Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DESKU_RS09765 Genome accession   NC_015573
Coordinates   1973048..1974148 (-) Length   366 a.a.
NCBI ID   WP_013823056.1    Uniprot ID   A0AAU8PBN5
Organism   Desulfofundulus kuznetsovii DSM 6115     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1968048..1979148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESKU_RS09735 (Desku_1978) - 1968174..1968650 (-) 477 WP_013823051.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DESKU_RS09740 (Desku_1979) - 1968656..1969438 (-) 783 WP_041282884.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DESKU_RS17860 (Desku_1980) - 1969517..1969951 (-) 435 WP_013823052.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DESKU_RS09750 (Desku_1981) - 1970170..1971159 (+) 990 WP_353928844.1 O-antigen ligase family protein -
  DESKU_RS17865 (Desku_1982) - 1971164..1971646 (-) 483 WP_013823054.1 type II secretion system protein GspG -
  DESKU_RS09760 (Desku_1983) - 1971803..1973041 (-) 1239 WP_013823055.1 type II secretion system F family protein -
  DESKU_RS09765 (Desku_1984) pilT 1973048..1974148 (-) 1101 WP_013823056.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DESKU_RS09770 (Desku_1985) gspE 1974145..1975812 (-) 1668 WP_013823057.1 type II secretion system ATPase GspE -
  DESKU_RS18925 (Desku_1986) - 1975827..1975991 (-) 165 WP_353928460.1 hypothetical protein -
  DESKU_RS09775 (Desku_1987) - 1975979..1976740 (-) 762 WP_013823059.1 A24 family peptidase -
  DESKU_RS09780 (Desku_1988) - 1976891..1977469 (-) 579 WP_013823060.1 Uma2 family endonuclease -
  DESKU_RS09785 (Desku_1989) aroB 1977502..1978590 (-) 1089 WP_013823061.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40630.10 Da        Isoelectric Point: 8.0896

>NTDB_id=40951 DESKU_RS09765 WP_013823056.1 1973048..1974148(-) (pilT) [Desulfofundulus kuznetsovii DSM 6115]
MKAVDIIAQAAKLNTSDVHLTVGLPPVYRINGVLLPLDAPELKGKLDVIKEEQIKALAPEDTQALAREIMTPDQYARFKE
KGELDFSYGVPGVTRVRVNVFRQRGSVAMVMRLLSTRIPTFQELGLPEVLGYLARRPNGLVLVTGPTGSGKSTTLAAMID
LINREKRLHIITLEDPIEYLHKHNLCIINQREIGQDTQSFASALRAALREDPDVILVGEMRDLETIATAITAAETGHLVL
ATLHTSSAAETIDRIIDVFPPSQQQQIRIQLANTIEGIVSQQLIPRHDRPGRVLALEVMVATPAIRNLIREGKTYQIPSQ
MQTGARYGMQTMDTSLRLLYQKGMISREELLNRARDPETLQRMIGG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=40951 DESKU_RS09765 WP_013823056.1 1973048..1974148(-) (pilT) [Desulfofundulus kuznetsovii DSM 6115]
ATGAAGGCCGTGGATATTATTGCCCAGGCGGCAAAATTAAATACTTCGGATGTTCACCTTACCGTGGGGCTGCCGCCTGT
CTACCGCATTAACGGTGTTTTGCTGCCGCTGGATGCACCCGAACTGAAAGGCAAACTGGACGTGATCAAGGAAGAGCAAA
TAAAGGCACTGGCGCCGGAAGACACCCAGGCGCTGGCCAGGGAAATCATGACCCCGGACCAGTATGCCCGGTTTAAAGAA
AAGGGCGAGCTTGACTTTTCTTACGGAGTGCCCGGCGTGACAAGAGTACGGGTGAACGTTTTTCGCCAGCGGGGCAGTGT
GGCCATGGTCATGCGGCTCCTGAGTACCAGGATACCCACCTTCCAGGAACTGGGTTTGCCGGAGGTGCTGGGATACCTGG
CCAGGAGGCCCAACGGCCTGGTCCTGGTCACCGGCCCCACGGGCAGCGGTAAATCCACCACCCTGGCAGCCATGATTGAT
TTAATCAACCGGGAGAAAAGGCTGCACATTATTACCCTGGAAGATCCCATTGAGTACCTGCACAAGCACAACCTCTGCAT
CATTAATCAGCGGGAAATAGGCCAGGATACTCAATCATTTGCCAGCGCCCTCCGGGCTGCCCTGCGCGAGGACCCCGACG
TCATCCTGGTGGGGGAAATGCGGGATCTGGAAACCATTGCCACGGCCATCACCGCCGCCGAAACGGGCCACTTGGTGCTG
GCCACGCTGCACACTTCCAGCGCGGCGGAAACCATTGACCGCATCATCGACGTGTTCCCGCCCAGCCAGCAGCAGCAAAT
CCGCATCCAGCTGGCCAACACCATCGAGGGAATAGTGTCCCAGCAGCTTATCCCCAGGCACGACAGGCCCGGCCGGGTAC
TGGCCCTGGAGGTAATGGTGGCCACCCCGGCCATAAGGAACCTGATCCGGGAGGGGAAGACCTACCAGATCCCATCCCAG
ATGCAGACGGGAGCCCGCTATGGCATGCAAACAATGGACACCTCCCTGCGCCTGCTCTACCAGAAAGGGATGATCAGCAG
GGAAGAGCTCCTGAACCGGGCCCGCGATCCGGAAACGCTGCAGCGTATGATAGGGGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-285)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.167

98.361

0.533
  pilT Pseudomonas aeruginosa PAK

49.444

98.361

0.486
  pilT Pseudomonas stutzeri DSM 10701

48.333

98.361

0.475
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.008

96.448

0.473
  pilT Vibrio cholerae strain A1552

49.008

96.448

0.473
  pilT Acinetobacter baylyi ADP1

47.5

98.361

0.467
  pilT Acinetobacter baumannii D1279779

47.222

98.361

0.464
  pilT Acinetobacter baumannii strain A118

47.222

98.361

0.464
  pilT Acinetobacter nosocomialis M2

47.222

98.361

0.464
  pilT Legionella pneumophila strain Lp02

47.207

97.814

0.462
  pilT Legionella pneumophila strain ERS1305867

47.207

97.814

0.462
  pilT Neisseria meningitidis 8013

46.111

98.361

0.454
  pilT Neisseria gonorrhoeae MS11

45.833

98.361

0.451
  pilU Acinetobacter baylyi ADP1

40.548

99.727

0.404
  pilU Pseudomonas stutzeri DSM 10701

42.12

95.355

0.402
  pilU Vibrio cholerae strain A1552

41.261

95.355

0.393

Multiple sequence alignment