Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FLEXSI_RS06190 Genome accession   NC_015672
Coordinates   1294320..1295417 (-) Length   365 a.a.
NCBI ID   WP_013886362.1    Uniprot ID   F8E713
Organism   Flexistipes sinusarabici DSM 4947     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1289320..1300417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLEXSI_RS06180 (Flexsi_1219) - 1291313..1292962 (-) 1650 WP_041262301.1 ATP-binding protein -
  FLEXSI_RS06185 (Flexsi_1220) - 1293029..1294240 (-) 1212 WP_013886361.1 type II secretion system F family protein -
  FLEXSI_RS06190 (Flexsi_1221) pilT 1294320..1295417 (-) 1098 WP_013886362.1 type IV pilus twitching motility protein PilT Machinery gene
  FLEXSI_RS06195 (Flexsi_1222) pilB 1295510..1297207 (-) 1698 WP_013886363.1 type IV-A pilus assembly ATPase PilB -
  FLEXSI_RS06200 (Flexsi_1223) aroE 1297209..1298048 (-) 840 WP_013886364.1 shikimate dehydrogenase -
  FLEXSI_RS06205 (Flexsi_1224) - 1298075..1298524 (-) 450 WP_244403717.1 DUF502 domain-containing protein -
  FLEXSI_RS06210 (Flexsi_1225) - 1298859..1299518 (-) 660 WP_013886366.1 GPR1/FUN34/YaaH family transporter -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 41101.39 Da        Isoelectric Point: 7.5064

>NTDB_id=41320 FLEXSI_RS06190 WP_013886362.1 1294320..1295417(-) (pilT) [Flexistipes sinusarabici DSM 4947]
MYSLQELLQKMIDMGATDLHLTVGSSPVYRVNGMLVRTEGEALSVNDTKKMCYSIMTEAQKKTFEEEMELDLSFGIKGLS
RFRANFFMQRGAMAAAMRRIPYQVMSFEQLGVPKIVESLCEKSRGLVLVTGPTGSGKSTTLASMIDKINHEKKVHIITVE
DPIEYLHNHDKALINQREVNSDTKSFAKALKYILRQDPDVALIGEMRDLETIEAALTVSETGHLTFGTLHTNSAVQTINR
IIDVFPPHQQPQIRAQLSFVLEGVICQQLLQTSDGNSRVLACEVLVPNSGIRNLIREDKLHQIYSMMQSGQAEHGMITMT
QSLYALYRKNLISYEEAVNRAVYPDEVRQLIERGNVVNKKKSGRF

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=41320 FLEXSI_RS06190 WP_013886362.1 1294320..1295417(-) (pilT) [Flexistipes sinusarabici DSM 4947]
ATGTATTCTTTGCAGGAATTATTGCAAAAGATGATAGATATGGGCGCCACAGACCTTCATTTGACTGTGGGTTCCAGCCC
TGTTTACAGAGTAAATGGAATGCTTGTAAGAACAGAAGGTGAAGCACTCAGTGTAAATGATACCAAAAAGATGTGTTACA
GCATAATGACAGAGGCTCAGAAAAAGACTTTTGAAGAGGAGATGGAGCTGGACCTGTCTTTCGGTATAAAAGGGCTCAGC
AGGTTCAGGGCAAACTTTTTTATGCAGCGGGGCGCTATGGCTGCAGCGATGCGCCGAATACCATACCAGGTCATGTCATT
TGAACAGCTCGGCGTACCTAAAATTGTGGAAAGCTTGTGTGAGAAATCAAGAGGCCTTGTTCTTGTTACAGGCCCTACCG
GGAGTGGTAAATCAACTACTTTGGCGTCTATGATTGATAAGATTAATCACGAAAAAAAGGTGCACATTATTACAGTTGAA
GATCCAATTGAATACCTGCATAATCATGATAAAGCACTGATTAATCAAAGAGAGGTCAATTCAGATACAAAATCATTTGC
AAAAGCACTCAAATACATATTGAGGCAGGATCCTGATGTCGCACTTATCGGTGAAATGAGGGACCTGGAAACCATTGAAG
CGGCTTTGACTGTTTCAGAAACCGGACACCTTACTTTCGGTACACTTCATACGAACAGTGCTGTCCAAACGATAAACAGG
ATAATAGATGTTTTTCCTCCTCATCAGCAGCCTCAAATCAGAGCTCAGCTTTCTTTTGTTTTAGAGGGTGTCATCTGTCA
GCAGCTTCTTCAGACTTCCGATGGTAACAGCAGAGTGCTCGCTTGCGAGGTACTTGTACCAAACTCAGGTATTAGAAATC
TTATAAGAGAGGATAAGCTTCACCAGATATATTCTATGATGCAGTCAGGCCAGGCAGAACACGGTATGATTACAATGACA
CAGTCCTTATATGCTTTGTACAGGAAGAATTTAATTTCATATGAAGAGGCTGTTAACAGAGCTGTATATCCTGATGAAGT
TAGGCAGCTTATTGAAAGGGGAAATGTTGTCAACAAAAAGAAAAGCGGACGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F8E713

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.45

95.068

0.499
  pilT Legionella pneumophila strain Lp02

50.581

94.247

0.477
  pilT Legionella pneumophila strain ERS1305867

50.581

94.247

0.477
  pilT Acinetobacter baylyi ADP1

50

93.699

0.468
  pilT Acinetobacter baumannii strain A118

49.708

93.699

0.466
  pilT Acinetobacter baumannii D1279779

49.708

93.699

0.466
  pilT Acinetobacter nosocomialis M2

49.708

93.699

0.466
  pilT Neisseria meningitidis 8013

51.682

89.589

0.463
  pilT Neisseria gonorrhoeae MS11

51.227

89.315

0.458
  pilT Pseudomonas aeruginosa PAK

48.246

93.699

0.452
  pilT Pseudomonas stutzeri DSM 10701

48.246

93.699

0.452
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.147

91.233

0.43
  pilT Vibrio cholerae strain A1552

47.147

91.233

0.43
  pilU Vibrio cholerae strain A1552

42.09

96.986

0.408
  pilU Acinetobacter baylyi ADP1

39.945

99.452

0.397
  pilU Pseudomonas stutzeri DSM 10701

39.205

96.438

0.378

Multiple sequence alignment