Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPY28_RS24005 Genome accession   NZ_CP046814
Coordinates   3154299..3155339 (-) Length   346 a.a.
NCBI ID   WP_005381015.1    Uniprot ID   A0A2I3C9Z7
Organism   Vibrio alginolyticus strain 2010V-1102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3149299..3160339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY28_RS23975 endA 3149442..3150137 (+) 696 WP_017635582.1 deoxyribonuclease I -
  GPY28_RS23980 rsmE 3150251..3150982 (+) 732 WP_053350809.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GPY28_RS23985 gshB 3150997..3151947 (+) 951 WP_005381021.1 glutathione synthase -
  GPY28_RS23990 - 3152076..3152639 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  GPY28_RS23995 ruvX 3152675..3153100 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  GPY28_RS24000 pilU 3153162..3154268 (-) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPY28_RS24005 pilT 3154299..3155339 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  GPY28_RS24010 - 3155368..3156069 (+) 702 WP_062730830.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPY28_RS24015 proC 3156229..3157047 (+) 819 WP_062730829.1 pyrroline-5-carboxylate reductase -
  GPY28_RS24020 - 3157100..3157657 (+) 558 WP_005381012.1 YggT family protein -
  GPY28_RS24025 yggU 3157657..3157947 (+) 291 WP_005381011.1 DUF167 family protein YggU -
  GPY28_RS24030 - 3158076..3158507 (+) 432 WP_053350811.1 DUF4426 domain-containing protein -
  GPY28_RS24035 - 3158626..3159228 (+) 603 WP_006743095.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38532.02 Da        Isoelectric Point: 6.5039

>NTDB_id=408245 GPY28_RS24005 WP_005381015.1 3154299..3155339(-) (pilT) [Vibrio alginolyticus strain 2010V-1102]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=408245 GPY28_RS24005 WP_005381015.1 3154299..3155339(-) (pilT) [Vibrio alginolyticus strain 2010V-1102]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTAAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATTAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
CGCACTGCGCAGCGCATTGCGCGAAGACCCAGACGTTATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCACACCAGCTCGGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTTCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGCAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361

Multiple sequence alignment