Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IF130_RS09855 Genome accession   NZ_CP061840
Coordinates   2200719..2201753 (-) Length   344 a.a.
NCBI ID   WP_011214261.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. pneumophila strain AW-13-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2195719..2206753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF130_RS09835 (IF130_09835) rpoZ 2197626..2197829 (-) 204 WP_011214258.1 DNA-directed RNA polymerase subunit omega -
  IF130_RS09840 (IF130_09840) gmk 2197880..2198509 (-) 630 WP_011214259.1 guanylate kinase -
  IF130_RS09845 (IF130_09845) - 2198515..2199381 (-) 867 WP_011214260.1 YicC/YloC family endoribonuclease -
  IF130_RS09850 (IF130_09850) rph 2199683..2200390 (+) 708 WP_010947728.1 ribonuclease PH -
  IF130_RS09855 (IF130_09855) pilT 2200719..2201753 (-) 1035 WP_011214261.1 type IV pilus twitching motility protein PilT Machinery gene
  IF130_RS09860 (IF130_09860) - 2201917..2202609 (+) 693 WP_011214262.1 YggS family pyridoxal phosphate-dependent enzyme -
  IF130_RS09865 (IF130_09865) proC 2202609..2203397 (+) 789 WP_011214263.1 pyrroline-5-carboxylate reductase -
  IF130_RS09870 (IF130_09870) - 2203741..2204316 (+) 576 WP_011214264.1 YggT family protein -
  IF130_RS09875 (IF130_09875) - 2204376..2204978 (+) 603 WP_224750828.1 DUF2845 domain-containing protein -
  IF130_RS09880 (IF130_09880) - 2204992..2205273 (+) 282 WP_010947734.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38390.26 Da        Isoelectric Point: 7.0290

>NTDB_id=422995 IF130_RS09855 WP_011214261.1 2200719..2201753(-) (pilT) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLAR
FRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPTIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHL
TELVNKNIISKHTAHEAALNKSLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=422995 IF130_RS09855 WP_011214261.1 2200719..2201753(-) (pilT) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
ATGGATATCGCGGAATTGTTGGCATTTTCAGTTAAAAACAAGTCATCAGATTTGCATATTTCAGCTGGCATGCCTCCTAT
GATTCGTGTTGATGGTGATTTACGCAAAATTAATTTACCTCCATTAGAGCATAAAGACGTCATAAAGATTATTTATGACA
TAATGAATGACAGACAAAGAAAAGAATATGAAGAGTTTTTTGAAACAGATTTTTCCTTTGAAATAGCTAATCTGGCACGG
TTCAGGGTTAATGCTTTCAATCAATCACGCGGCGCCGCTGCTGTTTTCAGAACTATTCCCTCTGAAATTCTTAGCATGGA
AGATTTAGGATTACCTACAATTTTTAAAGAAATGGCCAGTTTTTCCAGAGGGCTGGTTCTGGTTACTGGCCCAACTGGAT
CAGGAAAAAGTACCACGCTTGCAGCAGTCATCGATTATATTAATTCAAGTCGTTATGAGCATATTTTGACAGTTGAAGAT
CCCATTGAGTTTGTTCATCAAAGCAAAAAATGTCTGGTTAATCAACGTGAAGTGCACAAAGACACATTGAGCTTTAATGC
CGCTTTGAGATCAGCGCTACGTGAAGATCCTGATATTATATTGGTGGGTGAATTACGTGATTTGGAAACTATACGCTTGG
CAATGACCGCAGCAGAAACCGGGCATTTGGTTTTTGGAACATTGCATACCAATTCTGCAACAAAAACAATAAACAGGATA
ATCGATGTATTTCCCGCTGAAGAAAAATCGATGGTACGCTCTATGCTTTCTGAATCATTACAAGCTGTCGTTGCTCAAAG
TTTATTAAAAAAGAATAAAGGTGGTCGGGTAGCGGCGCTTGAAATTATGATGTGTACCGGGGCTATTCGTAATTTAATTC
GTGAGGATAAGATAGCGCAAATGTATTCGTCAATTCAAACTGGTCAAGCCAAAGGAATGCAAACATTGGATCAACATTTG
ACTGAATTGGTTAATAAAAATATTATTTCGAAACACACTGCTCATGAGGCTGCCCTGAACAAGTCATTATTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

99.709

100

0.997
  pilT Legionella pneumophila strain Lp02

99.709

100

0.997
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Acinetobacter baylyi ADP1

73.256

100

0.733
  pilT Pseudomonas aeruginosa PAK

74.556

98.256

0.733
  pilT Pseudomonas stutzeri DSM 10701

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.477

95.64

0.645
  pilT Vibrio cholerae strain A1552

67.477

95.64

0.645
  pilT Neisseria meningitidis 8013

64.809

99.128

0.642
  pilT Neisseria gonorrhoeae MS11

64.516

99.128

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.494
  pilU Vibrio cholerae strain A1552

42.773

98.547

0.422
  pilU Pseudomonas stutzeri DSM 10701

42.593

94.186

0.401
  pilU Acinetobacter baylyi ADP1

40.06

96.512

0.387