Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynPROSU1_RS00095 Genome accession   NZ_CP047951
Coordinates   21616..22692 (-) Length   358 a.a.
NCBI ID   WP_186571009.1    Uniprot ID   -
Organism   Synechococcus sp. PROS-U-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16616..27692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynPROSU1_RS00070 (SynPROSU1_00015) - 17006..17566 (+) 561 WP_186571004.1 RNA-binding protein -
  SynPROSU1_RS00075 (SynPROSU1_00016) dusA 17580..18575 (-) 996 WP_186571005.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynPROSU1_RS00080 (SynPROSU1_00017) msrB 18616..19119 (+) 504 WP_186571006.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynPROSU1_RS00085 (SynPROSU1_00018) - 19091..20344 (+) 1254 WP_186571007.1 NAD(P)/FAD-dependent oxidoreductase -
  SynPROSU1_RS00090 (SynPROSU1_00019) - 20337..21596 (-) 1260 WP_186571008.1 type II secretion system F family protein -
  SynPROSU1_RS00095 (SynPROSU1_00020) pilT 21616..22692 (-) 1077 WP_186571009.1 type IV pilus twitching motility protein PilT Machinery gene
  SynPROSU1_RS00100 (SynPROSU1_00021) - 22703..24547 (-) 1845 WP_186571010.1 GspE/PulE family protein -
  SynPROSU1_RS00105 (SynPROSU1_00022) grpE 24638..25303 (+) 666 WP_186571011.1 nucleotide exchange factor GrpE -
  SynPROSU1_RS00110 (SynPROSU1_00023) dnaJ 25303..26433 (+) 1131 WP_186571012.1 molecular chaperone DnaJ -
  SynPROSU1_RS00115 (SynPROSU1_00024) - 26430..26657 (+) 228 WP_186571013.1 sulfurtransferase TusA family protein -
  SynPROSU1_RS00120 (SynPROSU1_00025) rsgA 26650..27564 (+) 915 WP_186571014.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39286.77 Da        Isoelectric Point: 5.4513

>NTDB_id=418687 SynPROSU1_RS00095 WP_186571009.1 21616..22692(-) (pilT) [Synechococcus sp. PROS-U-1]
MDLMIEDLMEQLVQGGGSDLHLATGQPPYGRFSGELRPMTERPLAEEDCNKLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGNKIPSVELLDLPPVVLETSARPRGLVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYQSDKSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAVSAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVAVFSQTLCRRQNPAEGQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGEQGM
QTLEKALANLVLNGDVSRAEAMAKASKPGELQRLIGDI

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418687 SynPROSU1_RS00095 WP_186571009.1 21616..22692(-) (pilT) [Synechococcus sp. PROS-U-1]
ATGGATCTGATGATCGAAGACCTGATGGAACAACTGGTGCAGGGCGGTGGCAGTGACCTGCACCTCGCCACCGGTCAGCC
GCCCTATGGCCGCTTCAGCGGTGAGCTGCGTCCGATGACGGAACGGCCACTTGCCGAGGAGGACTGCAACAAGCTGATCT
TCTCGATGCTGAACAACAGCCAGCGCAAGACGCTGGAACAGACATGGGAACTGGACTGCGCCTACGGCTTGAAGGGTGTT
GCCCGCTTCCGCGTGAACGTCTACCGCCAGAAAGGAAGCTATGCGGCCTGCCTGCGGGCCCTCGGCAACAAGATTCCCAG
CGTTGAACTACTGGACCTGCCGCCGGTGGTGCTGGAGACGAGCGCGCGGCCCCGAGGGCTCGTCCTGGTGACCGGTCCCA
CAGGATCAGGAAAGACCACCACCCTTGCGGCACTGCTCGATCACATCAACCACACCCGCAGCGAACACATCCTCACGATC
GAAGACCCAATCGAATTCGTGTATCAGAGCGACAAAAGCCTGGTGCACCAACGCCAACTCAACGAAGACACCCGCAGCTT
CGCCAACGCCTTGCGGGCCGCACTGCGGGAAGACCCGGACGTGATCCTGGTGGGTGAAATGAGGGATCTAGAGACCATTC
AATTGGCGGTGAGTGCTGCCGAAACAGGACATCTCGTCTTCGGCACCCTGCACACCAGTTCAGCGGCCCAGACTGTGGAT
CGCATGGTTGACGTCTTCCCACCGGAACAACAGACGCAGATCCGTGTTCAGCTCTCGGGCAGCTTGGTGGCTGTGTTTTC
CCAGACGCTCTGCCGCCGTCAGAACCCAGCAGAGGGACAGTTCGGCCGCGTGATGGCGCAGGAAATCCTGATCAACACAC
CCGCCACCGCCAACCTGATCCGTGAGGGCAAAACGGCGCAGCTCTACTCGCAGATTCAGACGGGCGGAGAACAGGGCATG
CAGACACTGGAAAAAGCCTTGGCCAATCTGGTGCTCAACGGGGATGTGTCCCGTGCCGAAGCCATGGCCAAAGCCAGCAA
ACCTGGCGAATTGCAGCGGTTGATCGGTGACATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.07

96.089

0.52
  pilT Pseudomonas stutzeri DSM 10701

53.198

96.089

0.511
  pilT Acinetobacter baumannii D1279779

51.744

96.089

0.497
  pilT Acinetobacter baumannii strain A118

51.744

96.089

0.497
  pilT Acinetobacter nosocomialis M2

51.744

96.089

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.142

98.603

0.494
  pilT Legionella pneumophila strain Lp02

52.522

94.134

0.494
  pilT Legionella pneumophila strain ERS1305867

52.522

94.134

0.494
  pilT Acinetobacter baylyi ADP1

50.872

96.089

0.489
  pilT Neisseria gonorrhoeae MS11

50.437

95.81

0.483
  pilT Neisseria meningitidis 8013

51.807

92.737

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.588

94.972

0.48
  pilT Vibrio cholerae strain A1552

50.588

94.972

0.48
  pilU Pseudomonas stutzeri DSM 10701

38.462

98.045

0.377
  pilU Vibrio cholerae strain A1552

38.643

94.693

0.366
  pilU Acinetobacter baylyi ADP1

36.963

97.486

0.36

Multiple sequence alignment