Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IEM32_RS24125 Genome accession   NZ_CP062160
Coordinates   4885549..4886529 (-) Length   326 a.a.
NCBI ID   WP_001055622.1    Uniprot ID   -
Organism   Escherichia coli strain 20R2R     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4880549..4891529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEM32_RS24095 (IEM32_24095) yggI 4881197..4881694 (+) 498 WP_001303653.1 SprT family zinc-dependent metalloprotease -
  IEM32_RS24100 (IEM32_24100) endA 4881789..4882496 (+) 708 WP_000286494.1 deoxyribonuclease I -
  IEM32_RS24105 (IEM32_24105) rsmE 4882576..4883307 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IEM32_RS24110 (IEM32_24110) gshB 4883320..4884267 (+) 948 WP_000593272.1 glutathione synthase -
  IEM32_RS24115 (IEM32_24115) yqgE 4884379..4884942 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  IEM32_RS24120 (IEM32_24120) ruvX 4884942..4885358 (+) 417 WP_000017110.1 Holliday junction resolvase RuvX -
  IEM32_RS24125 (IEM32_24125) pilT 4885549..4886529 (-) 981 WP_001055622.1 type IV pilus twitching motility protein PilT Machinery gene
  IEM32_RS24130 (IEM32_24130) yggS 4886547..4887251 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  IEM32_RS24135 (IEM32_24135) yggT 4887269..4887835 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  IEM32_RS24140 (IEM32_24140) yggU 4887832..4888122 (+) 291 WP_001277224.1 DUF167 family protein YggU -
  IEM32_RS24145 (IEM32_24145) rdgB 4888130..4888723 (+) 594 WP_001174762.1 XTP/dITP diphosphatase -
  IEM32_RS24150 (IEM32_24150) hemW 4888716..4889852 (+) 1137 WP_000239959.1 radical SAM family heme chaperone HemW -
  IEM32_RS24155 (IEM32_24155) yggM 4890095..4891102 (-) 1008 WP_000745248.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35982.18 Da        Isoelectric Point: 5.7654

>NTDB_id=423892 IEM32_RS24125 WP_001055622.1 4885549..4886529(-) (pilT) [Escherichia coli strain 20R2R]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLTENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=423892 IEM32_RS24125 WP_001055622.1 4885549..4886529(-) (pilT) [Escherichia coli strain 20R2R]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGACGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CACACCACCGATATTGCCGGAGTTACTCAAGAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTTGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACTGGGCAACAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

37.654

99.387

0.374