Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynBIOSU31_RS00095 Genome accession   NZ_CP047936
Coordinates   22105..23178 (-) Length   357 a.a.
NCBI ID   WP_186491199.1    Uniprot ID   A0A7G8DIQ5
Organism   Synechococcus sp. BIOS-U3-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17105..28178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynBIOSU31_RS00070 (SynBIOSU31_00015) - 17401..17970 (+) 570 WP_186491193.1 RNA-binding protein -
  SynBIOSU31_RS00075 (SynBIOSU31_00016) dusA 17995..18999 (-) 1005 WP_186491194.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynBIOSU31_RS00080 (SynBIOSU31_00017) msrB 19111..19578 (+) 468 WP_186491195.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynBIOSU31_RS00085 (SynBIOSU31_00018) - 19508..20836 (+) 1329 WP_186491196.1 NAD(P)/FAD-dependent oxidoreductase -
  SynBIOSU31_RS00090 (SynBIOSU31_00019) - 20811..22088 (-) 1278 WP_186491197.1 type II secretion system F family protein -
  SynBIOSU31_RS00095 (SynBIOSU31_00020) pilT 22105..23178 (-) 1074 WP_186491199.1 type IV pilus twitching motility protein PilT Machinery gene
  SynBIOSU31_RS00100 (SynBIOSU31_00021) pilB/pilB1 23189..24847 (-) 1659 WP_255477284.1 GspE/PulE family protein Machinery gene
  SynBIOSU31_RS00105 (SynBIOSU31_00022) grpE 24943..25680 (+) 738 WP_186492726.1 nucleotide exchange factor GrpE -
  SynBIOSU31_RS00110 (SynBIOSU31_00023) dnaJ 25723..26853 (+) 1131 WP_186491202.1 molecular chaperone DnaJ -
  SynBIOSU31_RS00115 (SynBIOSU31_00024) - 26856..27098 (+) 243 WP_186491207.1 sulfurtransferase TusA family protein -
  SynBIOSU31_RS00120 (SynBIOSU31_00025) rsgA 27118..27999 (+) 882 WP_186491212.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39086.62 Da        Isoelectric Point: 6.3057

>NTDB_id=418539 SynBIOSU31_RS00095 WP_186491199.1 22105..23178(-) (pilT) [Synechococcus sp. BIOS-U3-1]
MELMIEDLMQELVDNGGSDLHLASGQPPYGRFSGKLRPMRDEALLEDSCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIELLNLPAVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDQSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLLAVFSQTLCQRNNPSPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGALGM
QTLEKALANLVTHGDINLQEALAKASKPSELERLVSS

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=418539 SynBIOSU31_RS00095 WP_186491199.1 22105..23178(-) (pilT) [Synechococcus sp. BIOS-U3-1]
ATGGAGCTGATGATCGAGGACCTGATGCAGGAGCTGGTGGACAACGGTGGCAGTGACCTGCATCTCGCCAGCGGCCAACC
CCCCTACGGACGTTTCAGCGGCAAACTACGGCCGATGCGTGATGAAGCCCTGCTGGAGGACAGCTGCAACCGGCTGATCT
TCTCGATGCTCAACAACAGCCAACGCAAGACCCTTGAGCAGACGTGGGAACTCGACTGCGCTTACGGCCTCAAAGGAGTG
GCCCGTTTCCGGGTGAACGTTTATCGCCAGAAGGGGAGCTATGCGGCCTGCTTAAGGGCACTGGGCAGCACGATCCCAAG
CATCGAATTGCTGAACCTGCCTGCAGTGGTGGTGGAAACCAGCAAACGACCTCGGGGCCTAGTGCTGGTGACTGGACCCA
CCGGCTCGGGCAAGACCACCACCCTGGCCGCGTTGCTGGATCACATCAATCACAGCCGAGCCGAGCACATCCTCACGATC
GAGGACCCGATCGAATTCGTCTACAAAAGCGACCAAAGCCTGGTTCACCAGCGTCAGCTCAACGAGGACACCCGCAGCTT
TGGGAATGCACTCAGAGCAGCACTGCGGGAAGACCCAGACGTGATCCTGGTTGGGGAGATGCGGGACTTGGAAACCATTC
AGCTGGCCATCAGCGCCGCGGAGACAGGTCACCTCGTCTTCGGCACTCTGCACACGAGTTCCGCAGCCCAAACGGTTGAT
CGGATGGTTGATGTGTTCCCGCCTGGCCAGCAGACCCAGATCCGTGTGCAACTGTCAGGGAGCTTGTTGGCAGTGTTTTC
ACAGACGCTTTGCCAACGCAACAACCCGTCTCCAGGTCAGTTCGGCCGAGTGATGGCTCAGGAAATCATGATCAACACCC
CTGCCATCGCCAACCTGATCCGCGAGGGCAAGACCGCTCAGCTGTATTCACAGATTCAGACCGGAGGCGCACTGGGGATG
CAGACCCTGGAAAAGGCTCTGGCCAATCTGGTGACTCACGGCGATATCAACCTGCAGGAAGCACTGGCCAAGGCCAGCAA
GCCCTCAGAGCTGGAACGACTTGTGAGCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8DIQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.026

97.199

0.515
  pilT Pseudomonas aeruginosa PAK

53.026

97.199

0.515
  pilT Acinetobacter baumannii strain A118

51.873

97.199

0.504
  pilT Acinetobacter nosocomialis M2

51.873

97.199

0.504
  pilT Acinetobacter baumannii D1279779

51.873

97.199

0.504
  pilT Acinetobacter baylyi ADP1

51.585

97.199

0.501
  pilT Legionella pneumophila strain ERS1305867

52.522

94.398

0.496
  pilT Legionella pneumophila strain Lp02

52.522

94.398

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.148

98.599

0.485
  pilT Neisseria gonorrhoeae MS11

50.437

96.078

0.485
  pilT Neisseria meningitidis 8013

51.497

93.557

0.482
  pilT Vibrio cholerae strain A1552

50.588

95.238

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.588

95.238

0.482
  pilU Pseudomonas stutzeri DSM 10701

38.177

98.319

0.375
  pilU Vibrio cholerae strain A1552

37.283

96.919

0.361

Multiple sequence alignment