Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MAHAU_RS05630 Genome accession   NC_015520
Coordinates   1173709..1174746 (+) Length   345 a.a.
NCBI ID   WP_148258470.1    Uniprot ID   -
Organism   Mahella australiensis 50-1 BON     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1168709..1179746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MAHAU_RS05600 (Mahau_1125) - 1170207..1170542 (+) 336 WP_013780752.1 type II secretion system protein -
  MAHAU_RS05605 (Mahau_1126) - 1170552..1171046 (+) 495 WP_013780753.1 type II secretion system protein -
  MAHAU_RS05610 (Mahau_1127) pilM 1171071..1172114 (+) 1044 WP_013780754.1 type IV pilus assembly protein PilM -
  MAHAU_RS05615 (Mahau_1128) - 1172118..1172639 (+) 522 WP_013780755.1 PilN domain-containing protein -
  MAHAU_RS05620 (Mahau_1129) - 1172653..1173198 (+) 546 WP_013780756.1 hypothetical protein -
  MAHAU_RS05625 (Mahau_1130) - 1173188..1173709 (+) 522 WP_013780757.1 shikimate kinase -
  MAHAU_RS05630 (Mahau_1131) pilT 1173709..1174746 (+) 1038 WP_148258470.1 type IV pilus twitching motility protein PilT Machinery gene
  MAHAU_RS05635 (Mahau_1132) - 1174767..1176449 (+) 1683 WP_013780759.1 GspE/PulE family protein -
  MAHAU_RS05640 (Mahau_1133) - 1176467..1177684 (+) 1218 WP_013780760.1 type II secretion system F family protein -
  MAHAU_RS05645 (Mahau_1134) - 1177689..1178456 (+) 768 WP_013780761.1 A24 family peptidase -
  MAHAU_RS05650 (Mahau_1135) - 1178474..1178926 (+) 453 WP_013780762.1 GspH/FimT family pseudopilin -
  MAHAU_RS05655 (Mahau_1136) - 1178923..1179291 (+) 369 WP_013780763.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MAHAU_RS05660 (Mahau_1137) - 1179297..1179734 (+) 438 WP_281004402.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37828.67 Da        Isoelectric Point: 6.9765

>NTDB_id=40710 MAHAU_RS05630 WP_148258470.1 1173709..1174746(+) (pilT) [Mahella australiensis 50-1 BON]
MFINELLKETVARKASDLHITVRMPPVIRANGALTPLPGEALSPEDTENLVRQMMGQRQLEQLAEEGECDFSFSVAGLGR
FRVNVYRQRGTYGAAIRALPAQPPAIDSLGLPDVVRQLAETNVGIVLVTGPTGSGKSTTLAAMIDYINEHRSCHILTMED
PIEYLHKHKKSIVNQREIGSDTKSYAAALRAALREDPDVILVGEMRDLEAISIAITAAETGHLVLSTLHTIGAARTIDRI
VDVFPPHQQQQIRVQLSMVIQGVISQQLMPRADGTGRVVAAETMLATPAIRNLIRENKTYQIISAMQTGTRLGMRTMDSA
IQELYSKGIITKETAAMHFVEKSVM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=40710 MAHAU_RS05630 WP_148258470.1 1173709..1174746(+) (pilT) [Mahella australiensis 50-1 BON]
ATATTTATAAACGAATTATTAAAAGAAACCGTGGCGCGCAAAGCGTCCGACCTTCATATCACTGTCCGTATGCCGCCTGT
TATAAGGGCTAACGGCGCTCTTACGCCGCTGCCCGGCGAAGCGTTATCGCCTGAGGATACCGAGAACCTGGTGCGTCAGA
TGATGGGGCAGAGGCAATTGGAACAACTTGCTGAAGAGGGCGAATGCGACTTTTCATTTTCGGTGGCAGGGTTGGGACGA
TTCCGAGTAAATGTTTATAGGCAAAGGGGTACGTACGGTGCGGCCATACGCGCGCTGCCGGCTCAGCCTCCAGCCATAGA
CAGCTTGGGTTTGCCCGATGTCGTAAGGCAACTGGCCGAGACAAATGTAGGCATAGTACTTGTTACCGGTCCTACTGGCA
GCGGTAAGTCAACAACGCTAGCGGCAATGATAGACTATATAAACGAGCACAGAAGCTGCCATATACTTACGATGGAGGAC
CCTATAGAATATCTGCATAAGCATAAAAAGAGTATAGTGAATCAGCGAGAGATAGGCAGCGATACAAAAAGCTATGCTGC
GGCTTTAAGGGCGGCCTTGAGGGAGGACCCGGATGTTATATTGGTAGGCGAGATGCGTGACCTTGAGGCCATATCTATTG
CTATAACGGCGGCCGAGACCGGCCATCTCGTGCTCTCGACGTTGCACACCATAGGAGCGGCCAGGACCATAGACCGCATT
GTAGACGTTTTTCCGCCTCATCAACAACAGCAGATAAGGGTGCAACTATCCATGGTCATACAGGGCGTGATATCCCAGCA
GCTTATGCCAAGAGCCGACGGTACCGGCAGAGTGGTGGCTGCGGAGACTATGTTAGCCACGCCGGCTATACGCAATCTCA
TACGCGAAAATAAGACGTATCAGATAATAAGCGCCATGCAGACTGGAACCAGGCTCGGCATGAGGACTATGGACAGCGCT
ATACAGGAGCTTTACAGCAAGGGCATTATAACCAAGGAAACCGCAGCTATGCATTTTGTGGAAAAGAGCGTTATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.776

95.942

0.516
  pilT Legionella pneumophila strain ERS1305867

51.304

100

0.513
  pilT Legionella pneumophila strain Lp02

51.304

100

0.513
  pilT Acinetobacter baylyi ADP1

52.537

97.101

0.51
  pilT Acinetobacter nosocomialis M2

52.537

97.101

0.51
  pilT Acinetobacter baumannii D1279779

52.239

97.101

0.507
  pilT Acinetobacter baumannii strain A118

52.239

97.101

0.507
  pilT Pseudomonas aeruginosa PAK

51.045

97.101

0.496
  pilT Pseudomonas stutzeri DSM 10701

51.045

97.101

0.496
  pilT Neisseria meningitidis 8013

50.746

97.101

0.493
  pilT Neisseria gonorrhoeae MS11

50.448

97.101

0.49
  pilT Vibrio cholerae strain A1552

50

95.652

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

95.652

0.478
  pilU Pseudomonas stutzeri DSM 10701

43.195

97.971

0.423
  pilU Vibrio cholerae strain A1552

42.687

97.101

0.414
  pilU Acinetobacter baylyi ADP1

40.413

98.261

0.397

Multiple sequence alignment