Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G0027_RS12035 Genome accession   NZ_CP048654
Coordinates   2496604..2497641 (-) Length   345 a.a.
NCBI ID   WP_180192401.1    Uniprot ID   A0A7S6VRN6
Organism   Acinetobacter indicus strain C15_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491604..2502641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0027_RS12010 (G0027_11990) - 2492669..2492992 (+) 324 WP_005179774.1 RnfH family protein -
  G0027_RS12015 (G0027_11995) - 2493025..2494329 (-) 1305 WP_152342363.1 IS4 family transposase -
  G0027_RS12020 (G0027_12000) - 2494437..2494835 (-) 399 WP_005179773.1 outer membrane protein assembly factor BamE -
  G0027_RS12025 (G0027_12005) fur 2494948..2495385 (+) 438 WP_005179771.1 ferric iron uptake transcriptional regulator -
  G0027_RS12030 (G0027_12010) pilU 2495458..2496576 (-) 1119 WP_005179770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G0027_RS12035 (G0027_12015) pilT 2496604..2497641 (-) 1038 WP_180192401.1 type IV pilus twitching motility protein PilT Machinery gene
  G0027_RS12040 (G0027_12020) - 2497765..2498451 (+) 687 WP_180192402.1 YggS family pyridoxal phosphate-dependent enzyme -
  G0027_RS12045 (G0027_12025) - 2498497..2499594 (-) 1098 WP_180192403.1 acyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38276.82 Da        Isoelectric Point: 6.4194

>NTDB_id=422138 G0027_RS12035 WP_180192401.1 2496604..2497641(-) (pilT) [Acinetobacter indicus strain C15_T]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLGMGQIFKDICEYPRGLVLVTGPTGSGKSTTLAAMIDFINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQSLLKKNGGGRVAAYEIMIGIPAVRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVSRGVISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=422138 G0027_RS12035 WP_180192401.1 2496604..2497641(-) (pilT) [Acinetobacter indicus strain C15_T]
ATGGATATTACAGAACTATTGGCCTTTTCGGCAAAAAATGGGGCATCGGATTTACACCTGTCGGCAGGCATGCCGCCACT
GATTCGTGTGGATGGTGAAGTACGCCGCATCAATTTGCCCGCCTTGGAACATAAAGAAGTACACAAATTAGTGTACGACA
TTATGAATGATAAACAACGTCGTGATTATGAAGAAAATCTAGAAACGGACTTTTCTTTTGAAGTCCCAGGCGTGGCTCGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCAGGTGCAGTATTCCGTACCATTCCTTCTAAAGTTTTGACCCTTGA
AGATTTGGGAATGGGACAAATCTTTAAAGATATTTGTGAATATCCACGTGGTTTGGTATTGGTGACAGGCCCAACGGGTT
CAGGTAAATCAACCACACTTGCCGCGATGATTGACTTTATTAATGACAATCGCTACGACCATATTCTGACCGTCGAAGAC
CCAATCGAATTTGTACACCAATCTAAAAAATGTTTGGTTAACCAACGAGAAGTACACCGTGATACGCATGGCTTTAACGA
AGCATTACGTTCGGCACTGCGTGAAGACCCTGACATTATTCTAGTGGGTGAGATGCGTGACTTAGAAACCATTCGTTTGG
CACTGACTGCTGCTGAAACGGGTCACTTGGTATTTGGTACGCTACACACCACCTCTGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGTTGTCTGAATCTTTACAAGCCGTCATTTCACAATC
CCTGCTCAAGAAAAATGGTGGTGGTCGTGTAGCTGCGTATGAAATCATGATTGGTATTCCTGCAGTGCGTAACCTGATTC
GTGAAAACAAAGTAGCTCAAATGTACTCGGCAATTCAGACTGGTGCCAACTACGGTATGACCACCCTTGACCAGAGTTTG
AAAAACTTGGTATCGCGCGGTGTGATTAGCCCACAAACGGCTCGTACGGTTGCAAAACAGCCAGAATCGTTCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6VRN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.203

100

0.942
  pilT Acinetobacter baumannii D1279779

93.913

100

0.939
  pilT Acinetobacter baumannii strain A118

93.913

100

0.939
  pilT Acinetobacter baylyi ADP1

86.047

99.71

0.858
  pilT Pseudomonas aeruginosa PAK

82.849

99.71

0.826
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

68.406

100

0.684
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.75

97.391

0.67
  pilT Vibrio cholerae strain A1552

68.75

97.391

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.101

0.403
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386

Multiple sequence alignment