Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynROS8604_RS00095 Genome accession   NZ_CP047946
Coordinates   22097..23170 (-) Length   357 a.a.
NCBI ID   WP_186544668.1    Uniprot ID   -
Organism   Synechococcus sp. ROS8604     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17097..28170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynROS8604_RS00070 (SynROS8604_00014) - 17353..17805 (+) 453 WP_186544664.1 RNA-binding protein -
  SynROS8604_RS00075 (SynROS8604_00015) dusA 17824..18828 (-) 1005 WP_186544665.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynROS8604_RS00080 (SynROS8604_00016) msrB 18897..19409 (+) 513 WP_186544666.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynROS8604_RS00085 (SynROS8604_00017) - 19444..20652 (+) 1209 WP_370586594.1 NAD(P)/FAD-dependent oxidoreductase -
  SynROS8604_RS00090 (SynROS8604_00018) - 20627..21901 (-) 1275 WP_186545722.1 type II secretion system F family protein -
  SynROS8604_RS00095 (SynROS8604_00019) pilT 22097..23170 (-) 1074 WP_186544668.1 type IV pilus twitching motility protein PilT Machinery gene
  SynROS8604_RS00100 (SynROS8604_00020) pilB/pilB1 23182..24954 (-) 1773 WP_370586531.1 GspE/PulE family protein Machinery gene
  SynROS8604_RS00105 (SynROS8604_00021) grpE 25034..25759 (+) 726 WP_255445108.1 nucleotide exchange factor GrpE -
  SynROS8604_RS00110 (SynROS8604_00022) dnaJ 25802..26932 (+) 1131 WP_186544671.1 molecular chaperone DnaJ -
  SynROS8604_RS00115 (SynROS8604_00023) - 26936..27178 (+) 243 WP_186544672.1 sulfurtransferase TusA family protein -
  SynROS8604_RS00120 (SynROS8604_00024) rsgA 27165..28079 (+) 915 WP_370586532.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39183.63 Da        Isoelectric Point: 6.1067

>NTDB_id=418646 SynROS8604_RS00095 WP_186544668.1 22097..23170(-) (pilT) [Synechococcus sp. ROS8604]
MEPMIEDLMEQLVNGGGSDLHIASGQPPYGRFSGELRPMQEEALSEEGCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPGVELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRAEHILTI
EDPIEFVYNSDLSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLTAVFSQTLCKRQNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGERGM
QTLERALADLVHKGEISLDEGRSKASKPAELDRLMNN

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=418646 SynROS8604_RS00095 WP_186544668.1 22097..23170(-) (pilT) [Synechococcus sp. ROS8604]
ATGGAACCAATGATCGAAGACCTGATGGAACAACTGGTGAATGGAGGTGGAAGCGACCTCCACATCGCCAGCGGTCAACC
GCCCTATGGCCGATTCAGCGGCGAACTGCGACCGATGCAGGAGGAAGCACTCAGTGAAGAAGGCTGCAACCGCCTGATCT
TCTCCATGCTCAATAACAGCCAACGCAAAACGCTGGAACAAACGTGGGAGCTGGATTGCGCGTATGGATTGAAAGGGGTC
GCCCGTTTTCGCGTGAATGTTTACCGGCAGAAAGGCAGCTACGCCGCCTGCTTGCGTGCTCTTGGAAGCAAAATCCCCGG
CGTTGAATTGCTGAATCTGCCTCCCGTCGTGGTGGAAACCAGCAAACGTCCCCGTGGTCTTGTCCTGGTCACGGGTCCGA
CTGGCTCCGGCAAAACCACCACCCTCGCAGCGCTTCTCGATCACATCAATCACACGAGGGCTGAGCACATCCTCACCATC
GAAGATCCGATCGAATTTGTGTACAACAGTGATCTGAGCTTGGTGCATCAACGCCAACTCAACGAGGACACGCGCAGTTT
TGCCAATGCTCTGCGCGCTGCACTGCGCGAAGACCCAGACGTGATCTTGGTGGGTGAAATGCGCGATCTCGAAACGATTC
AACTAGCGATCAGCGCTGCTGAAACCGGGCATTTGGTGTTTGGGACCCTGCACACCAGTTCCGCGGCGCAGACCGTGGAT
CGGATGGTGGATGTGTTTCCACCTGGCCAGCAAACTCAAATCCGCGTGCAGCTCTCAGGAAGCCTGACTGCTGTGTTCTC
TCAGACACTCTGCAAACGCCAAAATCCTGCCCCTGGGCAGTTCGGTCGCGTGATGGCTCAAGAAATCATGATCAACACTC
CAGCCATCGCCAATTTGATCCGTGAAGGCAAAACAGCTCAGCTGTATTCCCAAATACAAACTGGCGGCGAACGCGGCATG
CAAACCCTTGAGAGAGCCCTTGCCGATTTAGTTCACAAGGGCGAGATCAGCCTTGATGAAGGGCGCAGCAAAGCAAGCAA
ACCCGCTGAACTAGATCGCCTGATGAACAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.36

96.359

0.524
  pilT Pseudomonas stutzeri DSM 10701

54.36

96.359

0.524
  pilT Acinetobacter nosocomialis M2

52.326

96.359

0.504
  pilT Acinetobacter baumannii D1279779

52.326

96.359

0.504
  pilT Acinetobacter baumannii strain A118

52.326

96.359

0.504
  pilT Acinetobacter baylyi ADP1

51.744

96.359

0.499
  pilT Legionella pneumophila strain ERS1305867

52.853

93.277

0.493
  pilT Legionella pneumophila strain Lp02

52.853

93.277

0.493
  pilT Neisseria meningitidis 8013

51.32

95.518

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.292

98.88

0.487
  pilT Neisseria gonorrhoeae MS11

51.026

95.518

0.487
  pilT Vibrio cholerae strain A1552

50.882

95.238

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.882

95.238

0.485
  pilU Pseudomonas stutzeri DSM 10701

38.462

98.319

0.378
  pilU Vibrio cholerae strain A1552

38.905

97.199

0.378

Multiple sequence alignment