Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G7098_RS16565 Genome accession   NZ_CP049980
Coordinates   3505817..3506794 (-) Length   325 a.a.
NCBI ID   WP_144884710.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain 707804     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3500817..3511794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7098_RS16535 (G7098_16535) endA 3501404..3502111 (+) 708 WP_032613924.1 deoxyribonuclease I -
  G7098_RS16540 (G7098_16540) rsmE 3502223..3502954 (+) 732 WP_032613955.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G7098_RS16545 (G7098_16545) gshB 3502974..3503921 (+) 948 WP_059307231.1 glutathione synthase -
  G7098_RS16550 (G7098_16550) - 3504013..3504573 (+) 561 WP_032613928.1 YqgE/AlgH family protein -
  G7098_RS16555 (G7098_16555) ruvX 3504573..3504989 (+) 417 WP_103792614.1 Holliday junction resolvase RuvX -
  G7098_RS16560 (G7098_16560) - 3505028..3505717 (-) 690 WP_032613957.1 hypothetical protein -
  G7098_RS16565 (G7098_16565) pilT 3505817..3506794 (-) 978 WP_144884710.1 type IV pilus twitching motility protein PilT Machinery gene
  G7098_RS16570 (G7098_16570) - 3506812..3507513 (+) 702 WP_032613935.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7098_RS16575 (G7098_16575) - 3507535..3508101 (+) 567 WP_032613937.1 YggT family protein -
  G7098_RS16580 (G7098_16580) yggU 3508098..3508394 (+) 297 WP_032613939.1 DUF167 family protein YggU -
  G7098_RS16585 (G7098_16585) - 3508398..3508991 (+) 594 WP_059307228.1 XTP/dITP diphosphatase -
  G7098_RS16590 (G7098_16590) hemW 3508984..3510126 (+) 1143 WP_059307227.1 radical SAM family heme chaperone HemW -
  G7098_RS16595 (G7098_16595) - 3510190..3510552 (+) 363 WP_103792619.1 endonuclease domain-containing protein -
  G7098_RS16600 (G7098_16600) - 3511034..3511753 (-) 720 WP_103792620.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35641.79 Da        Isoelectric Point: 6.6307

>NTDB_id=429162 G7098_RS16565 WP_144884710.1 3505817..3506794(-) (pilT) [Leclercia adecarboxylata strain 707804]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRQGKMELAPFPGPDVVVLLKSWLSDEQQGAWCAQGQVDFAVTLESHRLRAS
AFAHTQGCSLALRLLPEQCPQLHSLGTPRVIPELLTRDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNQQGRVALFELLINTPAAANLIREGKTYQLPGVIQTGLQAGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=429162 G7098_RS16565 WP_144884710.1 3505817..3506794(-) (pilT) [Leclercia adecarboxylata strain 707804]
ATGGATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACAAGGAAAGATGGAATTGGCGCCTTTTCCGGGGCCGGATGTGGTGGTGCTGCTGAAAAGCTGGCTCAGCG
ATGAACAACAGGGGGCATGGTGTGCTCAGGGGCAGGTCGATTTTGCCGTCACTCTGGAGAGCCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCAGAACAGTGCCCGCAATTGCATAGTCTGGGTAC
GCCGCGGGTGATCCCCGAGCTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGGGCCACCGGCAGCGGCAAGTCCA
CCACGCTTGCTGCAATGGTCGACTTTCTCAATCACCACAGCGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGCAACGCCTTTGCCGATGCGCTGCGGGC
GGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAACTGCGCGATAGCGAAACGATCCGCCTTGCGCTTACCGCCG
CAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACTCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAGCTGGCCGGCAGTTTGCAGGCGGTGCTGGCACAAAAACTCGAAAGGGA
CAACCAGCAGGGGCGCGTGGCGCTGTTTGAGTTACTTATTAACACCCCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGTGTGATCCAGACCGGGCTGCAGGCAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.492
  pilT Vibrio cholerae strain A1552

48.93

100

0.492
  pilT Legionella pneumophila strain Lp02

48.137

99.077

0.477
  pilT Legionella pneumophila strain ERS1305867

48.137

99.077

0.477
  pilT Pseudomonas stutzeri DSM 10701

48.896

97.538

0.477
  pilT Acinetobacter baylyi ADP1

47.095

100

0.474
  pilT Pseudomonas aeruginosa PAK

48.265

97.538

0.471
  pilT Acinetobacter baumannii D1279779

47.649

98.154

0.468
  pilT Acinetobacter baumannii strain A118

47.649

98.154

0.468
  pilT Acinetobacter nosocomialis M2

47.649

98.154

0.468
  pilT Neisseria meningitidis 8013

47.5

98.462

0.468
  pilT Neisseria gonorrhoeae MS11

47.188

98.462

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.631

96.615

0.422
  pilU Vibrio cholerae strain A1552

40.566

97.846

0.397
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.388
  pilU Acinetobacter baylyi ADP1

36.728

99.692

0.366