Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G7090_RS19410 Genome accession   NZ_CP049786
Coordinates   4063149..4064126 (-) Length   325 a.a.
NCBI ID   WP_166183945.1    Uniprot ID   -
Organism   Leclercia sp. 29361     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4058149..4069126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7090_RS19380 (G7090_19380) endA 4058740..4059447 (+) 708 WP_166183940.1 deoxyribonuclease I -
  G7090_RS19385 (G7090_19385) rsmE 4059561..4060292 (+) 732 WP_106994352.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G7090_RS19390 (G7090_19390) gshB 4060312..4061259 (+) 948 WP_106994351.1 glutathione synthase -
  G7090_RS19395 (G7090_19395) - 4061348..4061908 (+) 561 WP_207630086.1 YqgE/AlgH family protein -
  G7090_RS19400 (G7090_19400) ruvX 4061908..4062324 (+) 417 WP_106994349.1 Holliday junction resolvase RuvX -
  G7090_RS19405 (G7090_19405) - 4062363..4063052 (-) 690 WP_166183942.1 IclR family transcriptional regulator -
  G7090_RS19410 (G7090_19410) pilT 4063149..4064126 (-) 978 WP_166183945.1 type IV pilus twitching motility protein PilT Machinery gene
  G7090_RS19415 (G7090_19415) - 4064144..4064845 (+) 702 WP_106994346.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7090_RS19420 (G7090_19420) - 4064867..4065433 (+) 567 WP_106994345.1 YggT family protein -
  G7090_RS19425 (G7090_19425) yggU 4065430..4065726 (+) 297 WP_106994344.1 DUF167 family protein YggU -
  G7090_RS19430 (G7090_19430) - 4065730..4066323 (+) 594 WP_106994343.1 XTP/dITP diphosphatase -
  G7090_RS19435 (G7090_19435) hemW 4066316..4067458 (+) 1143 WP_166183948.1 radical SAM family heme chaperone HemW -
  G7090_RS19440 (G7090_19440) - 4067522..4067884 (+) 363 WP_166183950.1 endonuclease domain-containing protein -
  G7090_RS19445 (G7090_19445) - 4067959..4068678 (-) 720 WP_106994541.1 DUF2884 domain-containing protein -
  G7090_RS19450 (G7090_19450) - 4068731..4069057 (-) 327 WP_142487055.1 YggL family protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35632.80 Da        Isoelectric Point: 6.7158

>NTDB_id=427816 G7090_RS19410 WP_166183945.1 4063149..4064126(-) (pilT) [Leclercia sp. 29361]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMEFAPFPGPDVVMLLKSWLSDEQQGAWCAQGQVDFAVALESHRLRAS
AFAHTQGCSLALRLLPEQCPQLPTLGTPRAIPELLTSDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCPSFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PADEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTWQLPGVIQTGLQAGMQNFEQSRAERR
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=427816 G7090_RS19410 WP_166183945.1 4063149..4064126(-) (pilT) [Leclercia sp. 29361]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCGCG
ATGGCGACGACGAGGGAAGATGGAGTTCGCCCCTTTTCCGGGGCCGGACGTCGTGATGCTGCTGAAAAGCTGGCTTAGCG
ACGAACAGCAGGGGGCATGGTGCGCTCAGGGGCAGGTGGATTTTGCCGTCGCCCTGGAGAGCCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACACAAGGGTGCTCGCTGGCGCTCCGGCTGCTACCGGAACAGTGCCCGCAGCTGCCTACCCTGGGCAC
GCCGCGGGCGATCCCCGAGCTGCTGACCAGCGATAACGGCCTGATTCTGGTGACCGGGGCCACCGGCAGCGGCAAGTCCA
CCACGCTGGCGGCGATGGTCGACTTTCTCAATCACCACAGCGACGGGCATATCCTGACGCTCGAAGATCCGGTGGAGTTC
ATCCATCAGAGCAAGCGCTGTCTGGTGCAGCAGCGGGAGATTGGCCTGCATTGCCCCTCCTTTGCCGATGCGCTGCGGGC
GGCGTTGCGTGAGGATCCCGATGTGATCCTGCTGGGTGAACTGCGCGACAGCGAGACGATCCGCCTTGCGCTCACTGCCG
CCGAGACCGGGCATCTGGTACTGGCGACTCTGCATACCCGCGGCGCGGCGCAGGCGATAGAGCGGCTGGTAGATGCCTTC
CCGGCCGACGAGAAAGAGCCGGTACGTAAGCAGCTGGCGGGTAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCGAAAGGGA
TAACCAGCAGGGGCGCGTCGCGCTGTTTGAGTTACTGGTTAATACCCCCGCGGCGGCAAACTTAATCCGTGAAGGTAAAA
CCTGGCAACTGCCGGGCGTGATCCAGACCGGGTTGCAGGCCGGCATGCAGAACTTCGAGCAGAGCCGTGCTGAGCGCAGG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.498
  pilT Vibrio cholerae strain A1552

49.541

100

0.498
  pilT Legionella pneumophila strain Lp02

47.516

99.077

0.471
  pilT Legionella pneumophila strain ERS1305867

47.516

99.077

0.471
  pilT Neisseria meningitidis 8013

47.812

98.462

0.471
  pilT Neisseria gonorrhoeae MS11

47.5

98.462

0.468
  pilT Pseudomonas stutzeri DSM 10701

47.634

97.538

0.465
  pilT Acinetobacter baylyi ADP1

46.177

100

0.465
  pilT Acinetobacter baumannii D1279779

46.708

98.154

0.458
  pilT Acinetobacter baumannii strain A118

46.708

98.154

0.458
  pilT Acinetobacter nosocomialis M2

46.708

98.154

0.458
  pilT Pseudomonas aeruginosa PAK

46.688

97.538

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.268

96.615

0.428
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.388
  pilU Acinetobacter baylyi ADP1

37.037

99.692

0.369