Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G8D99_RS11785 Genome accession   NZ_CP049916
Coordinates   2612988..2614025 (+) Length   345 a.a.
NCBI ID   WP_166326145.1    Uniprot ID   A0A6G8S639
Organism   Acinetobacter lanii strain 185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2594553..2657431 2612988..2614025 within 0


Gene organization within MGE regions


Location: 2594553..2657431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8D99_RS11710 (G8D99_11710) - 2595143..2595349 (-) 207 WP_196782889.1 GMC oxidoreductase -
  G8D99_RS11715 (G8D99_11715) - 2595470..2596057 (+) 588 Protein_2273 tyrosine-type recombinase/integrase -
  G8D99_RS11725 (G8D99_11725) glpK 2596645..2598123 (+) 1479 WP_166326112.1 glycerol kinase GlpK -
  G8D99_RS11730 (G8D99_11730) - 2598219..2599745 (-) 1527 WP_166326115.1 DHA2 family efflux MFS transporter permease subunit -
  G8D99_RS11735 (G8D99_11735) - 2599752..2600933 (-) 1182 WP_166326118.1 HlyD family efflux transporter periplasmic adaptor subunit -
  G8D99_RS11740 (G8D99_11740) - 2601246..2601743 (-) 498 WP_166326121.1 thioesterase family protein -
  G8D99_RS11745 (G8D99_11745) hemB 2601851..2602864 (+) 1014 WP_166326124.1 porphobilinogen synthase -
  G8D99_RS11750 (G8D99_11750) thiO 2603031..2604149 (+) 1119 WP_166326127.1 glycine oxidase ThiO -
  G8D99_RS11755 (G8D99_11755) - 2604213..2604659 (-) 447 WP_166326130.1 ABZJ_00895 family protein -
  G8D99_RS11760 (G8D99_11760) - 2605209..2606123 (+) 915 WP_166326133.1 hypothetical protein -
  G8D99_RS11765 (G8D99_11765) - 2606228..2607478 (+) 1251 WP_166326136.1 exonuclease SbcCD subunit D C-terminal domain-containing protein -
  G8D99_RS11770 (G8D99_11770) - 2607488..2611090 (+) 3603 WP_166326139.1 AAA family ATPase -
  G8D99_RS11775 (G8D99_11775) - 2611183..2611956 (-) 774 WP_166326142.1 hypothetical protein -
  G8D99_RS11780 (G8D99_11780) - 2612175..2612861 (-) 687 WP_166327754.1 YggS family pyridoxal phosphate-dependent enzyme -
  G8D99_RS11785 (G8D99_11785) pilT 2612988..2614025 (+) 1038 WP_166326145.1 type IV pilus twitching motility protein PilT Machinery gene
  G8D99_RS11790 (G8D99_11790) pilU 2614142..2615260 (+) 1119 WP_166326148.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G8D99_RS11795 (G8D99_11795) fur 2615324..2615761 (-) 438 WP_166326151.1 ferric iron uptake transcriptional regulator -
  G8D99_RS11800 (G8D99_11800) - 2615874..2616272 (+) 399 WP_166326154.1 outer membrane protein assembly factor BamE -
  G8D99_RS11805 (G8D99_11805) - 2616318..2616680 (-) 363 WP_166326157.1 RnfH family protein -
  G8D99_RS11810 (G8D99_11810) - 2616680..2617762 (-) 1083 WP_227554325.1 hypothetical protein -
  G8D99_RS11815 (G8D99_11815) - 2617943..2618398 (-) 456 WP_166326160.1 bacteriohemerythrin -
  G8D99_RS11820 (G8D99_11820) - 2618651..2619499 (-) 849 WP_166327758.1 class II glutamine amidotransferase -
  G8D99_RS11825 (G8D99_11825) argB 2619686..2620597 (-) 912 WP_166326163.1 acetylglutamate kinase -
  G8D99_RS11830 (G8D99_11830) - 2620632..2622059 (-) 1428 WP_166326166.1 phosphomannomutase/phosphoglucomutase -
  G8D99_RS11835 (G8D99_11835) dut 2622300..2622752 (-) 453 WP_166326169.1 dUTP diphosphatase -
  G8D99_RS11840 (G8D99_11840) - 2622888..2624927 (-) 2040 WP_166326172.1 UvrD-helicase domain-containing protein -
  G8D99_RS11845 (G8D99_11845) - 2625083..2625724 (-) 642 WP_166326175.1 OmpA family protein -
  G8D99_RS11850 (G8D99_11850) - 2625809..2626450 (-) 642 WP_166326178.1 OmpA family protein -
