Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DESAF_RS04425 Genome accession   NC_016629
Coordinates   962829..963914 (-) Length   361 a.a.
NCBI ID   WP_014259053.1    Uniprot ID   F3YUY3
Organism   Desulfocurvibacter africanus subsp. africanus str. Walvis Bay     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 957829..968914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESAF_RS04400 (Desaf_0879) - 958404..958799 (+) 396 WP_014259047.1 phage holin family protein -
  DESAF_RS04405 (Desaf_0880) - 958775..959917 (+) 1143 WP_014259048.1 DUF3618 domain-containing protein -
  DESAF_RS04410 (Desaf_0881) sugE 960190..960516 (+) 327 WP_014259049.1 quaternary ammonium compound efflux SMR transporter SugE -
  DESAF_RS04415 (Desaf_0882) - 960558..961358 (-) 801 WP_014259050.1 hypothetical protein -
  DESAF_RS19715 (Desaf_0883) - 961745..962116 (+) 372 WP_014259051.1 cupin domain-containing protein -
  DESAF_RS04420 (Desaf_0884) - 961999..962586 (-) 588 WP_014259052.1 GNAT family N-acetyltransferase -
  DESAF_RS04425 (Desaf_0885) pilT 962829..963914 (-) 1086 WP_014259053.1 type IV pilus twitching motility protein PilT Machinery gene
  DESAF_RS04430 (Desaf_0886) - 963959..965644 (-) 1686 WP_014259054.1 polymer-forming cytoskeletal protein -
  DESAF_RS04435 (Desaf_0887) - 965655..966164 (-) 510 WP_014259055.1 PulJ/GspJ family protein -
  DESAF_RS04440 (Desaf_0888) - 966167..966670 (-) 504 WP_014259056.1 PulJ/GspJ family protein -
  DESAF_RS04445 (Desaf_0889) - 966667..967143 (-) 477 WP_014259057.1 type II secretion system protein -
  DESAF_RS04450 (Desaf_0890) - 967217..967615 (-) 399 WP_014259058.1 type II secretion system protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40113.31 Da        Isoelectric Point: 6.7777

>NTDB_id=43190 DESAF_RS04425 WP_014259053.1 962829..963914(-) (pilT) [Desulfocurvibacter africanus subsp. africanus str. Walvis Bay]
MPRIETFLRKLMEEGGSDLHLSSGAQPIIRLHGKLEPLGREPMVPAELDELLREIAREDKIREFEACGDVDFGYEIKGLG
RFRVNYFRQMRGMSAAFRVVPERVPSMDELRLPAILKDLAMLPKGLVLVTGPTGSGKSTTLAAMIDHANQHRREHIITIE
DPVEFVHQSKGCLVQQREVGRDTKSFQAALRGALREDPDIILVGEMRDRETIALALEAAETGHLVLSTLHTVSAAKTVDR
IIEVFPGDTQGQIRSSLSESLRAVVSQVLFTRADRPGRVAALELLLAVPAVRNLIRENKTYQLPSLIQMGKAQGMQHLDE
QIQKLLDSGMITPEDALAQAVDKKRFARQGDGRLMGSMFSE

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=43190 DESAF_RS04425 WP_014259053.1 962829..963914(-) (pilT) [Desulfocurvibacter africanus subsp. africanus str. Walvis Bay]
GTGCCGCGTATCGAAACCTTCCTGCGCAAGCTCATGGAAGAGGGCGGTTCGGACCTGCACCTGTCCAGCGGCGCGCAGCC
CATCATTCGCCTGCACGGCAAGCTGGAGCCCCTAGGCCGCGAGCCCATGGTTCCAGCGGAGTTGGACGAACTGCTGCGCG
AGATCGCACGCGAGGACAAGATCCGGGAATTCGAGGCCTGCGGGGATGTGGATTTCGGCTACGAAATCAAGGGGCTGGGC
CGCTTCCGCGTGAACTACTTCCGCCAGATGCGCGGCATGTCCGCGGCGTTTCGCGTGGTGCCCGAGCGCGTGCCGAGCAT
GGACGAACTGCGCCTGCCGGCAATCCTCAAGGACTTGGCCATGCTGCCCAAGGGGCTTGTGCTCGTGACCGGGCCAACGG
GCAGCGGCAAGTCCACCACCCTGGCGGCCATGATCGACCACGCCAACCAGCACCGCCGCGAGCACATCATCACCATCGAG
GACCCGGTGGAGTTCGTGCACCAGAGCAAGGGCTGCCTGGTGCAGCAGCGCGAGGTGGGCCGCGACACGAAGAGCTTCCA
GGCCGCCCTGCGCGGAGCCCTGCGCGAGGACCCGGACATAATCCTGGTGGGCGAGATGCGCGACCGCGAGACCATCGCCC
TGGCCCTGGAGGCCGCCGAGACAGGCCATCTGGTCTTGTCCACCCTGCATACCGTGTCCGCCGCCAAGACCGTGGACCGC
ATCATCGAGGTGTTTCCCGGCGATACGCAGGGCCAGATCCGCTCCAGCCTGTCCGAGTCGCTGCGCGCCGTGGTCTCGCA
GGTGCTGTTCACCCGCGCGGACAGGCCCGGCCGGGTCGCCGCGCTGGAACTGCTGCTGGCTGTGCCGGCCGTGCGCAACC
TCATTCGCGAGAACAAGACCTACCAGCTGCCTTCGCTCATCCAGATGGGTAAGGCCCAGGGCATGCAGCACCTGGACGAG
CAGATCCAGAAGCTCCTGGACAGCGGCATGATCACCCCCGAGGATGCCCTTGCCCAAGCCGTGGACAAGAAGCGCTTCGC
GCGCCAGGGTGACGGCAGGCTCATGGGCTCCATGTTCAGCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-252)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F3YUY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

53.433

92.798

0.496
  pilT Legionella pneumophila strain Lp02

53.433

92.798

0.496
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.035

95.291

0.496
  pilT Vibrio cholerae strain A1552

52.035

95.291

0.496
  pilT Acinetobacter baylyi ADP1

53.776

91.69

0.493
  pilT Pseudomonas aeruginosa PAK

52.695

92.521

0.488
  pilT Pseudomonas stutzeri DSM 10701

52.695

92.521

0.488
  pilT Acinetobacter baumannii D1279779

52.537

92.798

0.488
  pilT Acinetobacter nosocomialis M2

52.537

92.798

0.488
  pilT Acinetobacter baumannii strain A118

52.537

92.798

0.488
  pilT Neisseria meningitidis 8013

54.517

88.92

0.485
  pilT Neisseria gonorrhoeae MS11

54.206

88.92

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

95.568

0.465
  pilU Pseudomonas stutzeri DSM 10701

42.458

99.169

0.421
  pilU Acinetobacter baylyi ADP1

40.653

93.352

0.38
  pilU Vibrio cholerae strain A1552

39.169

93.352

0.366

Multiple sequence alignment