Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynRS9909_RS00095 Genome accession   NZ_CP047943
Coordinates   21611..22687 (-) Length   358 a.a.
NCBI ID   WP_007101117.1    Uniprot ID   A0AAE7J6M0
Organism   Synechococcus sp. RS9909     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16611..27687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynRS9909_RS00070 (SynRS9909_00014) - 16973..17479 (+) 507 WP_007101122.1 RNA-binding protein -
  SynRS9909_RS00075 (SynRS9909_00015) dusA 17524..18528 (-) 1005 WP_007101121.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynRS9909_RS00080 (SynRS9909_00016) msrB 18597..19109 (+) 513 WP_007101120.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynRS9909_RS00085 (SynRS9909_00017) - 19036..20349 (+) 1314 WP_050752467.1 NAD(P)/FAD-dependent oxidoreductase -
  SynRS9909_RS00090 (SynRS9909_00018) - 20342..21598 (-) 1257 WP_007101118.1 type II secretion system F family protein -
  SynRS9909_RS00095 (SynRS9909_00019) pilT 21611..22687 (-) 1077 WP_007101117.1 type IV pilus twitching motility protein PilT Machinery gene
  SynRS9909_RS00100 (SynRS9909_00020) pilB/pilB1 22698..24335 (-) 1638 WP_007101116.1 GspE/PulE family protein Machinery gene
  SynRS9909_RS00105 (SynRS9909_00021) grpE 24444..25178 (+) 735 WP_007101115.1 nucleotide exchange factor GrpE -
  SynRS9909_RS00110 (SynRS9909_00022) dnaJ 25223..26350 (+) 1128 WP_007101114.1 molecular chaperone DnaJ -
  SynRS9909_RS00115 (SynRS9909_00023) - 26353..26595 (+) 243 WP_007101113.1 sulfurtransferase TusA family protein -
  SynRS9909_RS00120 (SynRS9909_00024) rsgA 26582..27484 (+) 903 WP_007101112.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39021.53 Da        Isoelectric Point: 6.1664

>NTDB_id=418614 SynRS9909_RS00095 WP_007101117.1 21611..22687(-) (pilT) [Synechococcus sp. RS9909]
MELMIEDLMEQLVQGSGSDLHLASGQPPYGRFNGQLRPLSESPLSEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGNTIPSIASLELPPVVVDTCARPRGLVLVTGPTGSGKTTTLAALLDHINHSRSEHILTI
EDPIEFVYRSDRSMVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLVAVFAQTLCRRLDPKPGQFGRVMAQEILINTAAIANLIREGKTAQLYSQLQTGADLGM
QTLERALADLVTAGSISRDEAMAKAGKPGELDRLLGGS

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418614 SynRS9909_RS00095 WP_007101117.1 21611..22687(-) (pilT) [Synechococcus sp. RS9909]
ATGGAGCTGATGATCGAAGACCTGATGGAGCAGCTGGTGCAGGGCAGCGGCAGCGACCTGCATCTGGCCAGCGGGCAGCC
GCCCTATGGGCGCTTCAACGGACAGCTCCGCCCCCTCTCGGAATCGCCGCTCAGCGAAGAAAGCTGCAACCGACTGATCT
TTTCGATGCTCAACAACAGCCAGCGCAAGACCCTGGAGCAAACCTGGGAGCTGGATTGCGCCTATGGCCTCAAGGGGGTG
GCCCGCTTCCGCGTCAACGTGTATCGGCAGAAGGGGAGCTACGCCGCCTGCCTGCGGGCCCTGGGCAACACGATCCCCAG
CATCGCCTCGCTGGAGCTGCCGCCGGTGGTGGTGGACACCTGTGCCCGACCGCGGGGCCTGGTGCTGGTGACCGGACCGA
CTGGATCGGGCAAAACCACCACCCTCGCCGCCCTGCTCGACCACATCAACCACAGCCGCAGCGAACACATCCTCACGATC
GAAGACCCGATCGAGTTCGTGTATCGCAGCGACCGCAGCATGGTGCACCAGCGCCAGCTCAATGAAGACACCCGCAGCTT
TGCCAATGCACTGCGCGCCGCCTTGCGCGAAGACCCGGACGTGATCCTGGTGGGTGAAATGCGCGATCTCGAAACGATCC
AGCTGGCGATCACCGCAGCGGAAACCGGTCATCTGGTGTTTGGAACCCTGCACACCAGTTCCGCCGCCCAGACGGTGGAT
CGGATGGTGGATGTGTTCCCTCCCGGCCAGCAAACCCAGATCCGGGTGCAACTCTCCGGCAGCCTGGTGGCCGTTTTCGC
CCAGACCCTCTGCCGTCGGCTCGATCCGAAACCGGGGCAATTCGGCCGGGTGATGGCCCAGGAAATTCTGATCAACACAG
CGGCGATCGCCAACCTGATCCGTGAAGGCAAAACCGCCCAGCTCTATTCCCAGCTGCAAACCGGTGCGGATCTGGGCATG
CAGACCCTCGAGCGGGCCCTGGCCGATCTGGTGACGGCCGGCAGCATCAGCCGGGATGAGGCCATGGCCAAAGCGGGCAA
ACCGGGTGAACTGGACCGCTTGCTGGGCGGATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

51.744

96.089

0.497
  pilT Pseudomonas aeruginosa PAK

51.744

96.089

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.571

97.765

0.494
  pilT Acinetobacter baylyi ADP1

50.872

96.089

0.489
  pilT Neisseria meningitidis 8013

50.729

95.81

0.486
  pilT Legionella pneumophila strain ERS1305867

51.026

95.251

0.486
  pilT Legionella pneumophila strain Lp02

51.026

95.251

0.486
  pilT Acinetobacter baumannii D1279779

50.291

96.089

0.483
  pilT Acinetobacter nosocomialis M2

50.291

96.089

0.483
  pilT Acinetobacter baumannii strain A118

50.291

96.089

0.483
  pilT Neisseria gonorrhoeae MS11

50.437

95.81

0.483
  pilT Vibrio cholerae strain A1552

49.706

94.972

0.472
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.706

94.972

0.472
  pilU Pseudomonas stutzeri DSM 10701

39.193

96.927

0.38
  pilU Vibrio cholerae strain A1552

38.04

96.927

0.369

Multiple sequence alignment