Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynMINOS11_RS00095 Genome accession   NZ_CP047953
Coordinates   21272..22348 (-) Length   358 a.a.
NCBI ID   WP_043736353.1    Uniprot ID   -
Organism   Synechococcus sp. Minos11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16272..27348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynMINOS11_RS00070 (SynMINOS11_00014) - 16813..17268 (+) 456 WP_186548983.1 RNA-binding protein -
  SynMINOS11_RS00075 (SynMINOS11_00015) dusA 17272..18279 (-) 1008 WP_186548985.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynMINOS11_RS00080 (SynMINOS11_00016) msrB 18313..18801 (+) 489 WP_255445424.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynMINOS11_RS00085 (SynMINOS11_00017) - 18794..20050 (+) 1257 WP_186548988.1 NAD(P)/FAD-dependent oxidoreductase -
  SynMINOS11_RS00090 (SynMINOS11_00018) - 20043..21272 (-) 1230 WP_186548990.1 type II secretion system F family protein -
  SynMINOS11_RS00095 (SynMINOS11_00019) pilT 21272..22348 (-) 1077 WP_043736353.1 type IV pilus twitching motility protein PilT Machinery gene
  SynMINOS11_RS00100 (SynMINOS11_00020) pilB/pilB1 22366..23859 (-) 1494 WP_186548992.1 GspE/PulE family protein Machinery gene
  SynMINOS11_RS00105 (SynMINOS11_00021) grpE 23871..24557 (+) 687 WP_186548994.1 nucleotide exchange factor GrpE -
  SynMINOS11_RS00110 (SynMINOS11_00022) dnaJ 24557..25666 (+) 1110 WP_186548996.1 molecular chaperone DnaJ -
  SynMINOS11_RS00115 (SynMINOS11_00023) - 25667..25894 (+) 228 WP_011934441.1 sulfurtransferase TusA family protein -
  SynMINOS11_RS00120 (SynMINOS11_00024) rsgA 25887..26747 (+) 861 WP_186548998.1 ribosome small subunit-dependent GTPase A -
  SynMINOS11_RS00125 (SynMINOS11_00025) - 26749..27084 (-) 336 WP_011934443.1 YbaB/EbfC family nucleoid-associated protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39262.87 Da        Isoelectric Point: 5.9369

>NTDB_id=418708 SynMINOS11_RS00095 WP_043736353.1 21272..22348(-) (pilT) [Synechococcus sp. Minos11]
MELQIETLMEQLVEKGGSDLHLSAGLPPYGRFSGALKPMHEAPLAEESCNRLIFSLLNNNQRKQLEQNWELDCSYGLKGV
ARFRVNVYREKGCYAACLRALGSSIPSLQQLGLPDVVREISERPRGLVLVTGPTGSGKTTTLAALLNHINQQRAEHILTV
EDPIEFVYKSERSVIHQRELGEDTRSFANALKAALREDPDVILVGELRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVAVFAQTLCRRLNPPPGAFGRVMAQEIMVNTPALANLIREGKTAQIYSQLQTGGQYGM
QTLERALANLVDGGQVERGEALSKASKPDELMRLLGEA

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418708 SynMINOS11_RS00095 WP_043736353.1 21272..22348(-) (pilT) [Synechococcus sp. Minos11]
ATGGAGCTGCAGATCGAAACCCTGATGGAGCAATTGGTGGAGAAAGGGGGCAGCGATCTGCACCTCTCCGCCGGATTGCC
GCCTTATGGCCGCTTCAGTGGTGCCTTGAAGCCCATGCATGAAGCGCCGCTGGCCGAGGAAAGCTGCAACCGCTTGATCT
TCAGCCTGCTCAACAACAACCAGCGCAAGCAGCTGGAGCAGAACTGGGAGCTGGACTGCTCCTACGGGCTCAAAGGCGTG
GCCCGCTTTCGGGTCAACGTCTACCGGGAAAAGGGTTGCTATGCCGCCTGCCTGCGGGCCCTGGGCAGCAGCATCCCCAG
CCTTCAGCAACTCGGCCTGCCTGATGTCGTGCGCGAGATCAGCGAGCGGCCGCGGGGGTTGGTGCTGGTGACCGGGCCCA
CGGGCTCAGGCAAAACCACCACCCTGGCGGCGCTGCTAAACCACATCAACCAACAGCGGGCCGAACACATCCTCACGGTG
GAAGACCCGATTGAGTTTGTGTACAAGTCAGAGCGCAGCGTGATTCACCAGCGTGAGCTCGGTGAAGACACCCGCAGCTT
TGCCAATGCCCTCAAGGCCGCCCTGCGGGAAGACCCCGACGTGATCCTGGTGGGGGAACTGCGTGATCTGGAAACCATCC
AGCTCGCCATCACCGCCGCAGAAACAGGCCATTTGGTGTTTGGCACCCTGCACACCAGCTCAGCAGCGCAAACGGTGGAT
CGCATGGTGGATGTGTTTCCACCGGAGCAACAAACCCAGATCCGCGTGCAACTCAGCGGCAGCCTGGTGGCCGTGTTTGC
TCAAACCCTCTGCCGCCGGCTCAATCCACCTCCCGGCGCCTTTGGCCGCGTGATGGCCCAAGAAATCATGGTCAACACCC
CGGCCCTGGCGAACTTGATTCGCGAAGGCAAAACGGCCCAGATCTATTCGCAGCTGCAAACCGGCGGCCAATACGGCATG
CAAACCCTCGAGCGCGCCCTGGCCAACCTGGTGGATGGCGGCCAAGTGGAGCGGGGTGAAGCCTTGAGCAAAGCCAGCAA
ACCCGATGAACTAATGCGGTTGCTCGGTGAGGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.289

97.486

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.895

95.81

0.497
  pilT Vibrio cholerae strain A1552

51.895

95.81

0.497
  pilT Pseudomonas stutzeri DSM 10701

50.716

97.486

0.494
  pilT Acinetobacter baylyi ADP1

49.857

97.486

0.486
  pilT Acinetobacter baumannii strain A118

49.714

97.765

0.486
  pilT Acinetobacter baumannii D1279779

49.714

97.765

0.486
  pilT Acinetobacter nosocomialis M2

49.714

97.765

0.486
  pilT Legionella pneumophila strain Lp02

51.327

94.693

0.486
  pilT Legionella pneumophila strain ERS1305867

51.327

94.693

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.596

99.441

0.483
  pilT Neisseria meningitidis 8013

51.497

93.296

0.48
  pilT Neisseria gonorrhoeae MS11

51.497

93.296

0.48
  pilU Pseudomonas stutzeri DSM 10701

38.395

97.486

0.374
  pilU Vibrio cholerae strain A1552

36.544

98.603

0.36

Multiple sequence alignment