Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G8S19_RS03660 Genome accession   NZ_CP049738
Coordinates   747730..748710 (+) Length   326 a.a.
NCBI ID   WP_253686100.1    Uniprot ID   -
Organism   Citrobacter sp. SX206     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742730..753710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8S19_RS03620 (G8S19_03625) hemW 743151..744287 (-) 1137 WP_253686096.1 radical SAM family heme chaperone HemW -
  G8S19_RS03625 (G8S19_03630) - 744280..744873 (-) 594 WP_115642890.1 XTP/dITP diphosphatase -
  G8S19_RS03630 (G8S19_03635) yggU 744881..745171 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  G8S19_RS03635 (G8S19_03640) - 745168..745734 (-) 567 WP_003825417.1 YggT family protein -
  G8S19_RS03640 (G8S19_03645) - 745753..746457 (-) 705 WP_253686098.1 YggS family pyridoxal phosphate-dependent enzyme -
  G8S19_RS03645 (G8S19_03650) - 746475..746600 (+) 126 Protein_715 type IV pili twitching motility protein PilT -
  G8S19_RS03650 (G8S19_03655) - 747117..747536 (+) 420 WP_048213194.1 DUF3828 domain-containing protein -
  G8S19_RS03655 (G8S19_03660) - 747542..747712 (-) 171 Protein_717 YggS family pyridoxal phosphate enzyme -
  G8S19_RS03660 (G8S19_03665) pilT 747730..748710 (+) 981 WP_253686100.1 type IV pilus twitching motility protein PilT Machinery gene
  G8S19_RS03665 (G8S19_03670) ruvX 748707..749123 (-) 417 WP_061068855.1 Holliday junction resolvase RuvX -
  G8S19_RS03670 (G8S19_03675) - 749123..749686 (-) 564 WP_253686102.1 YqgE/AlgH family protein -
  G8S19_RS03675 (G8S19_03680) gshB 749798..750745 (-) 948 WP_003027083.1 glutathione synthase -
  G8S19_RS03680 (G8S19_03685) rsmE 750758..751489 (-) 732 WP_137364356.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G8S19_RS03685 (G8S19_03690) endA 751564..752271 (-) 708 WP_058842381.1 deoxyribonuclease I -
  G8S19_RS03690 (G8S19_03695) - 752366..752863 (-) 498 WP_061068543.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36034.05 Da        Isoelectric Point: 6.1737

>NTDB_id=427438 G8S19_RS03660 WP_253686100.1 747730..748710(+) (pilT) [Citrobacter sp. SX206]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKIENAPFTTPDVENLLMCWLSEQQQVQWREQGQVDFAVTLTDSRRLRA
SAFAHQQGTSLALRLLPLDCPHLDDLQTPEAIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYASQRCLIQQREVGVHCVSFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAASEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKNHQLPGVIQTGQQTGMQTFAQSLQQL
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=427438 G8S19_RS03660 WP_253686100.1 747730..748710(+) (pilT) [Citrobacter sp. SX206]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCAGGCGTGGAAAAATCGAAAACGCGCCATTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAGCAAGGGCAGGTTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGCCCATCAGCAAGGAACGTCACTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTCCA
GACTCCCGAGGCCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGTGCCACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGACACATTCTGACGCTGGAAGATCCTATTGAA
TACCGCTACGCCAGCCAGCGTTGTCTCATTCAACAGCGGGAGGTGGGCGTACACTGCGTCTCTTTCGCCGCCGGTTTGCG
CGGTGCGTTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGCTACACACGCGTGGGGCTGCACAAGCCATTGCGCGGCTGGTGGATTCC
TTTGCTGCATCAGAGAAAGATCCGGTGCGTAATCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAGCTGCTCGTTAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AGAACCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTGCAGCAGCTA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

46.44

99.08

0.46
  pilT Legionella pneumophila strain Lp02

46.44

99.08

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.741

99.387

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.319

99.08

0.39
  pilU Acinetobacter baylyi ADP1

37.117

100

0.371