Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EAE_RS03210 Genome accession   NC_015663
Coordinates   656515..657495 (-) Length   326 a.a.
NCBI ID   WP_015703454.1    Uniprot ID   A0A0H3FRT9
Organism   Klebsiella aerogenes KCTC 2190     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 651515..662495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAE_RS03180 (EAE_03105) endA 651626..652333 (+) 708 WP_015369737.1 deoxyribonuclease I -
  EAE_RS03185 (EAE_03110) rsmE 652422..653153 (+) 732 WP_015369736.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EAE_RS03190 (EAE_03115) gshB 653202..654152 (+) 951 WP_015703453.1 glutathione synthase -
  EAE_RS03195 (EAE_03120) - 654284..654847 (+) 564 WP_015369734.1 YqgE/AlgH family protein -
  EAE_RS03200 (EAE_03125) ruvX 654847..655263 (+) 417 WP_015369733.1 Holliday junction resolvase RuvX -
  EAE_RS03205 (EAE_03130) - 655511..656164 (-) 654 WP_015369732.1 LuxR C-terminal-related transcriptional regulator -
  EAE_RS03210 (EAE_03135) pilT 656515..657495 (-) 981 WP_015703454.1 type IV pilus twitching motility protein PilT Machinery gene
  EAE_RS03215 (EAE_03140) - 657512..658216 (+) 705 WP_015703455.1 YggS family pyridoxal phosphate-dependent enzyme -
  EAE_RS03220 (EAE_03145) - 658234..658800 (+) 567 WP_015369729.1 YggT family protein -
  EAE_RS03225 (EAE_03150) yggU 658797..659087 (+) 291 WP_015369728.1 DUF167 family protein YggU -
  EAE_RS03230 (EAE_03155) - 659100..659693 (+) 594 WP_015369727.1 XTP/dITP diphosphatase -
  EAE_RS03235 (EAE_03160) hemW 659686..660825 (+) 1140 WP_015703456.1 radical SAM family heme chaperone HemW -
  EAE_RS03240 (EAE_03165) - 661118..662164 (+) 1047 WP_015369725.1 sugar ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35403.52 Da        Isoelectric Point: 6.4967

>NTDB_id=41298 EAE_RS03210 WP_015703454.1 656515..657495(-) (pilT) [Klebsiella aerogenes KCTC 2190]
MELEEIVALSVKHNVSDLHLCSAYPPRWRRLGKLEPAPFTSPDVEEILTRWLNDPQLAQWRADGQVDFALALSDGPRLRA
SAFAHARGLSLALRLLPQRCPQLGDLGAPAALSELLNEESGLILVTGATGSGKSTTLAAMVDYLNQYRHGHILTLEDPVE
FIHASRNCLIQQREIGRHCSSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRSAAQAVERLVDV
FPAEEKEPVRSQLAGSLCAVAAQKLLPNASGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTSQQAGMQTFAQSFQQR
ALAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=41298 EAE_RS03210 WP_015703454.1 656515..657495(-) (pilT) [Klebsiella aerogenes KCTC 2190]
ATGGAGCTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGCGCGTATCCGCCGCG
CTGGCGTCGTTTGGGGAAGCTTGAGCCTGCACCGTTTACTTCGCCGGATGTTGAGGAAATCCTCACTCGCTGGCTTAACG
ACCCACAGTTGGCGCAGTGGCGTGCCGATGGCCAGGTGGACTTTGCCCTTGCCCTCTCAGATGGGCCGCGGCTGCGTGCC
AGCGCTTTTGCCCACGCGCGCGGCTTGTCGTTGGCGCTGCGTCTTTTGCCGCAGCGATGCCCGCAGTTGGGTGATTTGGG
CGCGCCCGCCGCGCTTAGCGAACTGTTAAATGAAGAGAGTGGTTTGATCCTGGTTACCGGCGCTACCGGTAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGACTACCTCAATCAATATCGCCATGGTCATATTTTAACCCTTGAGGATCCCGTGGAG
TTTATTCACGCCAGCAGGAACTGCCTGATCCAGCAGCGCGAAATCGGCCGCCATTGCTCCTCCTTCGCGGCGGCGCTACG
CGTGGCGCTGCGTCAGGACCCGGACGTGATCTTATTAGGTGAGCTACGCGATAGCGAAACCATCCGCCTGGCGCTGACCG
CGGCCGAAACCGGGCACCTGGTGATGGCGACGCTGCATACCCGCAGCGCGGCGCAGGCCGTCGAGCGGCTGGTGGATGTG
TTCCCGGCGGAAGAAAAAGAGCCGGTTCGCAGTCAGCTGGCAGGGAGTTTATGCGCCGTGGCGGCGCAAAAATTGTTGCC
AAATGCTTCCGGCGGCCGGGTTGCGCTATACGAACTTTTAGTGAACACGCCAGCGGTGGCAAATTTGATCCGCGAAGGAA
AAACGCATCAGCTACCCGGCGTGATGCAAACCAGTCAACAGGCAGGAATGCAAACCTTTGCGCAGAGTTTTCAACAACGT
GCGTTAGCAGGTGAGTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3FRT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.484

98.773

0.518
  pilT Vibrio cholerae strain A1552

52.484

98.773

0.518
  pilT Neisseria meningitidis 8013

49.379

98.773

0.488
  pilT Legionella pneumophila strain Lp02

49.686

97.546

0.485
  pilT Legionella pneumophila strain ERS1305867

49.686

97.546

0.485
  pilT Neisseria gonorrhoeae MS11

49.068

98.773

0.485
  pilT Acinetobacter baumannii D1279779

47.826

98.773

0.472
  pilT Acinetobacter nosocomialis M2

47.826

98.773

0.472
  pilT Acinetobacter baumannii strain A118

47.826

98.773

0.472
  pilT Pseudomonas stutzeri DSM 10701

48.734

96.933

0.472
  pilT Acinetobacter baylyi ADP1

47.205

98.773

0.466
  pilT Pseudomonas aeruginosa PAK

47.468

96.933

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.426

97.239

0.442
  pilU Pseudomonas stutzeri DSM 10701

41.515

100

0.42
  pilU Vibrio cholerae strain A1552

41.925

98.773

0.414
  pilU Acinetobacter baylyi ADP1

38.006

98.466

0.374

Multiple sequence alignment