Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CABTHER_RS08330 Genome accession   NC_016024
Coordinates   2030523..2031692 (+) Length   389 a.a.
NCBI ID   WP_014100181.1    Uniprot ID   G2LDI4
Organism   Chloracidobacterium thermophilum B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2025523..2036692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CABTHER_RS08320 (Cabther_A1695) - 2025634..2028312 (-) 2679 WP_014100179.1 ComEC/Rec2 family competence protein -
  CABTHER_RS08325 (Cabther_A1696) pilB 2028491..2030335 (+) 1845 WP_014100180.1 type IV-A pilus assembly ATPase PilB -
  CABTHER_RS08330 (Cabther_A1697) pilT 2030523..2031692 (+) 1170 WP_014100181.1 type IV pilus twitching motility protein PilT Machinery gene
  CABTHER_RS08335 (Cabther_A1698) - 2031959..2033188 (+) 1230 WP_014100182.1 type II secretion system F family protein -
  CABTHER_RS08340 (Cabther_A1699) - 2033288..2034169 (-) 882 WP_014100183.1 hypothetical protein -
  CABTHER_RS08345 (Cabther_A1700) - 2034290..2034934 (+) 645 WP_014100184.1 zinc ribbon domain-containing protein -
  CABTHER_RS08350 (Cabther_A1701) - 2035015..2035359 (-) 345 WP_014100185.1 hypothetical protein -
  CABTHER_RS08355 (Cabther_A1702) - 2035366..2035626 (-) 261 WP_014100186.1 TM2 domain-containing protein -
  CABTHER_RS08360 (Cabther_A1703) - 2035872..2036285 (-) 414 WP_228374045.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43083.52 Da        Isoelectric Point: 7.8903

>NTDB_id=42508 CABTHER_RS08330 WP_014100181.1 2030523..2031692(+) (pilT) [Chloracidobacterium thermophilum B]
MSSNLVLSDLLRKMIELGGSDLHITTNSPPQVRVHGHLRPLDYPELTPADTKQLAYSVLTDAQKHRFEETLELDFSFGIK
GLSRFRANLFNQRGAVAAVFRAIPYEIRTFEELGLPPVVKKLCEKPRGLILVTGPTGSGKSTTLAAMINKINEDRHEHII
TIEDPIEFLHPNKNCLVNQREVHADTHSFANSLRAALRQDPDVVLIGEMRDLETVETALRIAETGHLTFGTLHTNSAYST
INRIIDIFPAHQQSQIRTQLSLVLEGVLCQSLLPKANGQGRCMALEILVPNSAIRNLIREDKIHQIYGMMQTGQDKFGMQ
TFNQSLLNLYLSRQITLEVAMARSHNPDELQDLINRATSGVGGGAMPPGGRPMPPGGPARPGMPPPVRR

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=42508 CABTHER_RS08330 WP_014100181.1 2030523..2031692(+) (pilT) [Chloracidobacterium thermophilum B]
ATGTCGTCAAACCTCGTGCTGAGCGACCTGCTTCGGAAAATGATCGAGCTGGGCGGCTCAGACCTGCACATCACCACCAA
CTCACCACCTCAGGTCCGCGTTCACGGGCATCTGCGTCCGCTGGACTACCCGGAACTGACACCAGCAGACACCAAGCAGT
TGGCGTATTCCGTATTGACCGACGCCCAGAAGCACCGTTTCGAGGAGACACTCGAACTCGACTTCTCCTTTGGCATCAAG
GGCCTGTCCCGCTTCCGGGCCAACCTCTTCAACCAGCGCGGCGCTGTGGCAGCGGTCTTCCGGGCTATCCCCTACGAAAT
CCGTACCTTTGAGGAGTTGGGCCTGCCGCCGGTGGTCAAGAAGCTGTGTGAAAAACCACGGGGTCTCATCCTCGTCACTG
GGCCGACCGGTTCCGGCAAGTCCACAACCCTCGCGGCCATGATCAACAAGATCAACGAGGACCGGCACGAACACATCATC
ACCATTGAAGACCCCATCGAGTTTCTCCACCCGAACAAGAACTGCCTCGTCAACCAGCGTGAAGTCCACGCCGACACGCA
CAGTTTTGCCAACTCCCTGCGCGCTGCCCTGCGGCAGGACCCTGACGTGGTGCTTATCGGGGAGATGCGTGACCTGGAAA
CGGTTGAAACGGCGCTGCGCATCGCTGAAACCGGGCACCTGACCTTCGGCACGCTTCACACAAACTCAGCCTACTCGACC
ATCAACCGGATCATTGACATCTTTCCAGCCCACCAGCAATCGCAGATTCGGACGCAACTCAGCCTGGTTCTTGAAGGCGT
GCTGTGCCAGTCGCTGCTTCCTAAAGCCAACGGACAGGGACGCTGCATGGCGCTCGAAATCCTTGTTCCAAACTCGGCCA
TCCGCAACCTCATCCGGGAAGACAAAATCCACCAGATTTACGGAATGATGCAAACCGGGCAGGACAAATTCGGGATGCAA
ACCTTCAATCAGTCATTACTCAACTTGTATCTCAGTCGTCAGATTACACTCGAAGTGGCGATGGCACGGTCGCACAATCC
CGATGAGTTACAGGACCTCATCAACCGTGCCACCAGCGGGGTCGGCGGCGGCGCCATGCCGCCCGGTGGACGGCCGATGC
CACCGGGCGGTCCGGCGCGGCCCGGCATGCCTCCCCCTGTTCGCCGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2LDI4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.535

96.144

0.486
  pilT Legionella pneumophila strain ERS1305867

55.263

87.918

0.486
  pilT Legionella pneumophila strain Lp02

55.263

87.918

0.486
  pilT Acinetobacter nosocomialis M2

51.163

88.432

0.452
  pilT Acinetobacter baylyi ADP1

51.163

88.432

0.452
  pilT Acinetobacter baumannii D1279779

51.163

88.432

0.452
  pilT Acinetobacter baumannii strain A118

51.163

88.432

0.452
  pilT Neisseria meningitidis 8013

50.872

88.432

0.45
  pilT Pseudomonas aeruginosa PAK

50.581

88.432

0.447
  pilT Pseudomonas stutzeri DSM 10701

50.291

88.432

0.445
  pilT Neisseria gonorrhoeae MS11

50.291

88.432

0.445
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.038

82.005

0.427
  pilT Vibrio cholerae strain A1552

52.038

82.005

0.427
  pilU Pseudomonas stutzeri DSM 10701

44.348

88.689

0.393
  pilU Vibrio cholerae strain A1552

43.228

89.203

0.386
  pilU Acinetobacter baylyi ADP1

41.21

89.203

0.368

Multiple sequence alignment