Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GT391_RS11390 Genome accession   NZ_CP047495
Coordinates   2540455..2541471 (+) Length   338 a.a.
NCBI ID   WP_010279096.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain 1692     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2535455..2546471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GT391_RS11355 (GT391_11355) - 2535680..2535940 (-) 261 WP_072014188.1 type II toxin-antitoxin system ParD family antitoxin -
  GT391_RS11360 (GT391_11360) hemW 2536169..2537311 (-) 1143 WP_010279117.1 radical SAM family heme chaperone HemW -
  GT391_RS11365 (GT391_11365) rdgB 2537304..2537897 (-) 594 WP_010279113.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  GT391_RS11370 (GT391_11370) yggU 2537915..2538205 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  GT391_RS11375 (GT391_11375) - 2538202..2538756 (-) 555 WP_010279107.1 YggT family protein -
  GT391_RS11380 (GT391_11380) proC 2538805..2539626 (-) 822 WP_039317070.1 pyrroline-5-carboxylate reductase -
  GT391_RS11385 (GT391_11385) - 2539721..2540434 (-) 714 WP_010279099.1 YggS family pyridoxal phosphate-dependent enzyme -
  GT391_RS11390 (GT391_11390) pilT 2540455..2541471 (+) 1017 WP_010279096.1 type IV pilus twitching motility protein PilT Machinery gene
  GT391_RS11395 (GT391_11395) - 2541440..2543272 (-) 1833 WP_010279093.1 sensor domain-containing diguanylate cyclase -
  GT391_RS11400 (GT391_11400) - 2543802..2545373 (-) 1572 WP_010279090.1 methyl-accepting chemotaxis protein -
  GT391_RS11405 (GT391_11405) nsrR 2545682..2546107 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36628.83 Da        Isoelectric Point: 6.4934

>NTDB_id=413865 GT391_RS11390 WP_010279096.1 2540455..2541471(+) (pilT) [Pectobacterium brasiliense strain 1692]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLTTLQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRCCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=413865 GT391_RS11390 WP_010279096.1 2540455..2541471(+) (pilT) [Pectobacterium brasiliense strain 1692]
ATGGAATTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCAGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCCGCTCAGCGCGAGCAACTACGGCAGGCTGGGCAGGTGGACGGCGCGCTAATGCTGCCCGATGGGCAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTATTATTCCGTCCGCTCAGCCTTCATT
GACGACGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGAGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGATGTTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCATCGTTTGC
GCAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGGGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTATCCACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCAGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACTTGTTTGCAGGCGGTAGTGACGCA
AAAACTGCTGCCCGCTGCGCAAGGTGGCCGAATCGCGCTTTATGAAGTGCTGACGGCGACTGCGGCGGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAACGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367

Multiple sequence alignment