Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLOCL_RS06835 Genome accession   NC_016627
Coordinates   1601997..1603052 (+) Length   351 a.a.
NCBI ID   WP_014254656.1    Uniprot ID   -
Organism   Acetivibrio clariflavus DSM 19732     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1596997..1608052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLOCL_RS06820 (Clocl_1388) dpsA 1597557..1598429 (+) 873 WP_014254653.1 dipicolinate synthase subunit DpsA -
  CLOCL_RS06825 (Clocl_1389) - 1598433..1599017 (+) 585 WP_014254654.1 dipicolinate synthase subunit B -
  CLOCL_RS06830 (Clocl_1390) - 1599607..1601979 (+) 2373 WP_014254655.1 type II/IV secretion system protein -
  CLOCL_RS06835 (Clocl_1391) pilT 1601997..1603052 (+) 1056 WP_014254656.1 type IV pilus twitching motility protein PilT Machinery gene
  CLOCL_RS06840 (Clocl_1392) - 1603415..1604629 (+) 1215 WP_014254657.1 type II secretion system F family protein -
  CLOCL_RS06845 (Clocl_1393) - 1604770..1605288 (+) 519 WP_014254658.1 type II secretion system protein -
  CLOCL_RS06850 (Clocl_1394) - 1605351..1605965 (+) 615 WP_014254659.1 PulJ/GspJ family protein -
  CLOCL_RS06855 (Clocl_1395) - 1605989..1606546 (+) 558 WP_014254660.1 PilN domain-containing protein -
  CLOCL_RS06860 (Clocl_1396) - 1606550..1607716 (+) 1167 WP_014254661.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39123.09 Da        Isoelectric Point: 6.4933

>NTDB_id=43170 CLOCL_RS06835 WP_014254656.1 1601997..1603052(+) (pilT) [Acetivibrio clariflavus DSM 19732]
MDLNAILTKAVNAGASDLHICAGVPPVIRLHGDLINLDEPILTPHDCVELAKQCLNSRLYETFLEEGEVDSSYSLPGVAR
FRVNVFKQRGTCAIAFRTIPQEIPKIEDLGLPGLVYDLSLKQRGLILITGPTGHGKSTTLAAMINYINSKRRCHIVTIED
PIEFLHRHNKSVINQREIGADTKNYANSLRAVLREDPDVILIGEMRDQETIATALTAAETGHLVLSTLHTIGAAKTIDRI
IDVFPPYQQAQIRTQLSTVLQGIISQQLIRRADNKGRVLATETMIWTPAIANLIRESRTHQINTCIQTGSQFGMYTMDMC
LAELYKQELIDYESACQYSVDMDNLRKLISM

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=43170 CLOCL_RS06835 WP_014254656.1 1601997..1603052(+) (pilT) [Acetivibrio clariflavus DSM 19732]
ATGGATCTTAATGCGATTCTGACAAAAGCGGTAAATGCGGGCGCATCAGACCTTCATATTTGTGCGGGTGTACCACCGGT
AATCAGACTACATGGTGATTTGATTAATCTTGATGAGCCAATACTTACACCCCATGACTGTGTAGAATTGGCAAAACAGT
GTTTGAACAGCAGATTATATGAAACCTTTTTGGAAGAGGGAGAAGTGGATTCGTCCTACTCTCTTCCAGGAGTGGCACGT
TTCAGGGTAAACGTGTTTAAACAGAGAGGTACGTGTGCAATAGCTTTTAGAACAATTCCTCAAGAAATACCTAAAATTGA
GGATTTAGGCTTGCCGGGGTTAGTTTATGATCTTTCTTTGAAGCAAAGAGGTCTTATACTTATAACAGGACCTACAGGAC
ATGGTAAATCCACAACTCTTGCTGCTATGATAAATTACATAAACAGTAAGAGAAGATGTCATATTGTGACAATTGAAGAT
CCTATAGAGTTTCTTCACAGACATAATAAGAGTGTTATAAATCAAAGAGAAATAGGTGCAGATACCAAGAACTATGCCAA
TTCATTGAGGGCTGTACTGAGAGAAGACCCTGATGTTATTCTAATAGGTGAGATGCGTGACCAGGAAACCATAGCCACAG
CATTAACTGCTGCCGAGACCGGTCACCTTGTACTGTCAACCTTACATACTATAGGTGCTGCAAAGACTATAGACAGAATT
ATTGACGTTTTCCCACCTTACCAGCAGGCTCAGATAAGGACTCAGCTTTCGACAGTTTTGCAGGGAATTATATCTCAACA
GTTGATAAGACGTGCTGATAACAAAGGTCGTGTTCTTGCTACAGAAACTATGATTTGGACACCTGCTATAGCCAATTTGA
TTCGTGAATCTCGTACTCACCAAATCAATACATGTATACAAACCGGATCTCAATTTGGAATGTATACCATGGATATGTGT
CTGGCGGAACTTTATAAACAAGAGCTTATCGATTATGAAAGTGCATGTCAGTATTCGGTTGACATGGATAATTTAAGAAA
ATTAATTTCAATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.343

95.442

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.138

99.145

0.487
  pilT Vibrio cholerae strain A1552

49.854

97.721

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.854

97.721

0.487
  pilT Neisseria meningitidis 8013

48.844

98.575

0.481
  pilT Pseudomonas stutzeri DSM 10701

48.696

98.291

0.479
  pilT Neisseria gonorrhoeae MS11

48.555

98.575

0.479
  pilT Acinetobacter baumannii D1279779

50.149

95.442

0.479
  pilT Acinetobacter baumannii strain A118

50.149

95.442

0.479
  pilT Acinetobacter nosocomialis M2

50.149

95.442

0.479
  pilT Acinetobacter baylyi ADP1

49.851

95.442

0.476
  pilT Legionella pneumophila strain ERS1305867

48.68

97.151

0.473
  pilT Legionella pneumophila strain Lp02

48.68

97.151

0.473
  pilU Pseudomonas stutzeri DSM 10701

41.311

100

0.413
  pilU Acinetobacter baylyi ADP1

38.352

100

0.385
  pilU Vibrio cholerae strain A1552

37.714

99.715

0.376

Multiple sequence alignment