Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THEXY_RS05945 Genome accession   NC_015555
Coordinates   1233117..1234169 (+) Length   350 a.a.
NCBI ID   WP_013787931.1    Uniprot ID   -
Organism   Thermoanaerobacterium xylanolyticum LX-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1228117..1239169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEXY_RS05925 (Thexy_1152) sigK 1229117..1229812 (+) 696 WP_013787927.1 RNA polymerase sporulation sigma factor SigK -
  THEXY_RS05930 (Thexy_1153) - 1229896..1230390 (+) 495 WP_013787928.1 YqeG family HAD IIIA-type phosphatase -
  THEXY_RS05935 (Thexy_1154) aroE 1230395..1231246 (+) 852 WP_013787929.1 shikimate dehydrogenase -
  THEXY_RS05940 (Thexy_1155) - 1231395..1233089 (+) 1695 WP_013787930.1 ATPase, T2SS/T4P/T4SS family -
  THEXY_RS05945 (Thexy_1156) pilT 1233117..1234169 (+) 1053 WP_013787931.1 type IV pilus twitching motility protein PilT Machinery gene
  THEXY_RS05950 (Thexy_1157) - 1234195..1235409 (+) 1215 WP_013787932.1 type II secretion system F family protein -
  THEXY_RS05955 (Thexy_1158) - 1235437..1235799 (+) 363 WP_013787933.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEXY_RS05960 (Thexy_1159) - 1235854..1236609 (+) 756 WP_013787934.1 A24 family peptidase -
  THEXY_RS05965 (Thexy_1160) - 1236614..1237078 (+) 465 WP_013787935.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEXY_RS12370 (Thexy_1161) - 1237090..1237827 (+) 738 WP_013787936.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEXY_RS05975 (Thexy_1162) - 1237805..1238269 (+) 465 WP_013787937.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEXY_RS05980 (Thexy_1163) - 1238280..1238882 (+) 603 WP_013787938.1 hypothetical protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38756.99 Da        Isoelectric Point: 6.3111

>NTDB_id=40742 THEXY_RS05945 WP_013787931.1 1233117..1234169(+) (pilT) [Thermoanaerobacterium xylanolyticum LX-11]
MKTSELLAMVVEKGASDLHITVGVPPVLRINGQLIKLDLPQLTPQDTEEITKDLLSSDELKKLEDAGDIDLSYSVKGLGR
FRINAYKQRGTYSLAIRSVALRIPTIDELGLPEVIKDLALKTRGLIIVTGPTGSGKSTTLASMIDLINEERNCHILTLED
PIEYLHKHKKSIVNQREIGHDALSYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGSAKTIDRI
IDVFPPHQQQQIKVQLSNVLEGIISQQLLPKIDNSGRVVAVEVMIATPAIRNLIREGKSFQIQSMVQTGNKFGMVTMDMW
ISQLLKRNLISMDDALTYCVDRENFSRLVV

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=40742 THEXY_RS05945 WP_013787931.1 1233117..1234169(+) (pilT) [Thermoanaerobacterium xylanolyticum LX-11]
ATGAAAACAAGTGAACTTCTGGCTATGGTTGTAGAAAAAGGCGCATCTGATTTGCATATAACAGTTGGCGTTCCTCCGGT
ACTTAGAATTAATGGGCAACTTATAAAACTGGATTTACCTCAATTAACACCTCAAGATACTGAAGAAATAACAAAAGATT
TGCTTTCAAGCGATGAGCTAAAAAAACTTGAAGATGCAGGTGATATTGACCTATCGTATTCAGTAAAAGGGCTAGGAAGG
TTTAGGATAAATGCTTATAAGCAAAGGGGAACATATAGCCTTGCCATAAGATCTGTCGCTTTGAGAATTCCGACAATTGA
TGAATTAGGACTTCCAGAAGTGATTAAAGATCTTGCGCTTAAAACCCGTGGTCTCATAATTGTTACAGGGCCTACAGGCA
GCGGAAAATCTACGACATTGGCATCTATGATCGATTTAATAAACGAAGAACGTAATTGTCATATATTGACCCTGGAAGAT
CCTATTGAATATTTGCATAAGCACAAAAAAAGTATAGTAAATCAAAGAGAAATCGGGCATGATGCTTTGTCATACGCCAG
TGCATTAAGGGCAGCATTAAGAGAGGACCCTGATGTGATACTGGTTGGAGAAATGAGGGATCTTGAGACGATACAGATAG
CTATAACAGCGGCTGAGACGGGGCATCTTGTGTTATCCACTTTACATACCATAGGTTCGGCAAAGACTATCGACAGGATT
ATAGATGTTTTTCCGCCGCATCAGCAACAGCAGATAAAAGTACAGCTTTCAAATGTCTTAGAAGGGATTATATCGCAGCA
ATTATTGCCTAAAATAGATAATTCAGGGCGAGTCGTCGCCGTAGAAGTTATGATAGCTACACCTGCAATAAGAAATTTAA
TAAGAGAAGGGAAGTCATTTCAAATACAATCGATGGTGCAGACAGGGAATAAATTTGGAATGGTGACAATGGATATGTGG
ATATCGCAGCTATTAAAGAGAAATTTGATTTCTATGGACGATGCACTTACATATTGTGTTGATAGAGAAAACTTTTCTCG
ATTAGTAGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.857

0.494
  pilT Pseudomonas aeruginosa PAK

49.275

98.571

0.486
  pilT Pseudomonas stutzeri DSM 10701

48.696

98.571

0.48
  pilT Acinetobacter nosocomialis M2

48.696

98.571

0.48
  pilT Acinetobacter baumannii D1279779

48.696

98.571

0.48
  pilT Acinetobacter baumannii strain A118

48.696

98.571

0.48
  pilT Legionella pneumophila strain Lp02

48.696

98.571

0.48
  pilT Legionella pneumophila strain ERS1305867

48.696

98.571

0.48
  pilT Acinetobacter baylyi ADP1

48.406

98.571

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.485

94.286

0.457
  pilT Vibrio cholerae strain A1552

48.485

94.286

0.457
  pilT Neisseria meningitidis 8013

45.821

99.143

0.454
  pilT Neisseria gonorrhoeae MS11

45.087

98.857

0.446
  pilU Pseudomonas stutzeri DSM 10701

40.286

100

0.403
  pilU Vibrio cholerae strain A1552

40.171

100

0.403
  pilU Acinetobacter baylyi ADP1

38.746

100

0.389

Multiple sequence alignment