Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THETH_RS08400 Genome accession   NC_015707
Coordinates   1688696..1689754 (+) Length   352 a.a.
NCBI ID   WP_013932925.1    Uniprot ID   F7YVQ2
Organism   Pseudothermotoga thermarum DSM 5069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1683696..1694754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THETH_RS08390 (Theth_1668) - 1685579..1685941 (+) 363 WP_013932923.1 response regulator -
  THETH_RS08395 (Theth_1669) ileS 1685956..1688691 (+) 2736 WP_041446444.1 isoleucine--tRNA ligase -
  THETH_RS08400 (Theth_1670) pilT 1688696..1689754 (+) 1059 WP_013932925.1 type IV pilus twitching motility protein PilT Machinery gene
  THETH_RS08405 (Theth_1671) prfA 1689751..1690809 (+) 1059 WP_013932926.1 peptide chain release factor 1 -
  THETH_RS10740 (Theth_1672) - 1690806..1691075 (-) 270 WP_013932927.1 rod-binding protein -
  THETH_RS08415 (Theth_1673) - 1691077..1692072 (-) 996 WP_013932928.1 flagellar basal body P-ring protein FlgI -
  THETH_RS08420 (Theth_1674) - 1692083..1692679 (-) 597 WP_013932929.1 flagellar basal body L-ring protein FlgH -
  THETH_RS08425 (Theth_1675) flgA 1692684..1693355 (-) 672 WP_041446445.1 flagellar basal body P-ring formation chaperone FlgA -
  THETH_RS08430 (Theth_1676) flgG 1693377..1694162 (-) 786 WP_013932931.1 flagellar basal-body rod protein FlgG -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39826.95 Da        Isoelectric Point: 6.8396

>NTDB_id=41565 THETH_RS08400 WP_013932925.1 1688696..1689754(+) (pilT) [Pseudothermotoga thermarum DSM 5069]
MLDIFALVSDAFNKKASDVHLSVGCHPVYRINGLLELQRNYPVITEKDLLDSIERLFERIDFHALENKKEIDFAFDVGEE
IRVRGNLYYERRKPALALRLITKRIRTFEELGLPSILKEFAERDSGLILVAGPTGSGKSTTLAAIIDHINSNYPYHIITI
EDPIEYVFTNKKSVVHQRELGQDTKSFYDGLKYALRQDPDVILVGEMRDLETMALALTAAETGHLVLSTIHTNSAATAPE
RIIDVFPPHQQKQVALQLANTLVAVIYQRLLRRADGKGMVPILEILVGTPAVKNLIRENKLHQIESIMQTSAKQGMVLFD
DALFKAYVNGLIDKSQVYEYARNPEEMRRKIG

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=41565 THETH_RS08400 WP_013932925.1 1688696..1689754(+) (pilT) [Pseudothermotoga thermarum DSM 5069]
ATGTTGGACATATTTGCTTTGGTATCCGATGCCTTCAACAAGAAGGCATCGGATGTTCATTTGTCCGTCGGTTGTCATCC
AGTTTACCGTATAAATGGATTACTTGAACTTCAAAGGAATTATCCTGTTATAACTGAAAAAGACCTTTTGGACAGCATCG
AAAGACTTTTTGAACGCATAGATTTTCACGCCCTTGAGAACAAAAAAGAAATAGACTTTGCGTTTGATGTTGGAGAAGAA
ATAAGGGTACGTGGCAACCTTTATTACGAACGCAGAAAGCCTGCATTGGCACTTCGTTTGATAACCAAAAGAATACGGAC
CTTCGAAGAACTTGGTTTGCCTTCCATTTTAAAAGAATTCGCCGAGCGCGATTCGGGGCTCATACTCGTTGCAGGGCCAA
CTGGGAGTGGTAAATCCACAACTTTGGCTGCTATTATCGACCATATAAACTCCAATTACCCGTATCACATAATCACTATA
GAAGATCCCATAGAGTACGTTTTTACGAACAAAAAGTCTGTAGTTCACCAAAGAGAACTAGGTCAGGACACAAAGAGTTT
TTACGATGGACTAAAGTATGCCCTCAGACAGGATCCGGATGTGATACTCGTTGGAGAAATGCGAGACCTTGAAACGATGG
CTTTGGCTCTCACAGCTGCAGAAACAGGTCATTTGGTGCTCAGTACGATACACACAAACAGTGCCGCAACTGCACCGGAA
AGAATTATAGATGTTTTTCCACCTCATCAACAAAAGCAAGTGGCGCTTCAGCTTGCCAATACTTTGGTGGCGGTTATATA
CCAAAGGCTTTTAAGAAGAGCTGATGGAAAAGGTATGGTGCCAATCCTTGAGATTTTGGTTGGTACCCCAGCGGTAAAGA
ACTTGATACGCGAAAACAAACTGCATCAGATAGAATCTATAATGCAAACCAGTGCCAAGCAGGGTATGGTGTTGTTTGAC
GATGCTCTGTTCAAAGCCTACGTAAATGGTTTGATAGACAAATCTCAGGTTTACGAGTACGCTAGAAACCCTGAGGAGAT
GAGGAGAAAGATAGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F7YVQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.433

99.716

0.483
  pilT Neisseria gonorrhoeae MS11

46.11

98.58

0.455
  pilT Neisseria meningitidis 8013

46.11

98.58

0.455
  pilT Acinetobacter baumannii D1279779

45.977

98.864

0.455
  pilT Acinetobacter baumannii strain A118

45.977

98.864

0.455
  pilT Acinetobacter nosocomialis M2

45.977

98.864

0.455
  pilT Legionella pneumophila strain ERS1305867

45.906

97.159

0.446
  pilT Legionella pneumophila strain Lp02

45.906

97.159

0.446
  pilT Pseudomonas aeruginosa PAK

44.828

98.864

0.443
  pilT Pseudomonas stutzeri DSM 10701

44.828

98.864

0.443
  pilT Acinetobacter baylyi ADP1

44.928

98.011

0.44
  pilT Vibrio cholerae strain A1552

45.104

95.739

0.432
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.104

95.739

0.432
  pilU Acinetobacter baylyi ADP1

37.989

100

0.386
  pilU Pseudomonas stutzeri DSM 10701

39.763

95.739

0.381
  pilU Vibrio cholerae strain A1552

39.82

94.886

0.378

Multiple sequence alignment