU -
  HCG50_RS11055 (HCG50_11055) - 2343239..2343832 (+) 594 WP_025304251.1 XTP/dITP diphosphatase -
  HCG50_RS11060 (HCG50_11060) hemW 2343825..2344967 (+) 1143 WP_167324602.1 radical SAM family heme chaperone HemW -
  HCG50_RS11065 (HCG50_11065) - 2345007..2345441 (-) 435 WP_167324603.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36501.74 Da        Isoelectric Point: 7.0670

>NTDB_id=432725 HCG50_RS11030 WP_167324600.1 2339757..2340779(-) (pilT) [Serratia marcescens strain SER00094]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAVAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGMSLALRRIAGQSPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=432725 HCG50_RS11030 WP_167324600.1 2339757..2340779(-) (pilT) [Serratia marcescens strain SER00094]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGTATCGACGGTGAACTGCAGCCGCTGGCGGTGGCGCCAACGCTGACGGCACAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGTGGAGAG
CGGCTGCGTGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGTCGCCCTCGCT
TGCCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTCGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

55.152

97.059

0.535
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.152

97.059

0.535
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Neisseria meningitidis 8013

50.301

97.647

0.491
  pilT Neisseria gonorrhoeae MS11

49.702

98.824

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.665

99.118

0.482
  pilT Legionella pneumophila strain ERS1305867

49.547

97.353

0.482
  pilT Legionella pneumophila strain Lp02

49.547

97.353

0.482
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Vibrio cholerae strain A1552

40.432

95.294

0.385
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368