Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GEOTH_RS05085 Genome accession   NC_015660
Coordinates   996491..997549 (+) Length   352 a.a.
NCBI ID   WP_013400307.1    Uniprot ID   A0AAN0YSH4
Organism   Parageobacillus thermoglucosidasius C56-YS93     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 991491..1002549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GEOTH_RS05070 (Geoth_1009) - 992643..993425 (+) 783 WP_003248865.1 PRC-barrel domain-containing protein -
  GEOTH_RS05075 (Geoth_1010) - 993447..994805 (+) 1359 WP_013876516.1 VanW family protein -
  GEOTH_RS05080 (Geoth_1011) - 994809..996479 (+) 1671 WP_013876517.1 GspE/PulE family protein -
  GEOTH_RS05085 (Geoth_1012) pilT 996491..997549 (+) 1059 WP_013400307.1 type IV pilus twitching motility protein PilT Machinery gene
  GEOTH_RS05090 (Geoth_1013) - 997536..998747 (+) 1212 WP_041270041.1 type II secretion system F family protein -
  GEOTH_RS05095 (Geoth_1014) - 998841..999308 (+) 468 WP_013876519.1 pilus assembly FimT family protein -
  GEOTH_RS05100 (Geoth_1015) - 999319..1000065 (+) 747 WP_041270188.1 prepilin peptidase -
  GEOTH_RS05105 (Geoth_1016) pilM 1000086..1001033 (+) 948 WP_003248874.1 type IV pilus biogenesis protein PilM -
  GEOTH_RS05110 (Geoth_1017) - 1001017..1001619 (+) 603 WP_233504801.1 fimbrial protein -
  GEOTH_RS05115 (Geoth_1018) - 1001616..1002326 (+) 711 WP_013876522.1 type 4a pilus biogenesis protein PilO -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38803.97 Da        Isoelectric Point: 7.9022

>NTDB_id=41258 GEOTH_RS05085 WP_013400307.1 996491..997549(+) (pilT) [Parageobacillus thermoglucosidasius C56-YS93]
MKQRLDALLCAAFEVKASDVHLTVGVPPIFRINGELKKYGQDVLRPEDTEGMAKAVIPPRLWPQFEENGELDFSYGIPGV
SRFRINTYKQRSCVSLAIRIIPTKIPTIDELRLPEVLKQIAQKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYLHKHGSCIIDQREVGFDTNNFANGLRAALRQDPDVILVGEMRDLETIHTAITAAETGHLVLGTLHTSSAPATID
RIIDVFPPAQQSQIRIQLASVLVAIISQRLFPTPQKTGRIAATEVLINNAAVANLIRNEKVHQIANVMQTSRALGMHTLA
MSIKELMQAGLILRDAAEPYLAQERGDSYASV

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=41258 GEOTH_RS05085 WP_013400307.1 996491..997549(+) (pilT) [Parageobacillus thermoglucosidasius C56-YS93]
ATGAAGCAAAGATTGGATGCTCTTTTATGTGCTGCGTTTGAAGTTAAAGCTTCCGACGTTCATTTGACGGTCGGCGTGCC
GCCGATTTTTCGCATCAATGGCGAATTAAAAAAATACGGCCAAGACGTTTTGCGGCCGGAAGATACGGAAGGAATGGCAA
AAGCGGTCATTCCGCCCCGCCTTTGGCCGCAATTTGAAGAAAATGGGGAATTGGATTTTTCGTACGGAATTCCCGGCGTT
TCCCGCTTCCGCATCAATACGTATAAACAGCGGTCGTGCGTGTCGCTGGCGATCCGCATCATTCCGACAAAAATTCCAAC
GATTGATGAACTGCGCCTTCCTGAAGTATTAAAACAAATTGCGCAAAAACCGCACGGGCTTGTGCTAGTGACAGGCCCGA
CGGGAAGCGGGAAGTCGACGACGCTTGCTGCGATGATTGACTATATGAACAAAACGATGCGGAAACATATTATTACGCTG
GAGGATCCTATCGAATATTTGCACAAGCACGGCAGCTGCATTATTGACCAGCGCGAAGTCGGCTTTGATACGAACAATTT
TGCCAACGGTCTGCGCGCGGCACTTAGGCAAGATCCGGATGTCATTTTAGTCGGGGAAATGCGCGATTTGGAAACGATTC
ATACGGCGATAACCGCCGCGGAAACGGGACATCTCGTCCTTGGCACACTGCATACATCGAGCGCGCCGGCGACGATCGAC
CGGATTATTGACGTCTTTCCGCCTGCCCAGCAATCGCAAATCCGCATTCAGCTTGCGTCTGTTCTTGTCGCGATTATTTC
GCAGCGCCTTTTCCCAACACCGCAAAAAACGGGACGGATCGCTGCTACGGAAGTTTTAATTAATAATGCGGCAGTCGCCA
ATTTAATCCGCAATGAAAAAGTCCATCAAATCGCCAACGTCATGCAAACAAGCCGCGCCCTTGGCATGCATACGCTGGCG
ATGAGCATCAAAGAGTTAATGCAAGCAGGGCTGATCCTTCGCGATGCGGCTGAACCTTATCTTGCACAGGAGCGTGGCGA
TTCGTATGCCTCAGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.134

93.182

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

93.182

0.469
  pilT Vibrio cholerae strain A1552

50.305

93.182

0.469
  pilT Pseudomonas aeruginosa PAK

48.657

95.17

0.463
  pilT Pseudomonas stutzeri DSM 10701

48.802

94.886

0.463
  pilT Neisseria meningitidis 8013

46.377

98.011

0.455
  pilT Acinetobacter baumannii D1279779

48.485

93.75

0.455
  pilT Acinetobacter nosocomialis M2

48.485

93.75

0.455
  pilT Acinetobacter baumannii strain A118

48.485

93.75

0.455
  pilT Neisseria gonorrhoeae MS11

47.576

93.75

0.446
  pilT Acinetobacter baylyi ADP1

47.692

92.33

0.44
  pilT Legionella pneumophila strain ERS1305867

46.364

93.75

0.435
  pilT Legionella pneumophila strain Lp02

46.364

93.75

0.435
  pilU Pseudomonas stutzeri DSM 10701

44.144

94.602

0.418
  pilU Vibrio cholerae strain A1552

39.612

100

0.406
  pilU Acinetobacter baylyi ADP1

40.867

91.761

0.375

Multiple sequence alignment