  G8D99_RS11855 (G8D99_11855) - 2626695..2627606 (+) 912 WP_166326181.1 acyltransferase -
  G8D99_RS11860 (G8D99_11860) - 2627686..2628735 (+) 1050 WP_166326185.1 preprotein translocase subunit SecA -
  G8D99_RS11865 (G8D99_11865) minC 2628854..2629573 (+) 720 WP_166326188.1 septum site-determining protein MinC -
  G8D99_RS11870 (G8D99_11870) minD 2629637..2630449 (+) 813 WP_166326191.1 septum site-determining protein MinD -
  G8D99_RS11875 (G8D99_11875) minE 2630453..2630725 (+) 273 WP_166326194.1 cell division topological specificity factor MinE -
  G8D99_RS11880 (G8D99_11880) - 2631076..2631363 (+) 288 WP_166326197.1 PA4642 family protein -
  G8D99_RS11885 (G8D99_11885) - 2631488..2632027 (+) 540 WP_166326200.1 hypothetical protein -
  G8D99_RS11890 (G8D99_11890) - 2632056..2635205 (-) 3150 WP_166326203.1 DNA translocase FtsK -
  G8D99_RS11895 (G8D99_11895) trxB 2635476..2636426 (+) 951 WP_166326206.1 thioredoxin-disulfide reductase -
  G8D99_RS11900 (G8D99_11900) - 2636559..2638109 (+) 1551 WP_166326209.1 class I SAM-dependent methyltransferase -
  G8D99_RS11905 (G8D99_11905) aat 2638175..2638906 (+) 732 WP_166326212.1 leucyl/phenylalanyl-tRNA--protein transferase -
  G8D99_RS11910 (G8D99_11910) - 2638931..2639746 (+) 816 WP_166326215.1 arginyltransferase -
  G8D99_RS11915 (G8D99_11915) rimI 2639788..2640249 (-) 462 WP_166326218.1 ribosomal protein S18-alanine N-acetyltransferase -
  G8D99_RS11920 (G8D99_11920) - 2640432..2641313 (-) 882 WP_166326221.1 metal-dependent hydrolase -
  G8D99_RS11925 (G8D99_11925) tuf 2641542..2642732 (-) 1191 WP_166326224.1 elongation factor Tu -
  G8D99_RS11930 (G8D99_11930) fusA 2642825..2644960 (-) 2136 WP_166326227.1 elongation factor G -
  G8D99_RS11935 (G8D99_11935) rpsG 2645089..2645559 (-) 471 WP_016657334.1 30S ribosomal protein S7 -
  G8D99_RS11940 (G8D99_11940) rpsL 2645736..2646110 (-) 375 WP_004648013.1 30S ribosomal protein S12 -
  G8D99_RS11945 (G8D99_11945) - 2646312..2647763 (-) 1452 WP_166326230.1 EcsC family protein -
  G8D99_RS11950 (G8D99_11950) - 2648053..2649282 (+) 1230 WP_166326233.1 beta-ketoacyl synthase N-terminal-like domain-containing protein -
  G8D99_RS11955 (G8D99_11955) hemJ 2649301..2649753 (+) 453 WP_166326236.1 protoporphyrinogen oxidase HemJ -
  G8D99_RS11960 (G8D99_11960) - 2649835..2651091 (-) 1257 WP_166326239.1 D-amino acid dehydrogenase -
  G8D99_RS11965 (G8D99_11965) - 2651240..2651707 (+) 468 WP_166326242.1 Lrp/AsnC ligand binding domain-containing protein -
  G8D99_RS11970 (G8D99_11970) - 2651772..2652437 (-) 666 WP_166326245.1 HAD-IA family hydrolase -
  G8D99_RS11975 (G8D99_11975) - 2652536..2653303 (+) 768 WP_166326247.1 hydroxymethylpyrimidine/phosphomethylpyrimidine kinase -
  G8D99_RS11980 (G8D99_11980) - 2653349..2653954 (-) 606 WP_406741492.1 nuclease-related domain-containing protein -
  G8D99_RS11985 (G8D99_11985) - 2654062..2654559 (-) 498 WP_227554326.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  G8D99_RS11990 (G8D99_11990) sugE 2654705..2655022 (-) 318 WP_166326249.1 quaternary ammonium compound efflux SMR transporter SugE -
  G8D99_RS11995 (G8D99_11995) sugE 2655115..2655432 (-) 318 WP_166326251.1 quaternary ammonium compound efflux SMR transporter SugE -
  G8D99_RS12000 (G8D99_12000) - 2655724..2656728 (-) 1005 WP_166326253.1 copper resistance protein B -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38330.02 Da        Isoelectric Point: 6.5054

