Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FLEXSI_RS06955 Genome accession   NC_015672
Coordinates   1466671..1467729 (-) Length   352 a.a.
NCBI ID   WP_013886504.1    Uniprot ID   F8E7V3
Organism   Flexistipes sinusarabici DSM 4947     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1461671..1472729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLEXSI_RS06940 (Flexsi_1368) tsaD 1462704..1463696 (+) 993 WP_013886501.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  FLEXSI_RS06945 (Flexsi_1369) - 1463668..1464645 (+) 978 WP_013886502.1 tRNA-dihydrouridine synthase family protein -
  FLEXSI_RS06950 (Flexsi_1370) - 1465065..1466666 (-) 1602 WP_013886503.1 NFACT RNA binding domain-containing protein -
  FLEXSI_RS06955 (Flexsi_1371) pilT 1466671..1467729 (-) 1059 WP_013886504.1 type IV pilus twitching motility protein PilT Machinery gene
  FLEXSI_RS06960 (Flexsi_1372) - 1467756..1468823 (-) 1068 WP_169310286.1 hypothetical protein -
  FLEXSI_RS06965 (Flexsi_1373) - 1468813..1469853 (-) 1041 WP_013886506.1 hypothetical protein -
  FLEXSI_RS06970 (Flexsi_1374) - 1470291..1470788 (+) 498 WP_013886507.1 PaaI family thioesterase -
  FLEXSI_RS06975 (Flexsi_1375) extS 1470790..1471584 (-) 795 WP_013886508.1 selenite/tellurite reduction operon c-type cytochrome lipoprotein ExtS -
  FLEXSI_RS06980 (Flexsi_1376) extQ 1471566..1471979 (-) 414 WP_013886509.1 selenite/tellurite reduction operon b-type cytochrome membrane protein ExtQ -
  FLEXSI_RS06985 (Flexsi_1377) - 1472210..1472437 (+) 228 WP_013886510.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39324.51 Da        Isoelectric Point: 7.5096

>NTDB_id=41321 FLEXSI_RS06955 WP_013886504.1 1466671..1467729(-) (pilT) [Flexistipes sinusarabici DSM 4947]
MAKIDAFFKYMLENDISDLHLSSGCKPMVRKHGELEEIKYQQLSDEILRPLLYEIISEEQKKKFEKTKDLDFAYEVPGKA
RFRANYFMQKRGACAVFRLIPSKILTADDLGLPNQVLKFAKLSRGLVLVTGPTGSGKSTTLAAIIDYINKSRKEHILTIE
DPVEFVHHSQGCLINHREVETHTESFSAALRAALREDPDVILVGELRDLETIELAITAAETGHLVFGTLHTNSAAKTVDR
IIDAFPSGQQAQIRAMLSESLKGVIAQQLLKKADGKGRVAALELLIVNSAIANLIREGKTFQIPSMIQTGKGDGMQLMDQ
AIMEFLMNKTVTPQEAYVKANDKKSFERFMDK

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=41321 FLEXSI_RS06955 WP_013886504.1 1466671..1467729(-) (pilT) [Flexistipes sinusarabici DSM 4947]
ATGGCTAAAATAGATGCTTTTTTCAAATATATGCTGGAAAATGATATAAGTGACCTCCATTTGAGCTCCGGATGCAAACC
GATGGTCAGAAAACATGGAGAACTTGAAGAGATTAAGTACCAGCAGCTGAGTGATGAAATTTTAAGACCGCTGCTATATG
AAATAATTTCAGAGGAGCAGAAAAAGAAATTTGAGAAAACAAAAGATCTGGATTTTGCCTATGAAGTGCCCGGAAAAGCG
CGCTTCAGGGCAAATTATTTTATGCAGAAAAGAGGAGCATGCGCTGTTTTCAGACTTATTCCCAGTAAAATTCTCACCGC
TGATGACCTGGGGTTACCCAACCAGGTGTTAAAATTTGCAAAACTTTCAAGGGGGCTTGTACTGGTTACCGGTCCTACAG
GCAGCGGGAAATCAACAACGCTGGCTGCGATTATTGATTATATAAACAAATCCAGAAAAGAGCATATTCTAACAATAGAA
GATCCGGTGGAATTTGTACACCATAGCCAGGGATGTTTGATCAACCACAGAGAAGTGGAAACGCATACCGAATCGTTCTC
TGCCGCATTGAGAGCTGCTCTCAGGGAGGACCCGGATGTTATTCTTGTGGGAGAGCTCAGAGACCTTGAAACGATAGAAC
TTGCAATTACAGCCGCTGAAACCGGACATCTGGTTTTCGGTACATTACATACAAATTCGGCTGCAAAAACTGTTGACAGA
ATCATCGACGCATTTCCATCAGGACAACAGGCACAGATAAGGGCAATGCTGTCTGAATCTCTAAAAGGTGTCATAGCGCA
GCAGCTTCTAAAGAAAGCTGACGGTAAGGGGCGTGTGGCTGCTCTTGAGCTGTTAATTGTCAACAGTGCCATTGCCAATC
TTATCAGAGAAGGAAAAACATTTCAGATTCCTTCCATGATTCAAACGGGCAAAGGAGACGGGATGCAACTGATGGACCAG
GCCATTATGGAGTTTCTCATGAATAAAACAGTAACTCCTCAGGAAGCTTATGTGAAAGCTAATGATAAAAAATCTTTTGA
ACGTTTTATGGACAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F8E7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

56.851

97.443

0.554
  pilT Legionella pneumophila strain Lp02

56.851

97.443

0.554
  pilT Acinetobacter baumannii strain A118

55.685

97.443

0.543
  pilT Acinetobacter nosocomialis M2

55.685

97.443

0.543
  pilT Acinetobacter baumannii D1279779

55.685

97.443

0.543
  pilT Pseudomonas stutzeri DSM 10701

56.97

93.75

0.534
  pilT Acinetobacter baylyi ADP1

54.81

97.443

0.534
  pilT Pseudomonas aeruginosa PAK

56.061

93.75

0.526
  pilT Neisseria meningitidis 8013

53.779

97.727

0.526
  pilT Neisseria gonorrhoeae MS11

53.488

97.727

0.523
  pilT Vibrio cholerae strain A1552

53.198

97.727

0.52
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.198

97.727

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.343

95.17

0.489
  pilU Pseudomonas stutzeri DSM 10701

39.429

99.432

0.392
  pilU Vibrio cholerae strain A1552

40.413

96.307

0.389
  pilU Acinetobacter baylyi ADP1

38.095

95.455

0.364

Multiple sequence alignment