Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GRX59_RS20435 Genome accession   NZ_CP047391
Coordinates   4316295..4317317 (-) Length   340 a.a.
NCBI ID   WP_060438194.1    Uniprot ID   -
Organism   Serratia marcescens strain 1602     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4311295..4322317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRX59_RS20405 (GRX59_20415) - 4312096..4312608 (+) 513 WP_033649592.1 SprT family zinc-dependent metalloprotease -
  GRX59_RS20410 (GRX59_20420) endA 4312710..4313405 (+) 696 WP_033649591.1 deoxyribonuclease I -
  GRX59_RS20415 (GRX59_20425) rsmE 4313475..4314206 (+) 732 WP_033649590.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GRX59_RS20420 (GRX59_20430) gshB 4314217..4315167 (+) 951 WP_033649589.1 glutathione synthase -
  GRX59_RS20425 (GRX59_20435) - 4315313..4315876 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  GRX59_RS20430 (GRX59_20440) ruvX 4315876..4316298 (+) 423 WP_060438193.1 Holliday junction resolvase RuvX -
  GRX59_RS20435 (GRX59_20445) pilT 4316295..4317317 (-) 1023 WP_060438194.1 type IV pilus twitching motility protein PilT Machinery gene
  GRX59_RS20440 (GRX59_20450) - 4317338..4318045 (+) 708 WP_060422989.1 YggS family pyridoxal phosphate-dependent enzyme -
  GRX59_RS20445 (GRX59_20455) proC 4318065..4318886 (+) 822 WP_033635943.1 pyrroline-5-carboxylate reductase -
  GRX59_RS20450 (GRX59_20460) - 4318918..4319472 (+) 555 WP_033635944.1 YggT family protein -
  GRX59_RS20455 (GRX59_20465) yggU 4319469..4319759 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  GRX59_RS20460 (GRX59_20470) - 4319777..4320370 (+) 594 WP_033635946.1 XTP/dITP diphosphatase -
  GRX59_RS20465 (GRX59_20475) hemW 4320363..4321505 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  GRX59_RS24565 (GRX59_20480) - 4321542..4321628 (-) 87 Protein_4016 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36450.68 Da        Isoelectric Point: 6.8349

>NTDB_id=413188 GRX59_RS20435 WP_060438194.1 4316295..4317317(-) (pilT) [Serratia marcescens strain 1602]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRQGQVDLALHRPGGE
RLRVNVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSQSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=413188 GRX59_RS20435 WP_060438194.1 4316295..4317317(-) (pilT) [Serratia marcescens strain 1602]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCAGGGGCAGGTCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGTCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCCTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGTTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGACGTTCGCTGATCCAGCAGCGGGAAATCGGCCGCGACAGTCAAAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAACTGCGCGATATTGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

51.662

97.353

0.503
  pilT Neisseria gonorrhoeae MS11

50.298

98.824

0.497
  pilT Neisseria meningitidis 8013

50.904

97.647

0.497
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.258

99.118

0.488
  pilT Legionella pneumophila strain Lp02

49.699

97.647

0.485
  pilT Legionella pneumophila strain ERS1305867

49.699

97.647

0.485
  pilT Pseudomonas aeruginosa PAK

50

97.059

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.101

100

0.462
  pilU Vibrio cholerae strain A1552

40.184

95.882

0.385
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.198

95.294

0.374

Multiple sequence alignment