Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BURJV3_RS02550 Genome accession   NC_015947
Coordinates   563272..564399 (-) Length   375 a.a.
NCBI ID   WP_014035879.1    Uniprot ID   A0AAJ1X8S1
Organism   Stenotrophomonas maltophilia JV3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558272..569399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BURJV3_RS02530 (BurJV3_0499) - 558699..559688 (+) 990 WP_014035875.1 fumarylacetoacetate hydrolase family protein -
  BURJV3_RS02535 (BurJV3_0500) maiA 559703..560371 (+) 669 WP_014035876.1 maleylacetoacetate isomerase -
  BURJV3_RS02540 (BurJV3_0501) - 560418..561878 (-) 1461 WP_014035877.1 UdgX family uracil-DNA binding protein -
  BURJV3_RS02545 (BurJV3_0502) - 561859..563142 (-) 1284 WP_014035878.1 putative DNA modification/repair radical SAM protein -
  BURJV3_RS02550 (BurJV3_0503) pilU 563272..564399 (-) 1128 WP_014035879.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BURJV3_RS02555 (BurJV3_0504) - 564521..564886 (+) 366 WP_005415160.1 DUF4398 domain-containing protein -
  BURJV3_RS02560 (BurJV3_0505) - 564883..565767 (+) 885 WP_014035880.1 OmpA family protein -
  BURJV3_RS02565 (BurJV3_0507) - 566092..566328 (+) 237 WP_004154075.1 YdcH family protein -
  BURJV3_RS02570 (BurJV3_0508) - 566630..568000 (+) 1371 WP_014035882.1 pyridoxal-phosphate dependent enzyme -
  BURJV3_RS02575 (BurJV3_0509) - 568124..569296 (+) 1173 WP_014035883.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41410.75 Da        Isoelectric Point: 6.3741

>NTDB_id=42149 BURJV3_RS02550 WP_014035879.1 563272..564399(-) (pilU) [Stenotrophomonas maltophilia JV3]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGIIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=42149 BURJV3_RS02550 WP_014035879.1 563272..564399(-) (pilU) [Stenotrophomonas maltophilia JV3]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCGCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTACTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGCCTCGCTGGAAGAAGGCATGGAAAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGACCCGTACGCCGATTTCTCGGCCGGCGCACCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.765

90.667

0.379
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363

Multiple sequence alignment