Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynBIOSU31_RS00100 Genome accession   NZ_CP047936
Coordinates   23189..24847 (-) Length   552 a.a.
NCBI ID   WP_255477284.1    Uniprot ID   -
Organism   Synechococcus sp. BIOS-U3-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18189..29847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynBIOSU31_RS00080 (SynBIOSU31_00017) msrB 19111..19578 (+) 468 WP_186491195.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynBIOSU31_RS00085 (SynBIOSU31_00018) - 19508..20836 (+) 1329 WP_186491196.1 NAD(P)/FAD-dependent oxidoreductase -
  SynBIOSU31_RS00090 (SynBIOSU31_00019) - 20811..22088 (-) 1278 WP_186491197.1 type II secretion system F family protein -
  SynBIOSU31_RS00095 (SynBIOSU31_00020) pilT 22105..23178 (-) 1074 WP_186491199.1 type IV pilus twitching motility protein PilT Machinery gene
  SynBIOSU31_RS00100 (SynBIOSU31_00021) pilB/pilB1 23189..24847 (-) 1659 WP_255477284.1 GspE/PulE family protein Machinery gene
  SynBIOSU31_RS00105 (SynBIOSU31_00022) grpE 24943..25680 (+) 738 WP_186492726.1 nucleotide exchange factor GrpE -
  SynBIOSU31_RS00110 (SynBIOSU31_00023) dnaJ 25723..26853 (+) 1131 WP_186491202.1 molecular chaperone DnaJ -
  SynBIOSU31_RS00115 (SynBIOSU31_00024) - 26856..27098 (+) 243 WP_186491207.1 sulfurtransferase TusA family protein -
  SynBIOSU31_RS00120 (SynBIOSU31_00025) rsgA 27118..27999 (+) 882 WP_186491212.1 ribosome small subunit-dependent GTPase A -
  SynBIOSU31_RS00125 (SynBIOSU31_00026) - 27974..28315 (-) 342 WP_066908605.1 YbaB/EbfC family nucleoid-associated protein -
  SynBIOSU31_RS00130 (SynBIOSU31_00027) murB 28340..29272 (-) 933 WP_186491214.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 552 a.a.        Molecular weight: 60425.74 Da        Isoelectric Point: 5.1255

>NTDB_id=418540 SynBIOSU31_RS00100 WP_255477284.1 23189..24847(-) (pilB/pilB1) [Synechococcus sp. BIOS-U3-1]
MSDDRRHLELELLLQRTIPGKLVSADEPSLLQELEAMGFEPARHAALAERLNALLNRPSEDASPTPQTTTTTTDILGEKA
TSYLQDFSVEAVLVQDPDEASAQSAGPEDLESSLSDADQSPVINLVDRILMQALELGASDIHVEPQQAGLQLRFRQDGVL
QSHIEPLPSRLIPAVTSRFKIMAELDIAERRVAQDGRIRRRFQNRTVDFRVNSLPSRFGEKIVLRLLDSSATQLGLDKLI
SNPRTLELVRALGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFDFSQALR
AFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLDEMGVEPFMVSASLIGIVSQRLLRRVCHHC
QIAYRPDPEELGRFGLMASNEANVSFVKANHHEGQVNPCPHCQGSGYKGRVGVYEVLRMNEALAAAVAKGATTDMVRQLA
LEGGMKTLLGYSLELVREGHTTLEEVGRMVLTDAGLESERRARALSTLTCHGCGGGLQEGWLECPYCLTPRQ

Nucleotide


Download         Length: 1659 bp        

>NTDB_id=418540 SynBIOSU31_RS00100 WP_255477284.1 23189..24847(-) (pilB/pilB1) [Synechococcus sp. BIOS-U3-1]
GTGAGCGACGACCGCCGTCATCTGGAACTGGAACTTCTGTTGCAGCGGACCATCCCAGGCAAGCTCGTCAGCGCCGATGA
ACCCTCATTGCTCCAGGAGCTGGAAGCGATGGGTTTTGAACCTGCACGTCACGCTGCCCTTGCCGAGCGCCTTAACGCAC
TGCTGAACAGGCCATCCGAGGACGCCTCACCCACACCTCAGACCACCACTACAACGACTGACATCCTGGGAGAGAAAGCG
ACCTCCTACCTACAGGACTTTTCGGTGGAAGCGGTTCTGGTTCAGGATCCCGACGAGGCATCAGCACAAAGCGCAGGTCC
TGAAGATCTCGAATCCAGCCTCAGCGATGCAGACCAGTCGCCAGTGATCAATCTGGTGGACCGGATCCTAATGCAGGCTC
TAGAGCTTGGGGCCAGCGACATCCATGTGGAACCACAGCAGGCCGGACTTCAGCTGCGCTTCCGTCAAGACGGTGTGCTT
CAGAGCCACATCGAACCCCTGCCCAGCCGACTGATTCCCGCCGTGACTTCACGGTTCAAAATCATGGCGGAGCTGGACAT
TGCCGAACGCCGCGTTGCTCAGGATGGACGTATCCGGCGCCGTTTCCAGAACCGCACGGTGGATTTCCGTGTCAACAGTC
TGCCGAGCCGCTTCGGCGAAAAGATCGTGCTGCGGCTACTCGACAGCTCTGCCACCCAGCTGGGACTCGACAAGCTGATT
TCCAATCCTCGAACGCTGGAGCTGGTCCGCGCCCTCGGCTCAAAACCCTTCGGAATGATCCTGGTCACAGGGCCGACTGG
ATCCGGTAAATCCACAACGCTGTACTCCCTGCTGGCGGAACGCAATGAACCAGGCATCAACATTTCCACCGTGGAGGACC
CCATCGAATACACCTTGCCCGGCATTACCCAATGCCAGGTGAACCGGGAGAAGGGATTTGATTTCAGTCAGGCACTGAGA
GCCTTCATGCGCCAGGACCCGGATGTACTGCTGGTGGGGGAAACGCGCGATCTGGAAACAGCGAAAACGGCCATCGAAGC
TGCGCTCACAGGCCACCTGGTGTTGACGACCCTGCACTGCAATGACGCCCCCAGTGCGATTGCCCGACTGGATGAGATGG
GCGTGGAACCTTTCATGGTCAGTGCCTCATTGATCGGGATCGTCTCGCAACGTCTTCTGCGAAGGGTCTGTCACCACTGC
CAGATCGCCTACCGCCCCGATCCCGAAGAGTTGGGTCGCTTCGGCCTGATGGCGAGCAACGAAGCCAACGTGAGCTTTGT
GAAAGCCAACCATCACGAAGGCCAGGTCAATCCTTGTCCCCACTGTCAGGGCAGCGGTTACAAGGGCCGCGTGGGGGTCT
ATGAAGTGCTGCGCATGAATGAGGCTCTGGCGGCCGCCGTGGCCAAAGGCGCCACGACGGACATGGTGCGCCAGCTGGCT
CTGGAGGGAGGGATGAAGACCCTGCTGGGCTACAGCCTGGAGCTAGTACGGGAAGGACATACAACCCTGGAGGAAGTGGG
CCGAATGGTTCTCACAGACGCCGGACTTGAATCGGAACGCCGTGCCCGCGCCCTCAGCACCCTGACCTGTCATGGATGTG
GTGGCGGACTCCAGGAAGGTTGGCTGGAATGTCCCTACTGCCTGACACCGCGCCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.639

82.246

0.54

Multiple sequence alignment