Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynROS8604_RS00100 Genome accession   NZ_CP047946
Coordinates   23182..24954 (-) Length   590 a.a.
NCBI ID   WP_370586531.1    Uniprot ID   A0A7G8FYP8
Organism   Synechococcus sp. ROS8604     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18182..29954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynROS8604_RS00080 (SynROS8604_00016) msrB 18897..19409 (+) 513 WP_186544666.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynROS8604_RS00085 (SynROS8604_00017) - 19444..20652 (+) 1209 WP_370586594.1 NAD(P)/FAD-dependent oxidoreductase -
  SynROS8604_RS00090 (SynROS8604_00018) - 20627..21901 (-) 1275 WP_186545722.1 type II secretion system F family protein -
  SynROS8604_RS00095 (SynROS8604_00019) pilT 22097..23170 (-) 1074 WP_186544668.1 type IV pilus twitching motility protein PilT Machinery gene
  SynROS8604_RS00100 (SynROS8604_00020) pilB/pilB1 23182..24954 (-) 1773 WP_370586531.1 GspE/PulE family protein Machinery gene
  SynROS8604_RS00105 (SynROS8604_00021) grpE 25034..25759 (+) 726 WP_255445108.1 nucleotide exchange factor GrpE -
  SynROS8604_RS00110 (SynROS8604_00022) dnaJ 25802..26932 (+) 1131 WP_186544671.1 molecular chaperone DnaJ -
  SynROS8604_RS00115 (SynROS8604_00023) - 26936..27178 (+) 243 WP_186544672.1 sulfurtransferase TusA family protein -
  SynROS8604_RS00120 (SynROS8604_00024) rsgA 27165..28079 (+) 915 WP_370586532.1 ribosome small subunit-dependent GTPase A -
  SynROS8604_RS00125 (SynROS8604_00025) - 28054..28395 (-) 342 WP_006854981.1 YbaB/EbfC family nucleoid-associated protein -
  SynROS8604_RS00130 (SynROS8604_00026) murB 28420..29358 (-) 939 WP_186544673.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 590 a.a.        Molecular weight: 64728.88 Da        Isoelectric Point: 6.0468

>NTDB_id=418647 SynROS8604_RS00100 WP_370586531.1 23182..24954(-) (pilB/pilB1) [Synechococcus sp. ROS8604]
MSADFNRMPLPPALPAKLISEAELAAGEALLQSGKVLDLETWKQLQALPIHLSVDTLMVAIASSSNRENREQLKQILRSH
GYISKLVLANAADLKRILDPQSVDSNSKINQASVSETAKSFLDGFDVEGILNADPDEAEIQSNSASSLDVDPSSTDQSPI
ITLVDRILAKALDMNASDIHVEPQYSGLQIRFRKDGVLTNLTQPLPTKLIPAITSRFKIMADLDIAERRQAQDGRIRRNY
KERTVDFRVNSLPSRYGEKICLRLLDSQSTQLGLDKLISNPTTLELVRTLGSKPFGMILVTGPTGSGKSTTLYSLLAERN
EPGINISTVEDPIEYTLPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGETRDQETAKTAIEAALTGHLVLTTLHCND
APSAIARLDEMGVEPFMVSASLLGIVSQRLLRRVCSDCRIPYHPDSQKLGRFGLLTSHEGDVTFFKAKHHERQQSACPRC
QGTGYKGRIGVYEVLRMNEALAASVAKGATTDLVRQLALEAGMKTLLGYSLDLVREGHTTLEEVDRMVLTDAGLESEQRA
RALSTITCRGCGGGLQEGWLECLYCLTPRQ

