Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynRS9909_RS00100 Genome accession   NZ_CP047943
Coordinates   22698..24335 (-) Length   545 a.a.
NCBI ID   WP_007101116.1    Uniprot ID   A0AAE7J6T3
Organism   Synechococcus sp. RS9909     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17698..29335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynRS9909_RS00080 (SynRS9909_00016) msrB 18597..19109 (+) 513 WP_007101120.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynRS9909_RS00085 (SynRS9909_00017) - 19036..20349 (+) 1314 WP_050752467.1 NAD(P)/FAD-dependent oxidoreductase -
  SynRS9909_RS00090 (SynRS9909_00018) - 20342..21598 (-) 1257 WP_007101118.1 type II secretion system F family protein -
  SynRS9909_RS00095 (SynRS9909_00019) pilT 21611..22687 (-) 1077 WP_007101117.1 type IV pilus twitching motility protein PilT Machinery gene
  SynRS9909_RS00100 (SynRS9909_00020) pilB/pilB1 22698..24335 (-) 1638 WP_007101116.1 GspE/PulE family protein Machinery gene
  SynRS9909_RS00105 (SynRS9909_00021) grpE 24444..25178 (+) 735 WP_007101115.1 nucleotide exchange factor GrpE -
  SynRS9909_RS00110 (SynRS9909_00022) dnaJ 25223..26350 (+) 1128 WP_007101114.1 molecular chaperone DnaJ -
  SynRS9909_RS00115 (SynRS9909_00023) - 26353..26595 (+) 243 WP_007101113.1 sulfurtransferase TusA family protein -
  SynRS9909_RS00120 (SynRS9909_00024) rsgA 26582..27484 (+) 903 WP_007101112.1 ribosome small subunit-dependent GTPase A -
  SynRS9909_RS00125 (SynRS9909_00025) - 27459..27800 (-) 342 WP_007101111.1 YbaB/EbfC family nucleoid-associated protein -
  SynRS9909_RS00130 (SynRS9909_00026) murB 27833..28750 (-) 918 WP_007101110.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 545 a.a.        Molecular weight: 60069.03 Da        Isoelectric Point: 5.1938

>NTDB_id=418615 SynRS9909_RS00100 WP_007101116.1 22698..24335(-) (pilB/pilB1) [Synechococcus sp. RS9909]
MTVHAPAAAAQNPWHQQRLELELLLQVPVPERLDAAADAQLQEAMRRKDLTQQLQPLLARRDDSRDDSRDTPAPSLLDGF
DPEGVLEDDADENARLAGLHEDLEQSLRDSETSPVVNLVNRILLQALQLGASDIHVEPQQAGLQVRFRQDGVLQSYSEPL
PSRLVPAVTSRFKIMADLDIAERRQAQDGRIRRRFQERTVDFRVNSLPSRFGEKIVLRLLDSSATQLGLDTLITDPAALD
LVRNLGAKPFGMILVTGPTGSGKSTTLYSLLAERNDPGINISTVEDPIEYTLPGITQCQVNRDKGFDFSTALRAFMRQDP
DVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLSEMGVEPFMVSASLIGIVSQRLLRKVCHHCCESYQPD
ASELGRFGLMTHNEADVRFVRAHHHDGDGQACPHCQGSGYRGRIGVYEVLRMNEELATAVARGATTDVIRRLALESGMTT
LLGYSLELVRRGQTTLEEVGRMILTDAGLESERRARALHSLTCQGCGGGLQEGWLECPYCLTPRQ

Nucleotide


Download         Length: 1638 bp        

>NTDB_id=418615 SynRS9909_RS00100 WP_007101116.1 22698..24335(-) (pilB/pilB1) [Synechococcus sp. RS9909]
TTGACCGTCCACGCACCTGCCGCCGCTGCACAGAACCCGTGGCACCAGCAGCGACTGGAGCTGGAGCTGCTGCTTCAGGT
TCCTGTGCCGGAGCGGCTCGATGCCGCCGCTGATGCGCAACTGCAGGAGGCGATGCGCCGAAAGGATTTAACCCAGCAGC
TGCAACCGCTCCTGGCCCGGCGCGACGACAGCCGCGACGACAGCCGCGACACCCCAGCGCCATCCCTGCTCGATGGGTTC
GACCCGGAGGGAGTGCTTGAGGACGACGCCGACGAGAACGCTCGACTGGCGGGCCTGCACGAAGATCTGGAGCAGAGCCT
GAGGGATTCCGAAACCTCACCGGTGGTGAATCTGGTGAACCGGATCCTGCTGCAGGCCCTGCAACTGGGTGCCAGCGACA
TTCATGTGGAACCGCAGCAGGCAGGCCTGCAGGTGCGGTTCCGACAGGACGGCGTGCTGCAGAGCTACAGCGAACCGTTG
CCGAGTCGGTTAGTGCCCGCCGTGACCTCCCGCTTCAAGATCATGGCCGACCTGGACATCGCCGAACGCCGCCAGGCACA
GGACGGCCGCATCCGCAGGCGCTTCCAGGAGCGCACGGTGGATTTCCGGGTCAACAGCCTGCCGAGCCGCTTCGGCGAAA
AGATTGTGCTGCGCCTGCTCGACAGCTCCGCCACCCAGCTGGGGCTGGACACCTTGATCACCGACCCGGCGGCCCTGGAT
CTGGTGCGCAATCTCGGCGCCAAACCCTTCGGGATGATCCTGGTGACCGGACCGACGGGCTCGGGAAAATCGACCACGCT
GTATTCCCTGCTGGCGGAGCGCAACGACCCGGGAATCAACATCTCCACCGTGGAAGATCCGATCGAATACACCCTGCCGG
GCATCACGCAGTGCCAGGTGAACCGCGACAAAGGCTTTGATTTCAGCACCGCCCTGCGCGCCTTCATGCGTCAGGACCCG
GATGTGCTGCTGGTGGGCGAAACCCGCGACCTGGAGACCGCCAAGACAGCGATCGAAGCGGCGCTCACCGGTCACCTGGT
GCTGACCACACTCCATTGCAACGATGCACCGAGTGCCATCGCCCGCTTGAGCGAGATGGGGGTGGAACCGTTCATGGTCA
GCGCCTCCCTAATCGGCATCGTGTCGCAACGCCTGCTGCGCAAGGTATGCCACCACTGCTGCGAGAGCTATCAGCCCGAT
GCCAGCGAACTGGGGCGATTCGGACTGATGACCCACAACGAAGCGGATGTGCGCTTTGTGCGAGCCCATCACCACGATGG
CGACGGCCAGGCCTGTCCCCACTGCCAGGGCAGTGGATATCGGGGCCGGATAGGGGTCTACGAAGTGCTGCGGATGAATG
AGGAGCTGGCGACGGCGGTGGCCCGTGGCGCCACCACCGACGTGATCCGGCGACTCGCCCTGGAAAGCGGCATGACGACG
CTGCTGGGCTACAGCCTGGAGCTGGTGCGCCGCGGCCAGACGACCCTGGAGGAAGTGGGCCGGATGATCCTCACCGACGC
CGGTCTGGAATCGGAACGCCGCGCCCGAGCCCTGCACAGCCTCACCTGCCAGGGCTGCGGTGGCGGACTGCAGGAAGGCT
GGCTGGAATGTCCGTACTGTCTGACCCCACGTCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-388)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

62.857

83.486

0.525
  pilF Thermus thermophilus HB27

42.348

87.523

0.371

Multiple sequence alignment