Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HAP28_RS04735 Genome accession   NZ_CP050009
Coordinates   969593..970573 (+) Length   326 a.a.
NCBI ID   WP_061070563.1    Uniprot ID   A0AAW9M4G5
Organism   Citrobacter sp. Y3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 964593..975573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAP28_RS04705 (HAP28_04705) - 965230..966237 (+) 1008 WP_109739853.1 DUF1202 family protein -
  HAP28_RS04710 (HAP28_04710) hemW 966269..967405 (-) 1137 WP_042998064.1 radical SAM family heme chaperone HemW -
  HAP28_RS04715 (HAP28_04715) - 967398..967991 (-) 594 WP_044263976.1 XTP/dITP diphosphatase -
  HAP28_RS04720 (HAP28_04720) yggU 967999..968289 (-) 291 WP_044255664.1 DUF167 family protein YggU -
  HAP28_RS04725 (HAP28_04725) - 968286..968852 (-) 567 WP_042998061.1 YggT family protein -
  HAP28_RS04730 (HAP28_04730) - 968871..969575 (-) 705 WP_061070564.1 YggS family pyridoxal phosphate-dependent enzyme -
  HAP28_RS04735 (HAP28_04735) pilT 969593..970573 (+) 981 WP_061070563.1 type IV pilus twitching motility protein PilT Machinery gene
  HAP28_RS04740 (HAP28_04740) ruvX 970587..971003 (-) 417 WP_109739854.1 Holliday junction resolvase RuvX -
  HAP28_RS04745 (HAP28_04745) - 971003..971566 (-) 564 WP_042998057.1 YqgE/AlgH family protein -
  HAP28_RS04750 (HAP28_04750) gshB 971741..972688 (-) 948 WP_044255655.1 glutathione synthase -
  HAP28_RS04755 (HAP28_04755) rsmE 972701..973432 (-) 732 WP_042998055.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HAP28_RS04760 (HAP28_04760) endA 973512..974219 (-) 708 WP_044263980.1 deoxyribonuclease I -
  HAP28_RS04765 (HAP28_04765) - 974311..974811 (-) 501 WP_044255652.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35833.90 Da        Isoelectric Point: 7.0211

>NTDB_id=429206 HAP28_RS04735 WP_061070563.1 969593..970573(+) (pilT) [Citrobacter sp. Y3]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMERAPFTATDITGLLADWLDEGQQAALRQNGQLDFAVSLADNQRLRA
SAFHQRQGTSLALRLLPTQCPTLETLGTPSVLPELLRSENGLILVTGATGSGKSTTLAAMVTWLNQHIDGHILTLEDPIE
YIYTSQRCLIQQREAGLHCASFATGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLEPDKQEGRVALYELLINTPAAGNLIREGKSHQLPHVIQTGQQMGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=429206 HAP28_RS04735 WP_061070563.1 969593..970573(+) (pilT) [Citrobacter sp. Y3]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCACG
CTGGCGAAAACGGGGACGCATGGAGCGTGCACCCTTTACCGCAACCGACATTACCGGACTGCTAGCGGACTGGCTGGATG
AGGGGCAGCAGGCGGCGTTACGACAAAATGGGCAACTGGATTTCGCGGTTTCGCTCGCGGATAACCAGCGCCTGCGGGCC
AGCGCCTTTCACCAGCGGCAGGGGACGTCGCTGGCGCTACGGCTGCTGCCGACGCAGTGCCCGACGCTGGAAACGCTGGG
GACGCCTTCTGTGCTGCCCGAACTGCTCCGCAGCGAGAATGGTTTGATTCTGGTGACTGGGGCGACAGGCAGCGGAAAAT
CAACTACCCTGGCGGCGATGGTCACGTGGCTGAATCAGCATATCGATGGACATATTCTGACGCTGGAAGATCCCATCGAG
TATATCTACACCAGCCAGCGCTGCCTGATTCAGCAGCGTGAAGCAGGACTGCACTGCGCCTCATTTGCCACCGGTTTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTTTGCTGGGAGAGCTACGCGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACACGCGGTGCCGCGCAGGCGATCGAGCGACTGATTGATTCT
TTTCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCAGGCAGTCTGCGCGCCGTACTGTCGCAGAAACTGGAGCC
CGACAAGCAGGAGGGGCGCGTTGCGCTGTATGAACTGCTGATCAACACGCCGGCAGCGGGGAATTTGATTCGGGAAGGGA
AGTCCCATCAGTTACCCCATGTCATTCAGACCGGGCAGCAGATGGGGATGATGACCTTTGCCCAGAGCCTGCAACAGCGA
CAGGCACAAGGACGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.902

97.239

0.417
  pilU Vibrio cholerae strain A1552

42.581

95.092

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Acinetobacter baylyi ADP1

39.077

99.693

0.39