>NTDB_id=428642 G8D99_RS11785 WP_166326145.1 2612988..2614025(+) (pilT) [Acinetobacter lanii strain 185]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLIYDIMNDKQRRDFEEQLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGQIFKDICEYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KSLVSKGIISPQIARTAAKQPEAFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=428642 G8D99_RS11785 WP_166326145.1 2612988..2614025(+) (pilT) [Acinetobacter lanii strain 185]
ATGGACATTACAGAGTTATTGGCGTTTTCAGTCAAAAATGGGGCATCGGATTTACACTTATCGGCGGGCATGCCACCGAT
GATTCGTGTCGATGGTGAAGTACGTCGCATTAACTTGCCGGCCTTAGAACATAAAGATGTGCATCGTTTGATTTATGACA
TCATGAACGATAAGCAACGCCGTGATTTTGAAGAACAACTTGAAACTGACTTCTCATTTGAAGTGCCCGGCGTGGCACGT
TTCCGTGTCAATGCCTTTAACCAAAACCGTGGTGCAGGCGCGGTATTCCGTACCATTCCATCGAAAGTATTATCGATGGA
TGATTTGGGCATGGGACAAATCTTCAAAGACATCTGTGAATACCCGCGTGGTATTGTTCTCGTCACTGGCCCTACAGGTT
CGGGTAAATCGACCACCCTTGCTGCGATGATGGACTATATCAACGAAAATCGTTATGACCACATTTTAACCGTTGAAGAC
CCGATCGAGTTTGTACATCAATCAAAAAAATGCTTGATCAACCAACGTGAAGTGCATCGTGATACGCACGGCTTTAATGA
AGCACTTCGTTCTGCGCTACGTGAAGATCCGGACATTATCTTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGCTTGG
CGCTAACTGCTGCGGAAACCGGTCACTTGGTCTTTGGTACGCTACATACCACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTGTTCCCTGCTGAAGAAAAAGACATGGTCCGTGCCATGTTGTCTGAATCGCTTCAAGCGGTGATTTCGCAAAC
GCTACTCAAGAAAAATGGCGGTGGTCGAGTGGCGGCACACGAAATCATGATTGGTATTCCTGCGATTCGAAATTTAATCC
GTGAGAACAAAGTGGCACAGATGTATTCTGCCATCCAAACCGGTGCCAACTATGGCATGACCACATTGGATCAGAGTTTA
AAATCACTGGTCTCTAAAGGCATTATTAGTCCTCAGATTGCACGTACGGCGGCGAAGCAACCTGAAGCATTTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8S639

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.652

100

0.957
  pilT Acinetobacter baumannii D1279779

95.362

100

0.954
  pilT Acinetobacter baumannii strain A118

95.362

100

0.954
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.181

96.232

0.675
  pilT Vibrio cholerae strain A1552

70.181

96.232

0.675
  pilT Neisseria meningitidis 8013

67.536

100

0.675
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.003

98.261

0.403
  pilU Acinetobacter baylyi ADP1

39.942

99.42

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.181

95.942

0.386