Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G3A45_RS03610 Genome accession   NZ_CP048617
Coordinates   655309..656364 (-) Length   351 a.a.
NCBI ID   WP_163234539.1    Uniprot ID   A0A6P1YEC1
Organism   Caloranaerobacter azorensis strain T3-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 650309..661364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3A45_RS03585 (G3A45_03585) - 650383..651099 (-) 717 WP_163234536.1 CPBP family intramembrane glutamic endopeptidase -
  G3A45_RS13395 (G3A45_03590) - 651260..652717 (+) 1458 WP_250160081.1 DDE-type integrase/transposase/recombinase -
  G3A45_RS03595 (G3A45_03595) - 652869..653621 (-) 753 WP_163234537.1 A24 family peptidase -
  G3A45_RS13810 (G3A45_03600) - 653633..654052 (-) 420 WP_284142987.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  G3A45_RS03605 (G3A45_03605) - 654072..655283 (-) 1212 WP_163234538.1 type II secretion system F family protein -
  G3A45_RS03610 (G3A45_03610) pilT 655309..656364 (-) 1056 WP_163234539.1 type IV pilus twitching motility protein PilT Machinery gene
  G3A45_RS03615 (G3A45_03615) gspE 656373..658052 (-) 1680 WP_163234540.1 type II secretion system ATPase GspE -
  G3A45_RS03620 (G3A45_03620) aroE 658115..658987 (-) 873 WP_163234541.1 shikimate dehydrogenase -
  G3A45_RS03625 (G3A45_03625) - 659100..659483 (-) 384 WP_250160184.1 YqeG family HAD IIIA-type phosphatase -
  G3A45_RS13815 - 659592..660092 (-) 501 WP_204260306.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
  G3A45_RS13820 - 660127..660870 (-) 744 WP_204260271.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39226.36 Da        Isoelectric Point: 6.9179

>NTDB_id=421841 G3A45_RS03610 WP_163234539.1 655309..656364(-) (pilT) [Caloranaerobacter azorensis strain T3-1]
MNIYELLKKTIELGASDLHITVGYPPVMRINGELIKHGEETLTPEENIDLVKQILDEDKFKQLEINGEIDTSYSIAGLGR
FRVNIYKQRGTYGMAIRAVSLRIPTIEELGLPEIVRELSRKQRGLILVTGPTGSGKSTTLASMIDCINKERSCHILTLED
PIEYLHKHNKSIINQREIGSDSRNFASALRAALRQDPDVILVGEMRDLETISIAITAAETGHLVLSTLHTIGAAKTIDRI
IDVFPPHQQQQIKIQLSTVLEGIISQQLLPRADTQGRVLALEIMIATPAIRNLIREGKTYQIQTAIQTGMKFGMQTMDNS
LINLFKQGLISRNTALDYAVDREFVSRFIGF

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=421841 G3A45_RS03610 WP_163234539.1 655309..656364(-) (pilT) [Caloranaerobacter azorensis strain T3-1]
ATGAATATTTATGAATTATTGAAAAAGACTATTGAATTGGGTGCTTCAGATTTGCATATTACAGTTGGCTATCCGCCTGT
TATGAGAATTAATGGTGAACTTATAAAGCATGGAGAAGAAACATTGACTCCAGAAGAAAATATTGATCTTGTAAAACAAA
TACTTGACGAAGATAAGTTTAAACAATTAGAAATAAATGGCGAGATAGATACTTCCTATTCAATAGCAGGATTAGGAAGA
TTTAGAGTTAATATATATAAACAAAGGGGTACATATGGAATGGCGATACGTGCAGTATCGCTGAGAATTCCTACAATTGA
AGAACTTGGACTACCTGAGATTGTAAGAGAATTATCAAGGAAGCAAAGGGGTTTGATATTAGTAACGGGACCTACTGGTA
GTGGAAAGTCAACGACATTAGCTTCAATGATAGACTGTATAAATAAAGAAAGAAGTTGTCATATTTTGACATTAGAAGAC
CCTATCGAATATTTACATAAACATAATAAAAGTATTATAAATCAAAGAGAGATTGGCAGCGATTCTAGAAATTTTGCTAG
TGCTTTAAGAGCTGCATTAAGACAGGACCCTGATGTAATATTAGTCGGGGAAATGAGAGATTTAGAGACTATTTCGATTG
CTATTACAGCTGCTGAAACAGGGCACCTTGTGCTTTCGACTCTGCATACGATAGGGGCAGCAAAAACTATTGATAGAATT
ATAGATGTTTTTCCTCCTCATCAGCAGCAACAAATTAAAATACAGTTATCTACTGTATTAGAGGGGATAATATCGCAGCA
ATTGTTGCCTAGAGCAGATACTCAAGGAAGAGTATTAGCATTAGAGATAATGATAGCAACACCTGCAATAAGAAATCTAA
TCAGAGAAGGAAAAACATATCAGATACAGACAGCAATACAAACAGGAATGAAATTTGGTATGCAGACAATGGACAATTCA
CTTATAAATCTATTTAAACAGGGATTAATAAGTAGAAATACGGCATTAGATTATGCAGTTGATAGAGAATTTGTGAGTAG
ATTTATAGGGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1YEC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.023

98.575

0.513
  pilT Acinetobacter baylyi ADP1

51.343

95.442

0.49
  pilT Acinetobacter baumannii strain A118

50.746

95.442

0.484
  pilT Acinetobacter nosocomialis M2

50.746

95.442

0.484
  pilT Acinetobacter baumannii D1279779

50.746

95.442

0.484
  pilT Pseudomonas aeruginosa PAK

50.147

96.581

0.484
  pilT Legionella pneumophila strain ERS1305867

49.415

97.436

0.481
  pilT Legionella pneumophila strain Lp02

49.415

97.436

0.481
  pilT Pseudomonas stutzeri DSM 10701

49.558

96.581

0.479
  pilT Neisseria meningitidis 8013

47.977

98.575

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

94.017

0.473
  pilT Vibrio cholerae strain A1552

50.303

94.017

0.473
  pilT Neisseria gonorrhoeae MS11

47.688

98.575

0.47
  pilU Pseudomonas stutzeri DSM 10701

42.773

96.581

0.413
  pilU Acinetobacter baylyi ADP1

41.449

98.291

0.407
  pilU Vibrio cholerae strain A1552

40.882

96.866

0.396

Multiple sequence alignment