Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GPY00_RS04250 Genome accession   NZ_CP046747
Coordinates   934681..935787 (-) Length   368 a.a.
NCBI ID   WP_000422572.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3541-04     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 929681..940787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY00_RS04225 gshB 930295..931251 (+) 957 WP_158128463.1 glutathione synthase -
  GPY00_RS04230 - 931287..931850 (+) 564 WP_005526767.1 YqgE/AlgH family protein -
  GPY00_RS04235 ruvX 931930..932352 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  GPY00_RS04240 tyrS 932377..933657 (-) 1281 WP_044126947.1 tyrosine--tRNA ligase -
  GPY00_RS04245 - 933766..934563 (-) 798 WP_001123221.1 helix-turn-helix transcriptional regulator -
  GPY00_RS04250 pilU 934681..935787 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPY00_RS04255 pilT 935802..936839 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  GPY00_RS04260 - 936865..937575 (+) 711 WP_158128464.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPY00_RS04265 proC 937628..938446 (+) 819 WP_044126945.1 pyrroline-5-carboxylate reductase -
  GPY00_RS04270 - 938500..939057 (+) 558 WP_001087261.1 YggT family protein -
  GPY00_RS04275 yggU 939057..939347 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  GPY00_RS04280 - 939393..939824 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  GPY00_RS04285 - 939894..940496 (+) 603 WP_044126943.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41307.27 Da        Isoelectric Point: 6.5076

>NTDB_id=407048 GPY00_RS04250 WP_000422572.1 934681..935787(-) (pilU) [Vibrio cholerae strain 3541-04]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=407048 GPY00_RS04250 WP_000422572.1 934681..935787(-) (pilU) [Vibrio cholerae strain 3541-04]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCGCAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAGGCGAATTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCGATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAGGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGGCATTTGTGTATGGCCACGCTGCACGCCAATAACGCCAACCAAGCGTTAGAGCGCATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GAGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

100

100

1
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment