Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DESOR_RS04940 Genome accession   NC_016584
Coordinates   1030791..1031837 (+) Length   348 a.a.
NCBI ID   WP_014183508.1    Uniprot ID   G7W840
Organism   Desulfosporosinus orientis DSM 765     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1025791..1036837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESOR_RS04910 (Desor_1005) aroB 1026080..1027171 (+) 1092 WP_014183502.1 3-dehydroquinate synthase -
  DESOR_RS04915 (Desor_1006) - 1027337..1027753 (+) 417 WP_148265227.1 type II secretion system protein GspG -
  DESOR_RS04920 (Desor_1007) - 1027757..1028179 (+) 423 WP_242832455.1 hypothetical protein -
  DESOR_RS04925 (Desor_1008) - 1028166..1028675 (+) 510 WP_014183505.1 type II secretion system protein -
  DESOR_RS04930 (Desor_1009) - 1028693..1029079 (+) 387 WP_042330825.1 hypothetical protein -
  DESOR_RS04935 (Desor_1010) - 1029085..1030773 (+) 1689 WP_042330827.1 GspE/PulE family protein -
  DESOR_RS04940 (Desor_1011) pilT 1030791..1031837 (+) 1047 WP_014183508.1 type IV pilus twitching motility protein PilT Machinery gene
  DESOR_RS04945 (Desor_1012) - 1031834..1033036 (+) 1203 WP_014183509.1 type II secretion system F family protein -
  DESOR_RS04950 (Desor_1013) - 1033139..1033624 (+) 486 WP_014183510.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DESOR_RS04955 (Desor_1014) - 1033693..1034466 (+) 774 WP_042330830.1 A24 family peptidase -
  DESOR_RS27280 - 1034483..1034794 (+) 312 WP_052304274.1 hypothetical protein -
  DESOR_RS04960 (Desor_1015) - 1034866..1035882 (+) 1017 WP_148265228.1 hypothetical protein -
  DESOR_RS04965 (Desor_1016) - 1035979..1036521 (+) 543 WP_014183512.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38425.37 Da        Isoelectric Point: 8.1520

>NTDB_id=42847 DESOR_RS04940 WP_014183508.1 1030791..1031837(+) (pilT) [Desulfosporosinus orientis DSM 765]
MASLQELFHLAGEKKASDIHLTVESPPVLRINGSLLQNKMEKLTQADLNHYANSLMNENQAKRFAEFGELDMSYSLPGVG
RYRVNIFRQRGSIGIVIRLIPFVIPSPEALGLPPVSIELAKLHKGLVLVTGPTGSGKSTTLASLIDYINQTRACHIMTIE
DPIEYLHRHKLSLVNQREVGNDTYSFTNALRAVLRQDPDVVLVGEMRDLETIATAITAAETGHLVFSTLHTNDATQSVDR
MIDVFPPHQQQQIRVQLAAVLEGIMSQQLFPRADGKGRVAAIEVMAATPAVRSLIREGKTHQLPTVIQTNAKLGMQTMDK
AIMDLVKKGLVAYDVAQEKLQSPDSLRR

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=42847 DESOR_RS04940 WP_014183508.1 1030791..1031837(+) (pilT) [Desulfosporosinus orientis DSM 765]
ATGGCTTCATTACAAGAACTTTTTCACTTAGCCGGAGAAAAAAAAGCCTCTGATATACATTTAACGGTTGAGAGCCCTCC
TGTTTTACGTATTAATGGATCTTTGCTTCAGAACAAGATGGAAAAACTTACTCAGGCAGATTTAAACCATTATGCCAATT
CTCTGATGAATGAGAATCAAGCTAAACGATTTGCTGAGTTCGGCGAGTTGGATATGTCCTATAGTTTGCCGGGTGTCGGG
CGCTATCGGGTTAACATCTTCCGGCAGAGAGGGTCTATTGGCATTGTCATTCGTTTAATTCCTTTTGTCATCCCCAGTCC
GGAAGCCTTAGGACTTCCTCCTGTAAGTATAGAACTAGCCAAACTCCATAAAGGATTGGTTTTGGTGACTGGCCCGACTG
GGAGCGGTAAATCGACAACCTTAGCTTCTCTAATTGATTACATCAATCAAACTCGAGCTTGTCATATCATGACAATTGAA
GACCCCATTGAATATTTGCACAGACATAAACTGAGCTTAGTTAATCAGCGAGAGGTTGGCAACGATACTTATTCCTTTAC
GAACGCCTTGCGGGCTGTATTGAGACAAGATCCGGATGTCGTTTTAGTGGGAGAAATGCGGGATTTAGAAACCATCGCGA
CAGCTATCACTGCTGCTGAGACGGGGCACTTGGTCTTTAGCACCCTGCATACCAACGATGCTACTCAGTCTGTGGATCGT
ATGATCGATGTTTTTCCGCCTCATCAGCAGCAGCAAATCCGTGTGCAGCTGGCGGCAGTTTTAGAAGGAATTATGTCTCA
ACAATTGTTCCCCAGGGCGGATGGCAAAGGGAGAGTGGCCGCTATTGAAGTCATGGCAGCAACCCCGGCAGTCCGGAGCC
TTATAAGGGAAGGGAAAACCCATCAGCTTCCGACAGTTATACAAACTAATGCCAAGTTGGGAATGCAAACTATGGATAAG
GCAATTATGGATTTAGTGAAAAAGGGGCTTGTGGCTTATGATGTTGCCCAAGAAAAGCTGCAGAGTCCCGATAGCTTAAG
AAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7W840

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.203

99.138

0.537
  pilT Pseudomonas aeruginosa PAK

48.397

98.563

0.477
  pilT Pseudomonas stutzeri DSM 10701

48.105

98.563

0.474
  pilT Legionella pneumophila strain ERS1305867

48.955

96.264

0.471
  pilT Legionella pneumophila strain Lp02

48.955

96.264

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.225

97.126

0.468
  pilT Vibrio cholerae strain A1552

48.225

97.126

0.468
  pilT Neisseria meningitidis 8013

47.23

98.563

0.466
  pilT Neisseria gonorrhoeae MS11

46.647

98.563

0.46
  pilT Acinetobacter nosocomialis M2

45.773

98.563

0.451
  pilT Acinetobacter baumannii D1279779

45.773

98.563

0.451
  pilT Acinetobacter baumannii strain A118

45.773

98.563

0.451
  pilT Acinetobacter baylyi ADP1

45.773

98.563

0.451
  pilU Pseudomonas stutzeri DSM 10701

43.314

98.851

0.428
  pilU Acinetobacter baylyi ADP1

41.449

99.138

0.411
  pilU Vibrio cholerae strain A1552

42.296

95.115

0.402

Multiple sequence alignment