Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynMINOS11_RS00100 Genome accession   NZ_CP047953
Coordinates   22366..23859 (-) Length   497 a.a.
NCBI ID   WP_186548992.1    Uniprot ID   -
Organism   Synechococcus sp. Minos11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17366..28859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynMINOS11_RS00080 (SynMINOS11_00016) msrB 18313..18801 (+) 489 WP_255445424.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynMINOS11_RS00085 (SynMINOS11_00017) - 18794..20050 (+) 1257 WP_186548988.1 NAD(P)/FAD-dependent oxidoreductase -
  SynMINOS11_RS00090 (SynMINOS11_00018) - 20043..21272 (-) 1230 WP_186548990.1 type II secretion system F family protein -
  SynMINOS11_RS00095 (SynMINOS11_00019) pilT 21272..22348 (-) 1077 WP_043736353.1 type IV pilus twitching motility protein PilT Machinery gene
  SynMINOS11_RS00100 (SynMINOS11_00020) pilB/pilB1 22366..23859 (-) 1494 WP_186548992.1 GspE/PulE family protein Machinery gene
  SynMINOS11_RS00105 (SynMINOS11_00021) grpE 23871..24557 (+) 687 WP_186548994.1 nucleotide exchange factor GrpE -
  SynMINOS11_RS00110 (SynMINOS11_00022) dnaJ 24557..25666 (+) 1110 WP_186548996.1 molecular chaperone DnaJ -
  SynMINOS11_RS00115 (SynMINOS11_00023) - 25667..25894 (+) 228 WP_011934441.1 sulfurtransferase TusA family protein -
  SynMINOS11_RS00120 (SynMINOS11_00024) rsgA 25887..26747 (+) 861 WP_186548998.1 ribosome small subunit-dependent GTPase A -
  SynMINOS11_RS00125 (SynMINOS11_00025) - 26749..27084 (-) 336 WP_011934443.1 YbaB/EbfC family nucleoid-associated protein -
  SynMINOS11_RS00130 (SynMINOS11_00026) murB 27109..28026 (-) 918 WP_186549000.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54328.72 Da        Isoelectric Point: 5.6654

>NTDB_id=418709 SynMINOS11_RS00100 WP_186548992.1 22366..23859(-) (pilB/pilB1) [Synechococcus sp. Minos11]
MQSCHVAGRPTPGSRSENPAILKAETVRGAALERFELDESFESFAPPPPPDGPDLVESANDRDTSPVIQLADRILVQALS
RQASDIHIEPQEDGLHVRLRLDGVLQNAFETLPAKVIPALTSRFKIMADLDIAERRQPQDGRIRRRYQGNTVDFRVSVLP
SRFGEKIVLRLLDSSATELGLDLLITDSHALAEVRDMGSKPYGILLVTGPTGSGKSTTLYSLLAERNDPGLNISTVEDPI
EYTLAGITQTQVNREKGVDFAKALRAFMRQDPDVLLVGETRDLETAKTALEAALTGHLVLTTLHCNDAPSAVARLEEMGV
ETYLLSASLIGVLSQRLLRKLCTHCRQPYQPKEATLARFGLEASQNSSISFYQPPANNTCSHCHGLGYKGRIGVYEVMRV
NEVMAEAIGQGAGTDQLRHLALSNGMKTLLGYGLELARQGHTSLEEVERVLLTDSGLEQEIRARNLSTATCVGCGAGLRD
EWLDCPYCLTPRGTLQR

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=418709 SynMINOS11_RS00100 WP_186548992.1 22366..23859(-) (pilB/pilB1) [Synechococcus sp. Minos11]
GTGCAGTCCTGCCATGTTGCTGGGAGGCCGACCCCTGGATCAAGGTCGGAAAACCCAGCCATCCTGAAAGCAGAGACAGT
CCGAGGAGCAGCGTTGGAACGCTTCGAACTCGACGAGAGCTTTGAAAGCTTCGCGCCGCCGCCGCCGCCGGATGGGCCGG
ACCTGGTGGAGAGCGCCAACGATCGCGACACCTCGCCGGTGATTCAGCTGGCGGATCGCATCTTGGTGCAAGCCCTCAGC
CGCCAGGCCAGCGACATCCATATCGAACCGCAGGAGGACGGACTGCATGTGCGGCTGCGGCTTGATGGCGTGCTGCAGAA
CGCTTTTGAAACCCTGCCGGCCAAGGTGATTCCGGCACTCACCAGCCGCTTCAAAATCATGGCGGACCTGGACATCGCCG
AGCGCCGCCAACCCCAAGACGGCCGCATTCGCCGGCGGTATCAAGGCAACACCGTTGATTTCCGCGTCAGCGTGCTGCCG
AGCCGCTTCGGCGAAAAAATTGTGCTGCGGCTGCTGGATTCCAGTGCCACCGAACTGGGGCTTGATCTACTGATCACCGA
CTCCCACGCCTTGGCCGAGGTGCGGGACATGGGCAGCAAGCCCTACGGAATCCTGCTCGTCACAGGGCCAACCGGCTCAG
GAAAATCCACCACCCTCTACTCGCTGCTGGCCGAGCGCAACGATCCCGGGCTCAACATCTCCACGGTGGAAGACCCGATC
GAATACACCCTGGCTGGGATCACGCAAACCCAGGTCAACCGCGAAAAGGGGGTGGACTTCGCCAAGGCCCTAAGGGCCTT
CATGCGCCAAGACCCCGATGTTTTGCTGGTTGGGGAAACGCGCGATCTGGAGACGGCCAAAACCGCGCTGGAGGCCGCGC
TCACCGGCCACCTGGTGCTCACAACGTTGCACTGCAACGACGCGCCCAGTGCTGTAGCCCGGCTTGAGGAGATGGGGGTG
GAGACCTACCTGCTGAGCGCCTCACTGATCGGCGTGCTCTCCCAGCGTTTGCTGCGCAAGCTCTGCACCCACTGCCGCCA
GCCCTACCAACCAAAAGAAGCCACCCTGGCCCGCTTTGGTTTGGAGGCCAGCCAAAACAGCTCCATCAGCTTCTATCAGC
CTCCGGCCAACAACACCTGCAGCCATTGCCATGGCCTGGGTTACAAGGGCCGCATCGGCGTGTACGAGGTGATGCGCGTC
AATGAGGTGATGGCGGAGGCCATCGGCCAAGGCGCCGGCACCGACCAGCTGCGCCACCTGGCCCTCAGCAATGGCATGAA
AACGCTGCTGGGCTACGGGCTGGAACTGGCCCGCCAAGGCCACACCAGCCTGGAGGAGGTGGAGCGGGTGCTGCTGACGG
ACTCAGGACTCGAGCAGGAGATCAGGGCCCGCAACCTCAGCACCGCCACCTGCGTGGGCTGCGGGGCCGGACTGCGCGAT
GAATGGCTGGATTGCCCCTATTGCCTAACTCCCAGGGGCACACTGCAGAGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-340)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

61.435

89.738

0.551
  pilF Thermus thermophilus HB27

45.952

84.507

0.388
  pilB Vibrio campbellii strain DS40M4

47.643

81.087

0.386
  pilB Vibrio parahaemolyticus RIMD 2210633

47.395

81.087

0.384
  pilB Vibrio cholerae strain A1552

47.512

80.885

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.907

78.068

0.366

Multiple sequence alignment