Nucleotide


Download         Length: 1773 bp        

>NTDB_id=418647 SynROS8604_RS00100 WP_370586531.1 23182..24954(-) (pilB/pilB1) [Synechococcus sp. ROS8604]
ATGAGCGCCGACTTCAACCGAATGCCCCTGCCGCCTGCTCTGCCGGCAAAGCTGATTTCAGAAGCAGAGCTCGCCGCTGG
AGAAGCACTTCTGCAATCAGGGAAGGTTTTGGACCTGGAAACCTGGAAACAACTTCAAGCGCTCCCCATTCATCTCAGCG
TCGACACCCTCATGGTGGCCATCGCAAGCAGCAGCAACCGAGAGAATCGTGAACAACTCAAGCAGATTTTAAGAAGCCAT
GGCTATATCTCCAAGCTCGTCCTTGCCAATGCAGCGGATCTAAAGCGGATTCTTGATCCACAATCAGTGGACTCAAATTC
GAAGATCAATCAAGCATCAGTTAGCGAAACAGCTAAATCCTTTCTCGATGGATTTGATGTTGAAGGAATTTTGAATGCCG
ATCCAGATGAAGCAGAAATTCAAAGTAATTCAGCCTCATCACTTGACGTCGACCCATCAAGTACTGATCAGTCGCCGATT
ATCACTTTAGTTGATCGTATCTTAGCAAAGGCATTAGACATGAATGCCAGTGATATCCATGTAGAACCACAATATTCAGG
ATTACAAATAAGATTCAGGAAGGATGGAGTCCTGACAAACTTAACGCAACCACTACCCACTAAATTAATTCCAGCCATTA
CCTCTCGTTTCAAGATCATGGCCGATCTTGATATCGCTGAACGGAGGCAAGCACAGGATGGACGTATCCGTAGAAACTAC
AAAGAACGAACCGTTGACTTCCGAGTCAACAGTCTTCCCAGCCGCTACGGGGAAAAGATTTGCCTGCGCTTACTCGATAG
CCAATCCACTCAATTAGGGCTGGATAAACTCATTTCCAATCCCACCACACTCGAGCTTGTGCGAACCCTTGGCTCCAAAC
CATTCGGGATGATTCTTGTGACCGGTCCAACAGGATCTGGTAAATCCACAACTCTCTATTCCTTGTTAGCGGAACGCAAC
GAACCTGGGATCAATATCTCCACCGTAGAAGATCCCATTGAATACACATTGCCTGGTATTACCCAATGTCAGGTGAATCG
TGAGAAAGGCTTTGACTTCAGCACTGCTCTTCGCGCCTTCATGCGCCAGGACCCAGATGTGCTGTTAGTGGGTGAGACCC
GTGATCAAGAAACAGCAAAAACTGCCATCGAAGCAGCTCTGACAGGACATCTGGTCTTAACCACCCTGCACTGCAACGAT
GCCCCGAGCGCCATCGCCCGCCTCGACGAAATGGGCGTTGAACCATTCATGGTGAGCGCATCCTTGCTTGGCATCGTCTC
CCAACGCTTACTCAGGCGCGTCTGCAGCGATTGCCGTATCCCTTATCACCCCGATTCCCAAAAGCTGGGACGCTTTGGCC
TGCTAACGAGCCATGAAGGCGATGTCACCTTTTTCAAAGCCAAACACCACGAAAGACAACAATCGGCATGCCCTCGATGC
CAGGGCACGGGTTACAAGGGCCGTATCGGTGTGTACGAAGTGCTTCGCATGAATGAAGCGCTGGCGGCATCCGTCGCCAA
AGGCGCTACCACTGATCTCGTCCGCCAGTTGGCACTCGAAGCAGGTATGAAAACCTTGCTCGGCTACAGCCTTGATCTCG
TCCGCGAAGGTCACACCACCCTGGAAGAGGTGGACCGGATGGTGTTGACAGATGCAGGGCTGGAATCGGAACAACGAGCC
AGGGCCCTTAGCACTATCACTTGCCGGGGATGTGGTGGCGGACTGCAAGAAGGTTGGCTGGAATGCCTGTATTGCCTGAC
ACCGCGTCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-99)

Type II/IV secretion system protein (T2SSE)

(167-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8FYP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

55.166

96.78

0.534

Multiple sequence alignment