Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GV243_RS21355 Genome accession   NZ_CP047679
Coordinates   4470571..4471593 (-) Length   340 a.a.
NCBI ID   WP_047573684.1    Uniprot ID   -
Organism   Serratia marcescens strain 4201     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4465571..4476593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GV243_RS21325 (GV243_21325) - 4466372..4466884 (+) 513 WP_033644682.1 SprT family zinc-dependent metalloprotease -
  GV243_RS21330 (GV243_21330) endA 4466986..4467681 (+) 696 WP_033644681.1 deoxyribonuclease I -
  GV243_RS21335 (GV243_21335) rsmE 4467751..4468482 (+) 732 WP_033644679.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GV243_RS21340 (GV243_21340) gshB 4468493..4469443 (+) 951 WP_033644677.1 glutathione synthase -
  GV243_RS21345 (GV243_21345) - 4469589..4470152 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  GV243_RS21350 (GV243_21350) ruvX 4470152..4470574 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  GV243_RS21355 (GV243_21355) pilT 4470571..4471593 (-) 1023 WP_047573684.1 type IV pilus twitching motility protein PilT Machinery gene
  GV243_RS21360 (GV243_21360) - 4471614..4472321 (+) 708 WP_160204568.1 YggS family pyridoxal phosphate-dependent enzyme -
  GV243_RS21365 (GV243_21365) proC 4472341..4473162 (+) 822 WP_047573680.1 pyrroline-5-carboxylate reductase -
  GV243_RS21370 (GV243_21370) - 4473194..4473748 (+) 555 WP_033644671.1 YggT family protein -
  GV243_RS21375 (GV243_21375) yggU 4473745..4474035 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  GV243_RS21380 (GV243_21380) - 4474053..4474646 (+) 594 WP_042785371.1 XTP/dITP diphosphatase -
  GV243_RS21385 (GV243_21385) hemW 4474639..4475781 (+) 1143 WP_160204569.1 radical SAM family heme chaperone HemW -
  GV243_RS21390 (GV243_21390) - 4475820..4476254 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36310.55 Da        Isoelectric Point: 7.0599

>NTDB_id=415261 GV243_RS21355 WP_047573684.1 4470571..4471593(-) (pilT) [Serratia marcescens strain 4201]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHCSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=415261 GV243_RS21355 WP_047573684.1 4470571..4471593(-) (pilT) [Serratia marcescens strain 4201]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCATTGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTACGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment