Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H6S61_RS06575 Genome accession   NZ_CP060047
Coordinates   1299871..1300908 (-) Length   345 a.a.
NCBI ID   WP_026050359.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2497-87     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1294871..1305908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS06550 (H6S61_06550) rsmE 1295895..1296626 (+) 732 WP_185891925.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H6S61_RS06555 (H6S61_06555) gshB 1296641..1297594 (+) 954 WP_185891927.1 glutathione synthase -
  H6S61_RS06560 (H6S61_06560) - 1297648..1298211 (+) 564 WP_185891929.1 YqgE/AlgH family protein -
  H6S61_RS06565 (H6S61_06565) ruvX 1298233..1298655 (+) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  H6S61_RS06570 (H6S61_06570) pilU 1298746..1299852 (-) 1107 WP_039449206.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H6S61_RS06575 (H6S61_06575) pilT 1299871..1300908 (-) 1038 WP_026050359.1 type IV pilus twitching motility protein PilT Machinery gene
  H6S61_RS06580 (H6S61_06580) - 1300933..1301643 (+) 711 WP_185891931.1 YggS family pyridoxal phosphate-dependent enzyme -
  H6S61_RS06585 (H6S61_06585) proC 1301678..1302496 (+) 819 WP_185891933.1 pyrroline-5-carboxylate reductase -
  H6S61_RS06590 (H6S61_06590) - 1302538..1303095 (+) 558 WP_039470116.1 YggT family protein -
  H6S61_RS06595 (H6S61_06595) yggU 1303095..1303385 (+) 291 WP_013571094.1 DUF167 family protein YggU -
  H6S61_RS06600 (H6S61_06600) - 1303428..1303859 (+) 432 WP_038941482.1 DUF4426 domain-containing protein -
  H6S61_RS06605 (H6S61_06605) - 1303870..1304472 (+) 603 WP_038964745.1 XTP/dITP diphosphatase -
  H6S61_RS06610 (H6S61_06610) hemW 1304482..1305654 (+) 1173 WP_072600703.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38533.19 Da        Isoelectric Point: 6.3696

>NTDB_id=412712 H6S61_RS06575 WP_026050359.1 1299871..1300908(-) (pilT) [Vibrio vulnificus strain 2497-87]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGVPAFNHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTNIPTLEELECPQIFEKIAKCEKGLVLVTGPTGSGKSTTLAAMVDYINRNYNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGKVAKEEVDAKIEIETIQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=412712 H6S61_RS06575 WP_026050359.1 1299871..1300908(-) (pilT) [Vibrio vulnificus strain 2497-87]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAACGCATCAGATCTACATCTTTCTGCGGGTGTGCCTCCTAT
GGTACGCATAGATGGCGATGTAAGAAAGCTCGGTGTGCCCGCATTTAATCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAATTGGAAGTCGATTTTTCCTTTGAATTGCCTAACGTAGGTCGC
TTTCGTGTTAACGCCTTCAACCAATCTCGTGGCTGCTCTGCGGTGTTTCGTACCATTCCAACCAATATCCCTACCTTGGA
AGAGCTTGAGTGCCCGCAAATTTTTGAAAAAATTGCGAAGTGTGAAAAAGGCTTGGTGCTGGTCACCGGGCCGACAGGTT
CAGGTAAATCAACCACGTTAGCGGCGATGGTGGATTACATTAACCGAAACTACAACAAACACATTCTGACCATTGAAGAT
CCGATTGAATTTGTTCACCAAAATCAGAAATGTTTGATTAACCAACGCGAAGTTCATCGCGACACTCACAGTTTTAAAAA
TGCGTTGCGCAGTGCATTGCGTGAAGACCCTGATGTGATTTTAGTGGGTGAGTTACGTGACCAAGAGACCATCAGCCTAG
CGCTCACCGCGGCAGAAACGGGCCACTTGGTGTTTGGTACGTTGCACACCAGTAGTGCAGCAAAAACCATTGACCGTATT
ATTGATGTGTTCCCTGGCAGTGACAAAGACATGGTGCGCTCAATGCTTTCTGAATCACTGCGCGCCGTTATCGCCCAGAA
ACTGCTGAAACGCATTGGAGGCGGGCGAGTGGCGTGTCATGAAATCATGATGGCGACGCCTGCCATTCGTAACTTGATCC
GTGAAGATAAAGTGGCACAGATGTACTCCATTATCCAAACAGGGGCTGCGCACGGCATGCAGACGATGGAACAGAATGCG
CGTCAGTTGATGGCACAGGGCAAAGTGGCCAAAGAAGAAGTCGACGCCAAAATTGAGATTGAAACTATCCAGTTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.116

100

0.881
  pilT Vibrio cholerae strain A1552

88.116

100

0.881
  pilT Acinetobacter baumannii D1279779

72.892

96.232

0.701
  pilT Acinetobacter baumannii strain A118

72.892

96.232

0.701
  pilT Acinetobacter nosocomialis M2

72.59

96.232

0.699
  pilT Acinetobacter baylyi ADP1

72.477

94.783

0.687
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

68.58

95.942

0.658
  pilT Legionella pneumophila strain ERS1305867

68.58

95.942

0.658
  pilT Neisseria meningitidis 8013

66.066

96.522

0.638
  pilT Neisseria gonorrhoeae MS11

65.766

96.522

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.088

92.174

0.499
  pilU Pseudomonas stutzeri DSM 10701

42.515

96.812

0.412
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371