Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M655_RS23820 Genome accession   NZ_CP048274
Coordinates   4882793..4883836 (-) Length   347 a.a.
NCBI ID   WP_026558188.1    Uniprot ID   A0A9X7T261
Organism   Brevibacillus sp. NSP2.1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4877793..4888836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M655_RS23795 (M655_023460) - 4878441..4879100 (-) 660 WP_005830874.1 PilN domain-containing protein -
  M655_RS23800 (M655_023465) pilM 4879107..4880153 (-) 1047 WP_026558191.1 pilus assembly protein PilM -
  M655_RS23805 (M655_023470) - 4880259..4880939 (-) 681 WP_237716602.1 A24 family peptidase -
  M655_RS23810 (M655_023475) - 4881111..4881563 (-) 453 WP_026558190.1 type II secretion system protein -
  M655_RS23815 (M655_023480) - 4881585..4882793 (-) 1209 WP_026558189.1 type II secretion system F family protein -
  M655_RS23820 (M655_023485) pilT 4882793..4883836 (-) 1044 WP_026558188.1 type IV pilus twitching motility protein PilT Machinery gene
  M655_RS23825 (M655_023490) - 4883868..4885526 (-) 1659 WP_005830887.1 ATPase, T2SS/T4P/T4SS family -
  M655_RS23830 (M655_023495) - 4885560..4887143 (-) 1584 WP_026558187.1 VanW family protein -
  M655_RS23835 (M655_023500) - 4887167..4887955 (-) 789 WP_081414778.1 YwiC-like family protein -
  M655_RS23840 (M655_023505) - 4888134..4888652 (+) 519 WP_005830893.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38552.51 Da        Isoelectric Point: 7.2638

>NTDB_id=420516 M655_RS23820 WP_026558188.1 4882793..4883836(-) (pilT) [Brevibacillus sp. NSP2.1]
MSSVDINKLLKYAFEKRASDLHISVGTSPVFRIDGELKRLDLPPLTPEDTESMARTLIQEHMYHKFQEKGELDFSYGLTG
VSRFRVNAYQQRGCTGIVARVIPTSVPTIESLCMPEILRKFSQKPQGLLLVTGPTGSGKSTTLAAMIDYINRTMRKHVIT
LEDPIEYLHKHQLSIVNQREIGFDTKDFASGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVLATLHTSDAPQTI
DRIIDVFPGSQQPQMRTQLASVLVGIISQRLFPKVGGGRTVATEILVNTPAVANLIRSEKVHQIKSILQTSREMGMHTLE
MSIKELIASGTITMEAAQYFLNERVLE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=420516 M655_RS23820 WP_026558188.1 4882793..4883836(-) (pilT) [Brevibacillus sp. NSP2.1]
GTGTCTAGTGTCGATATTAACAAGCTGCTGAAGTACGCCTTTGAAAAAAGAGCATCCGACCTGCACATATCGGTAGGGAC
GTCGCCTGTCTTTCGGATTGACGGCGAGCTGAAGCGGCTGGATTTGCCGCCGCTCACTCCGGAAGACACCGAGTCGATGG
CGCGGACCTTGATCCAGGAGCACATGTACCACAAGTTCCAGGAAAAAGGCGAGCTGGATTTCTCTTACGGCCTGACAGGG
GTGTCCCGTTTTCGGGTGAACGCGTATCAGCAGCGCGGCTGCACAGGGATCGTGGCTCGGGTCATTCCGACATCGGTGCC
GACCATCGAATCGCTCTGTATGCCGGAGATTTTGCGGAAATTCTCGCAAAAGCCCCAGGGACTGCTGCTGGTAACAGGTC
CGACAGGCAGCGGGAAATCGACCACGCTTGCCGCGATGATCGACTATATCAACCGCACGATGAGAAAGCACGTGATTACG
TTGGAGGACCCGATCGAGTATTTGCACAAGCATCAGCTCTCCATCGTGAACCAGCGCGAGATCGGCTTTGATACAAAAGA
TTTTGCCAGCGGGCTGCGGGCCGCGCTGCGCCAGGACCCTGACGTGATTCTCGTCGGCGAGATGCGCGATCTGGAGACGA
TCAGCACCGCGATTACGGCGGCGGAGACCGGACATTTGGTGCTCGCGACGCTGCATACGTCAGACGCACCGCAGACCATC
GACCGGATTATTGACGTTTTCCCCGGCAGCCAACAGCCGCAAATGCGGACACAGCTCGCTTCCGTCCTGGTCGGCATCAT
CTCGCAGCGCCTTTTTCCGAAGGTAGGCGGTGGCAGGACGGTGGCGACGGAGATTCTCGTCAATACCCCTGCGGTCGCCA
ACCTGATTCGCAGCGAAAAGGTGCATCAGATCAAGAGCATTCTCCAGACAAGCAGGGAGATGGGCATGCATACGCTGGAG
ATGTCGATCAAGGAGCTGATCGCAAGCGGCACGATCACGATGGAGGCGGCCCAGTATTTCCTGAATGAGAGGGTGCTGGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.488

96.83

0.499
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.488

96.83

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.746

96.542

0.49
  pilT Pseudomonas aeruginosa PAK

50.746

96.542

0.49
  pilT Pseudomonas stutzeri DSM 10701

50.149

96.542

0.484
  pilT Acinetobacter baylyi ADP1

49.552

96.542

0.478
  pilT Acinetobacter baumannii strain A118

49.254

96.542

0.476
  pilT Acinetobacter baumannii D1279779

49.254

96.542

0.476
  pilT Acinetobacter nosocomialis M2

48.955

96.542

0.473
  pilT Neisseria meningitidis 8013

48.214

96.83

0.467
  pilT Neisseria gonorrhoeae MS11

48.214

96.83

0.467
  pilT Legionella pneumophila strain Lp02

45.97

96.542

0.444
  pilT Legionella pneumophila strain ERS1305867

45.97

96.542

0.444
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Vibrio cholerae strain A1552

40

96.542

0.386
  pilU Acinetobacter baylyi ADP1

38.769

93.66

0.363

Multiple sequence alignment