Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CALLA_RS07650 Genome accession   NC_015949
Coordinates   1587589..1588650 (-) Length   353 a.a.
NCBI ID   WP_014042776.1    Uniprot ID   G2PZA9
Organism   Caldicellulosiruptor acetigenus 6A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1582589..1593650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CALLA_RS07615 (Calla_1512) - 1583147..1583704 (-) 558 WP_013432165.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALLA_RS07620 (Calla_1513) - 1583701..1584246 (-) 546 WP_014042772.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALLA_RS07625 (Calla_1514) - 1584227..1584550 (-) 324 WP_416352906.1 late competence development ComFB family protein -
  CALLA_RS07630 (Calla_1515) - 1584538..1585323 (-) 786 WP_014042773.1 prepilin peptidase -
  CALLA_RS07635 (Calla_1516) - 1585393..1585827 (-) 435 WP_014042774.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALLA_RS07640 (Calla_1517) - 1585862..1586284 (-) 423 WP_013432160.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALLA_RS07645 (Calla_1518) - 1586307..1587518 (-) 1212 WP_014042775.1 type II secretion system F family protein -
  CALLA_RS07650 (Calla_1519) pilT 1587589..1588650 (-) 1062 WP_014042776.1 type IV pilus twitching motility protein PilT Machinery gene
  CALLA_RS07655 (Calla_1520) - 1588685..1590400 (-) 1716 WP_014042777.1 GspE/PulE family protein -
  CALLA_RS07660 (Calla_1521) - 1590466..1593501 (-) 3036 WP_014042778.1 response regulator -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39527.77 Da        Isoelectric Point: 6.7096

>NTDB_id=42176 CALLA_RS07650 WP_014042776.1 1587589..1588650(-) (pilT) [Caldicellulosiruptor acetigenus 6A]
MELSINAILTDAFLKEASDIHITPGVPPVFRIHGRLVKADESILTPEMVEEFVREIVSEEQWKVLNEKGEVDFSYSIKGV
SRFRVNVYKQRGTFSIAFRIIPVNIPAFETLGLPPVLKEFTKLNKGLVLVTGPTGSGKSTTLASLIDIINTERDLHIITL
EDPIEYLHKHKKSIVNQREIGADTQSFANALRAALREDPDVILVGEMRDLETIAIALTAAETGHVVFSTLHTVGAAKTID
RIIDVFPPYQQLQIRVQLSTVLQGVVSQQLLTRRDGKGRIVATEVMIVNAAIRNLIREGKTFQIQSVIQTHQKTGMITME
QSLLDLYRRGLITREDVFNYATDIEFMQKLLSA

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=42176 CALLA_RS07650 WP_014042776.1 1587589..1588650(-) (pilT) [Caldicellulosiruptor acetigenus 6A]
ATGGAACTAAGTATAAATGCAATTCTGACAGATGCTTTTTTGAAGGAAGCATCGGACATACATATCACACCAGGTGTTCC
ACCTGTGTTTCGTATTCATGGGAGGCTTGTTAAAGCAGATGAATCGATACTAACTCCCGAGATGGTTGAAGAATTTGTCA
GAGAAATTGTTTCGGAAGAACAGTGGAAAGTATTAAATGAAAAAGGGGAAGTTGACTTTTCATATAGTATAAAGGGTGTC
AGTCGTTTTAGGGTTAATGTTTATAAACAGAGAGGCACCTTTTCAATAGCATTTAGAATCATCCCTGTTAACATACCAGC
TTTTGAAACGTTGGGCTTGCCTCCTGTCTTAAAAGAATTTACCAAGCTAAACAAAGGACTTGTATTGGTAACCGGTCCTA
CTGGCTCAGGAAAATCTACAACTCTTGCTTCGCTGATTGACATTATTAATACAGAACGGGACTTGCATATCATAACTTTG
GAAGATCCGATTGAATACTTACACAAGCACAAAAAAAGCATTGTTAATCAGAGGGAAATTGGGGCAGACACCCAAAGTTT
TGCCAATGCTTTGAGAGCAGCATTGAGAGAAGATCCAGATGTTATTCTTGTGGGCGAGATGAGAGATTTAGAAACAATTG
CTATTGCACTTACTGCGGCAGAGACAGGTCACGTGGTCTTTTCTACACTTCACACAGTTGGTGCTGCAAAAACAATAGAT
AGAATCATAGACGTTTTTCCACCCTATCAGCAGCTTCAGATTCGAGTTCAGCTTTCTACAGTACTGCAGGGGGTTGTATC
TCAACAGCTTCTAACCAGAAGGGATGGAAAAGGAAGAATAGTTGCAACCGAAGTAATGATTGTAAATGCAGCTATAAGAA
ACCTTATAAGAGAAGGCAAAACATTCCAAATTCAATCTGTGATTCAGACACATCAAAAAACAGGCATGATTACGATGGAG
CAGTCACTTTTAGATCTTTACAGAAGAGGATTAATTACCAGAGAAGACGTTTTTAATTATGCTACTGACATTGAGTTTAT
GCAGAAGCTATTGAGTGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2PZA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.149

98.584

0.504
  pilT Neisseria meningitidis 8013

50

98.017

0.49
  pilT Legionella pneumophila strain Lp02

51.205

94.051

0.482
  pilT Legionella pneumophila strain ERS1305867

51.205

94.051

0.482
  pilT Neisseria gonorrhoeae MS11

49.275

97.734

0.482
  pilT Vibrio cholerae strain A1552

50.448

94.901

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.448

94.901

0.479
  pilT Acinetobacter baumannii D1279779

50.453

93.768

0.473
  pilT Acinetobacter nosocomialis M2

50.453

93.768

0.473
  pilT Acinetobacter baumannii strain A118

50.453

93.768

0.473
  pilT Acinetobacter baylyi ADP1

50.151

93.768

0.47
  pilT Pseudomonas aeruginosa PAK

49.254

94.901

0.467
  pilT Pseudomonas stutzeri DSM 10701

49.249

94.334

0.465
  pilU Pseudomonas stutzeri DSM 10701

42.229

96.601

0.408
  pilU Vibrio cholerae strain A1552

38

99.15

0.377
  pilU Acinetobacter baylyi ADP1

38.824

96.317

0.374

Multiple sequence alignment